The "museum fever" has become a new fashion of Chinese social culture, and the cross-border integration and innovation of "muscum+" has become a new engine for promoting high-quality development.
The Exhibition of Cuhural Rel- ics of Shang and Zhou Dynas- ties Unearthed from Hubei Prov-ince was recently on view at the National Museum o｜China in Beijing, This exhi- bition displayed 122 pieces/sets of cul- tura...The Exhibition of Cuhural Rel- ics of Shang and Zhou Dynas- ties Unearthed from Hubei Prov-ince was recently on view at the National Museum o｜China in Beijing, This exhi- bition displayed 122 pieces/sets of cul- tural relics from the collection of Huhei Provincial Museum as well as other ten museums of Hubei province, showcas- ing the civilization of ancient China dat- ing from the early Shang Dynasty （1600 - 1046 BC）to the Warring States period （475 - 221 BC）.展开更多
Insulin-like growth factor binding-protein-7 （IGFBP7） was obtained from our previous colonic adenocarcinoma （CRC） and normal mucosa suppression subtraction hybridization （SSH） cDNA libraries. By RT-PCR and immun...Insulin-like growth factor binding-protein-7 （IGFBP7） was obtained from our previous colonic adenocarcinoma （CRC） and normal mucosa suppression subtraction hybridization （SSH） cDNA libraries. By RT-PCR and immunohistochemistry, we found that IGFBP7 was overexpressed in CRC tissue compared to normal tissue. However, our in vitro experiments performed in 10 CRC cell lines showed that IGFBP7 expressed only in SW480 and Caco2 cell lines, which implied an underlying reversible regulatory mechanism. Using methylation-specific PCR （MSP） and bisulfite sodium PCR （BSP）, we found that its expression was associated with DNA hypomethylation of exonl. This was further supported by the in vitro study which showed restored IGFBP7 expression after demethylation agent 5-aza-2＇-deoxycytidine treatment. Correlation analysis between IGFBP7 expression and prognosis indicated that overexpression of IGFBP7 in CRC tissue correlated with favourable survival. Investigation of the functional role of IGFBP7 through transfection studies showed that IGFBP7 protein could inhibit growth rate, decrease colony formation activity, and induce apoptosis in RKO and SW620 cells, suggesting it a potential tumor suppressor protein in colorectal carcinogenesis. In conclusion, our study clearly demonstrated that IGFBP7 plays a potential tumor suppressor role against colorectal carcinogenesis and its expression is associated with DNA hypomethylation of exon 1.展开更多
Abstract Objective To evaluate the possible vascular effects of an environment carcinogen benzo（a）pyrene （BaP）. Methods The cytotoxicit of BaP and rat liver S9 （0.25 mg/mL）-activated BaP were examined by MTT ass...Abstract Objective To evaluate the possible vascular effects of an environment carcinogen benzo（a）pyrene （BaP）. Methods The cytotoxicit of BaP and rat liver S9 （0.25 mg/mL）-activated BaP were examined by MTT assay. Thoracic aortic rings were dissected from Sprague-Dawley rats. Contraction of aortic rings was induced by 60 mmol/L KCl or 10-6 mol/L phenylephrine （PE） in an ex-vivo perfusion system after BaP （100 tlmol/L） incubation for 6 h. [Ca^2＋]i was measured using Fluo-4/AM. For in-vivo treatment, rats were injected with BaP for 4 weeks （10 mg/kg, weekly, i.p.）. Results BaP （1-500 μm） did not significantly affect cell viability; S9-activated BaP stimulated cell proliferation. BaP did not affect the contractile function of endothelium-intact or -denuded aortic rings. BaP did not affect ATP-induced （[Ca2＋]i） increases in human umbilical vein endothelial cells. In BaP-treated rats, heart rate and the number of circulating inflammatory cells were not affected. Body weight decreased while blood pressure increased significantly. The maximum aortic contractile responses to PE and KCI and the maximum aortic relaxation response to acetylcholine were significantly decreased by 25.0%, 34.2%, and 10.4%, respectively. Conclusion These results suggest, in accordance with its DNA-damaging properties, that metabolic activation is a prerequisite for BaP-induced cardiovascular toxicity.展开更多
On September 29th, the 12th China （Changzhou） International Animation Art Festival co-hosted by the Ministry of Culture and the People＇s Government of Jiangsu Province was unveiled. 182 animation enterprises, exhib...On September 29th, the 12th China （Changzhou） International Animation Art Festival co-hosted by the Ministry of Culture and the People＇s Government of Jiangsu Province was unveiled. 182 animation enterprises, exhibition groups and institutions coming from 40 countries and regions made their appearances in this event. At the opening ceremony, 11 projects signed the contracts, which covered such fields as animation brand authorization, co-production of animation films, market proxy of products and crossover cooperation with the animation industry, which have generated 1 billion yuan.展开更多
The National Art Museum of China(NAMOC) recently opened an exhibition on its collection of extremely valuable artworks and historical archives in celebration of its 50th anniversary NAMOC is a state-level institution ...The National Art Museum of China(NAMOC) recently opened an exhibition on its collection of extremely valuable artworks and historical archives in celebration of its 50th anniversary NAMOC is a state-level institution of fine arts in China. Over the past five decades,NAMOC has made active endeavors and significant contribution to showcasing achievements of Chinese fine arts,accumulating the country’s resources in fine arts,serving the展开更多
Ntho-ling Monastery is located in Zanda County of Ngari in the western part of Tibetan Autonomous Region,about 60 kilometers west to the site of the ancient kingdom of Guge.In the Tibetan language,
文摘The "museum fever" has become a new fashion of Chinese social culture, and the cross-border integration and innovation of "muscum+" has become a new engine for promoting high-quality development.
