目的 探讨甲状旁腺激素(PTH)在继发性甲状旁腺功能亢进手术中的应用价值。方法 选取2017年1月1日至2018年11月30日来该院住院的慢性肾功能不全继发甲状旁腺功能亢进,并进行甲状旁腺切除手术的患者共93例,对其进行术前、术后第1天和术后...目的 探讨甲状旁腺激素(PTH)在继发性甲状旁腺功能亢进手术中的应用价值。方法 选取2017年1月1日至2018年11月30日来该院住院的慢性肾功能不全继发甲状旁腺功能亢进,并进行甲状旁腺切除手术的患者共93例,对其进行术前、术后第1天和术后第4天血清钙、磷、全段甲状旁腺激素(iPTH)检测,并比较其变化情况。同时监测甲状旁腺切除术前、术中、术后iPTH的变化情况。比较继发性甲状旁腺功能亢进患者与50例体检健康者骨代谢指标的变化情况,包括碱性磷酸酶(ALP)、25-羟维生素D(25-OH-VD)、总Ⅰ型胶原氨基端延长肽(tPINP)、β-Ⅰ型胶原羧基端肽(β-CTx)、骨钙素(OST)。结果 术中20 min、术中30 min、术后第1天和术后第4天血清iPTH结果均较术前有所下降,差异有统计学意义( Z =-8.374、-8.374 、-8.374 、-8.374,均 P <0.001)。术后第1天和术后第4天血清磷均较术前有所下降,差异有统计学意义( Z =-8.202 、-8.371,均 P <0.001)。术后第1天和术后第4天血清钙均较术前有所下降,差异有统计学意义( Z =-7.813 、-6.190,均 P <0.001)。继发性甲状旁腺功能亢进患者ALP、tPINP、β-CTx、OST检测结果均较体检健康者偏高,差异有统计学意义( Z =-9.691、-10.373、-10.094、-9.952,均 P <0.001);继发性甲状旁腺功能亢进患者25-OH-VD检测结果与体检健康者相比,差异无统计学意义( Z =-0.50, P > 0.05 )。结论 联合检测钙、磷、iPTH及其他骨代谢指标,对肾功能不全继发性甲状旁腺功能亢进患者进行甲状旁腺切除手术有重要的指导意义,且继发性甲状旁腺功能亢进患者骨代谢指标的变化所导致的骨代谢紊乱可作为手术切除甲状旁腺的重要指征。展开更多
Vibrio vulnificus is an estuarine bacterial pathogen for human.The rapid,specific and sensitive detection of V.vulnificus is urgently needed for early disease diagnosis and timely treatment of V.vulnificus infection.I...Vibrio vulnificus is an estuarine bacterial pathogen for human.The rapid,specific and sensitive detection of V.vulnificus is urgently needed for early disease diagnosis and timely treatment of V.vulnificus infection.In the study,a loop-mediated isothermal amplification（LAMP） technique was developed for V.vulnificus detection with a set of primers,composed of two out primers and two inner primers targeted to vvh A gene.The optimal amplification temperature was 63°C and the reaction only took 35 min.The amplification products could not only be detected by agarose gel electrophoresis with ladder-like pattern bands,but also could be visualized using calcein with naked eye directly.Forty-five strains were tested for the specificity of LAMP assay,and all the V.vulnificus strains were identified correctly while other strains were negative results.The sensitive of the new LAMP assay was 100-fold more sensitive than the conventional PCR.Meanwhile,all the V.vulnificus strains were detected correctly in spiked,clinical and environmental samples by the new LAMP assay.Compared with other well-known techniques,the new LAMP assay targeted to vvh A gene was extremely rapid,simple,sensitive and specific for V.vulnificus identification.展开更多
文摘目的 探讨甲状旁腺激素(PTH)在继发性甲状旁腺功能亢进手术中的应用价值。方法 选取2017年1月1日至2018年11月30日来该院住院的慢性肾功能不全继发甲状旁腺功能亢进,并进行甲状旁腺切除手术的患者共93例,对其进行术前、术后第1天和术后第4天血清钙、磷、全段甲状旁腺激素(iPTH)检测,并比较其变化情况。同时监测甲状旁腺切除术前、术中、术后iPTH的变化情况。比较继发性甲状旁腺功能亢进患者与50例体检健康者骨代谢指标的变化情况,包括碱性磷酸酶(ALP)、25-羟维生素D(25-OH-VD)、总Ⅰ型胶原氨基端延长肽(tPINP)、β-Ⅰ型胶原羧基端肽(β-CTx)、骨钙素(OST)。结果 术中20 min、术中30 min、术后第1天和术后第4天血清iPTH结果均较术前有所下降,差异有统计学意义( Z =-8.374、-8.374 、-8.374 、-8.374,均 P <0.001)。术后第1天和术后第4天血清磷均较术前有所下降,差异有统计学意义( Z =-8.202 、-8.371,均 P <0.001)。术后第1天和术后第4天血清钙均较术前有所下降,差异有统计学意义( Z =-7.813 、-6.190,均 P <0.001)。继发性甲状旁腺功能亢进患者ALP、tPINP、β-CTx、OST检测结果均较体检健康者偏高,差异有统计学意义( Z =-9.691、-10.373、-10.094、-9.952,均 P <0.001);继发性甲状旁腺功能亢进患者25-OH-VD检测结果与体检健康者相比,差异无统计学意义( Z =-0.50, P > 0.05 )。结论 联合检测钙、磷、iPTH及其他骨代谢指标,对肾功能不全继发性甲状旁腺功能亢进患者进行甲状旁腺切除手术有重要的指导意义,且继发性甲状旁腺功能亢进患者骨代谢指标的变化所导致的骨代谢紊乱可作为手术切除甲状旁腺的重要指征。
基金The Major PLA Research Project of "The 12th Five-Year Plan" for Medical Science Development under contract No.BWS12J014the Primary Research & Development Plan of Shandong Province under contract No.2016GSF121036
文摘Vibrio vulnificus is an estuarine bacterial pathogen for human.The rapid,specific and sensitive detection of V.vulnificus is urgently needed for early disease diagnosis and timely treatment of V.vulnificus infection.In the study,a loop-mediated isothermal amplification（LAMP） technique was developed for V.vulnificus detection with a set of primers,composed of two out primers and two inner primers targeted to vvh A gene.The optimal amplification temperature was 63°C and the reaction only took 35 min.The amplification products could not only be detected by agarose gel electrophoresis with ladder-like pattern bands,but also could be visualized using calcein with naked eye directly.Forty-five strains were tested for the specificity of LAMP assay,and all the V.vulnificus strains were identified correctly while other strains were negative results.The sensitive of the new LAMP assay was 100-fold more sensitive than the conventional PCR.Meanwhile,all the V.vulnificus strains were detected correctly in spiked,clinical and environmental samples by the new LAMP assay.Compared with other well-known techniques,the new LAMP assay targeted to vvh A gene was extremely rapid,simple,sensitive and specific for V.vulnificus identification.
