The "museum fever" has become a new fashion of Chinese social culture, and the cross-border integration and innovation of "muscum+" has become a new engine for promoting high-quality development.
Insulin-like growth factor binding-protein-7 (IGFBP7) was obtained from our previous colonic adenocarcinoma (CRC) and normal mucosa suppression subtraction hybridization (SSH) cDNA libraries. By RT-PCR and immunohisto...Insulin-like growth factor binding-protein-7 (IGFBP7) was obtained from our previous colonic adenocarcinoma (CRC) and normal mucosa suppression subtraction hybridization (SSH) cDNA libraries. By RT-PCR and immunohistochemistry, we found that IGFBP7 was overexpressed in CRC tissue compared to normal tissue. However, our in vitro experiments performed in 10 CRC cell lines showed that IGFBP7 expressed only in SW480 and Caco2 cell lines, which implied an underlying reversible regulatory mechanism. Using methylation-specific PCR (MSP) and bisulfite sodium PCR (BSP), we found that its expression was associated with DNA hypomethylation of exon1. This was further supported by the in vitro study which showed restored IGFBP7 expression after demethylation agent 5-aza-2’-deoxycytidine treatment. Correlation analysis between IGFBP7 expression and prognosis indicated that overexpression of IGFBP7 in CRC tissue correlated with favourable survival. Investigation of the func-tional role of IGFBP7 through transfection studies showed that IGFBP7 protein could inhibit growth rate, decrease colony for-mation activity, and induce apoptosis in RKO and SW620 cells, suggesting it a potential tumor suppressor protein in colorectal carcinogenesis. In conclusion, our study clearly demonstrated that IGFBP7 plays a potential tumor suppressor role against colo-rectal carcinogenesis and its expression is associated with DNA hypomethylation of exon 1.展开更多
Abstract Objective To evaluate the possible vascular effects of an environment carcinogen benzo（a）pyrene （BaP）. Methods The cytotoxicit of BaP and rat liver S9 （0.25 mg/mL）-activated BaP were examined by MTT ass...Abstract Objective To evaluate the possible vascular effects of an environment carcinogen benzo（a）pyrene （BaP）. Methods The cytotoxicit of BaP and rat liver S9 （0.25 mg/mL）-activated BaP were examined by MTT assay. Thoracic aortic rings were dissected from Sprague-Dawley rats. Contraction of aortic rings was induced by 60 mmol/L KCl or 10-6 mol/L phenylephrine （PE） in an ex-vivo perfusion system after BaP （100 tlmol/L） incubation for 6 h. [Ca^2＋]i was measured using Fluo-4/AM. For in-vivo treatment, rats were injected with BaP for 4 weeks （10 mg/kg, weekly, i.p.）. Results BaP （1-500 μm） did not significantly affect cell viability; S9-activated BaP stimulated cell proliferation. BaP did not affect the contractile function of endothelium-intact or -denuded aortic rings. BaP did not affect ATP-induced （[Ca2＋]i） increases in human umbilical vein endothelial cells. In BaP-treated rats, heart rate and the number of circulating inflammatory cells were not affected. Body weight decreased while blood pressure increased significantly. The maximum aortic contractile responses to PE and KCI and the maximum aortic relaxation response to acetylcholine were significantly decreased by 25.0%, 34.2%, and 10.4%, respectively. Conclusion These results suggest, in accordance with its DNA-damaging properties, that metabolic activation is a prerequisite for BaP-induced cardiovascular toxicity.展开更多
The National Art Museum of China(NAMOC) recently opened an exhibition on its collection of extremely valuable artworks and historical archives in celebration of its 50th anniversary NAMOC is a state-level institution ...The National Art Museum of China(NAMOC) recently opened an exhibition on its collection of extremely valuable artworks and historical archives in celebration of its 50th anniversary NAMOC is a state-level institution of fine arts in China. Over the past five decades,NAMOC has made active endeavors and significant contribution to showcasing achievements of Chinese fine arts,accumulating the country’s resources in fine arts,serving the展开更多
Ntho-ling Monastery is located in Zanda County of Ngari in the western part of Tibetan Autonomous Region,about 60 kilometers west to the site of the ancient kingdom of Guge.In the Tibetan language,
On the occasion of the International Museum Day on May 18, the Ten Best Museum Exhibitions of the Year were released, a competition sponsored by the State Administration of Cultural Heritage of China and co-organized ...On the occasion of the International Museum Day on May 18, the Ten Best Museum Exhibitions of the Year were released, a competition sponsored by the State Administration of Cultural Heritage of China and co-organized by Chinae Museum Association, Xi’an Municipality and China Heritage News. The exhibition “Buddhist Sculpture Masterpieces of Ancient China” by the Capital Museum in Beijing won the “Best Production” award.展开更多
The ten national permanent museum exhibitions of the year were announced on the World Museum Day on May 18, an annual event co-organized by China Museum Association, Xi’an Municipality and China Heritage News. The pe...The ten national permanent museum exhibitions of the year were announced on the World Museum Day on May 18, an annual event co-organized by China Museum Association, Xi’an Municipality and China Heritage News. The permanent exhibition of the outer burial pits of the Yangling Mausoleum of Emperor Jingdi of the Han Dynasty won this annual award.展开更多
文摘The "museum fever" has become a new fashion of Chinese social culture, and the cross-border integration and innovation of "muscum+" has become a new engine for promoting high-quality development.