文摘The Exhibition of Cuhural Rel- ics of Shang and Zhou Dynas- ties Unearthed from Hubei Prov-ince was recently on view at the National Museum o｜China in Beijing, This exhi- bition displayed 122 pieces/sets of cul- tural relics from the collection of Huhei Provincial Museum as well as other ten museums of Hubei province, showcas- ing the civilization of ancient China dat- ing from the early Shang Dynasty （1600 - 1046 BC）to the Warring States period （475 - 221 BC）.
基金Project (Nos. 30200333 and 30570840) supported by the NationalNatural Science Foundation of China
文摘Insulin-like growth factor binding-protein-7 （IGFBP7） was obtained from our previous colonic adenocarcinoma （CRC） and normal mucosa suppression subtraction hybridization （SSH） cDNA libraries. By RT-PCR and immunohistochemistry, we found that IGFBP7 was overexpressed in CRC tissue compared to normal tissue. However, our in vitro experiments performed in 10 CRC cell lines showed that IGFBP7 expressed only in SW480 and Caco2 cell lines, which implied an underlying reversible regulatory mechanism. Using methylation-specific PCR （MSP） and bisulfite sodium PCR （BSP）, we found that its expression was associated with DNA hypomethylation of exonl. This was further supported by the in vitro study which showed restored IGFBP7 expression after demethylation agent 5-aza-2＇-deoxycytidine treatment. Correlation analysis between IGFBP7 expression and prognosis indicated that overexpression of IGFBP7 in CRC tissue correlated with favourable survival. Investigation of the functional role of IGFBP7 through transfection studies showed that IGFBP7 protein could inhibit growth rate, decrease colony formation activity, and induce apoptosis in RKO and SW620 cells, suggesting it a potential tumor suppressor protein in colorectal carcinogenesis. In conclusion, our study clearly demonstrated that IGFBP7 plays a potential tumor suppressor role against colorectal carcinogenesis and its expression is associated with DNA hypomethylation of exon 1.
基金supported by National Natural Science Foundation of China,grant numbers30872140and81172692Zhejiang Provincial Natural Science Foundation,R2100555Ministry of Science and Technology,China,2009DFB30390
文摘Abstract Objective To evaluate the possible vascular effects of an environment carcinogen benzo（a）pyrene （BaP）. Methods The cytotoxicit of BaP and rat liver S9 （0.25 mg/mL）-activated BaP were examined by MTT assay. Thoracic aortic rings were dissected from Sprague-Dawley rats. Contraction of aortic rings was induced by 60 mmol/L KCl or 10-6 mol/L phenylephrine （PE） in an ex-vivo perfusion system after BaP （100 tlmol/L） incubation for 6 h. [Ca^2＋]i was measured using Fluo-4/AM. For in-vivo treatment, rats were injected with BaP for 4 weeks （10 mg/kg, weekly, i.p.）. Results BaP （1-500 μm） did not significantly affect cell viability; S9-activated BaP stimulated cell proliferation. BaP did not affect the contractile function of endothelium-intact or -denuded aortic rings. BaP did not affect ATP-induced （[Ca2＋]i） increases in human umbilical vein endothelial cells. In BaP-treated rats, heart rate and the number of circulating inflammatory cells were not affected. Body weight decreased while blood pressure increased significantly. The maximum aortic contractile responses to PE and KCI and the maximum aortic relaxation response to acetylcholine were significantly decreased by 25.0%, 34.2%, and 10.4%, respectively. Conclusion These results suggest, in accordance with its DNA-damaging properties, that metabolic activation is a prerequisite for BaP-induced cardiovascular toxicity.
文摘On September 29th, the 12th China （Changzhou） International Animation Art Festival co-hosted by the Ministry of Culture and the People＇s Government of Jiangsu Province was unveiled. 182 animation enterprises, exhibition groups and institutions coming from 40 countries and regions made their appearances in this event. At the opening ceremony, 11 projects signed the contracts, which covered such fields as animation brand authorization, co-production of animation films, market proxy of products and crossover cooperation with the animation industry, which have generated 1 billion yuan.
文摘The National Art Museum of China(NAMOC) recently opened an exhibition on its collection of extremely valuable artworks and historical archives in celebration of its 50th anniversary NAMOC is a state-level institution of fine arts in China. Over the past five decades,NAMOC has made active endeavors and significant contribution to showcasing achievements of Chinese fine arts,accumulating the country’s resources in fine arts,serving the
文摘Ntho-ling Monastery is located in Zanda County of Ngari in the western part of Tibetan Autonomous Region,about 60 kilometers west to the site of the ancient kingdom of Guge.In the Tibetan language,