基金Project (Nos. 30200333 and 30570840) supported by the NationalNatural Science Foundation of China
文摘Insulin-like growth factor binding-protein-7 (IGFBP7) was obtained from our previous colonic adenocarcinoma (CRC) and normal mucosa suppression subtraction hybridization (SSH) cDNA libraries. By RT-PCR and immunohistochemistry, we found that IGFBP7 was overexpressed in CRC tissue compared to normal tissue. However, our in vitro experiments performed in 10 CRC cell lines showed that IGFBP7 expressed only in SW480 and Caco2 cell lines, which implied an underlying reversible regulatory mechanism. Using methylation-specific PCR (MSP) and bisulfite sodium PCR (BSP), we found that its expression was associated with DNA hypomethylation of exon1. This was further supported by the in vitro study which showed restored IGFBP7 expression after demethylation agent 5-aza-2’-deoxycytidine treatment. Correlation analysis between IGFBP7 expression and prognosis indicated that overexpression of IGFBP7 in CRC tissue correlated with favourable survival. Investigation of the func-tional role of IGFBP7 through transfection studies showed that IGFBP7 protein could inhibit growth rate, decrease colony for-mation activity, and induce apoptosis in RKO and SW620 cells, suggesting it a potential tumor suppressor protein in colorectal carcinogenesis. In conclusion, our study clearly demonstrated that IGFBP7 plays a potential tumor suppressor role against colo-rectal carcinogenesis and its expression is associated with DNA hypomethylation of exon 1.
基金supported by National Natural Science Foundation of China,grant numbers30872140and81172692Zhejiang Provincial Natural Science Foundation,R2100555Ministry of Science and Technology,China,2009DFB30390
文摘Abstract Objective To evaluate the possible vascular effects of an environment carcinogen benzo（a）pyrene （BaP）. Methods The cytotoxicit of BaP and rat liver S9 （0.25 mg/mL）-activated BaP were examined by MTT assay. Thoracic aortic rings were dissected from Sprague-Dawley rats. Contraction of aortic rings was induced by 60 mmol/L KCl or 10-6 mol/L phenylephrine （PE） in an ex-vivo perfusion system after BaP （100 tlmol/L） incubation for 6 h. [Ca^2＋]i was measured using Fluo-4/AM. For in-vivo treatment, rats were injected with BaP for 4 weeks （10 mg/kg, weekly, i.p.）. Results BaP （1-500 μm） did not significantly affect cell viability; S9-activated BaP stimulated cell proliferation. BaP did not affect the contractile function of endothelium-intact or -denuded aortic rings. BaP did not affect ATP-induced （[Ca2＋]i） increases in human umbilical vein endothelial cells. In BaP-treated rats, heart rate and the number of circulating inflammatory cells were not affected. Body weight decreased while blood pressure increased significantly. The maximum aortic contractile responses to PE and KCI and the maximum aortic relaxation response to acetylcholine were significantly decreased by 25.0%, 34.2%, and 10.4%, respectively. Conclusion These results suggest, in accordance with its DNA-damaging properties, that metabolic activation is a prerequisite for BaP-induced cardiovascular toxicity.
文摘The National Art Museum of China(NAMOC) recently opened an exhibition on its collection of extremely valuable artworks and historical archives in celebration of its 50th anniversary NAMOC is a state-level institution of fine arts in China. Over the past five decades,NAMOC has made active endeavors and significant contribution to showcasing achievements of Chinese fine arts,accumulating the country’s resources in fine arts,serving the
文摘Ntho-ling Monastery is located in Zanda County of Ngari in the western part of Tibetan Autonomous Region,about 60 kilometers west to the site of the ancient kingdom of Guge.In the Tibetan language,
文摘On the occasion of the International Museum Day on May 18, the Ten Best Museum Exhibitions of the Year were released, a competition sponsored by the State Administration of Cultural Heritage of China and co-organized by Chinae Museum Association, Xi’an Municipality and China Heritage News. The exhibition “Buddhist Sculpture Masterpieces of Ancient China” by the Capital Museum in Beijing won the “Best Production” award.
文摘The ten national permanent museum exhibitions of the year were announced on the World Museum Day on May 18, an annual event co-organized by China Museum Association, Xi’an Municipality and China Heritage News. The permanent exhibition of the outer burial pits of the Yangling Mausoleum of Emperor Jingdi of the Han Dynasty won this annual award.
