During yellow sea green tide outbreak, the thalli of Ulva prolifera drifted more than 350 km on the sea surface from off shore of Jiangsu Province to Qingdao city, where they were exposed to complex changes of local e...During yellow sea green tide outbreak, the thalli of Ulva prolifera drifted more than 350 km on the sea surface from off shore of Jiangsu Province to Qingdao city, where they were exposed to complex changes of local environments. The purpose of this study is to investigate the response of the thalli to diurnal changes of environments on the sea surface, and a sea surface environment simulator (SSES) was designed to simulate the natural environment of floating U . prolifera mat. A control experiment is designed in the laboratory, which was processed under suitable conditions (20°C for temperature, 72 μmol photons/(m 2 ·s) for light intensity and 30 for salinity), and an in-situ research was conducted to study the photosynthetic responses of floating U . prolifera to diurnal changes of environments. The results show that the photosynthetic efficiency of the thalli decreased gradually with time, and decreased rapidly after 14:00 local time (LT). After exposed to the environment on the sea surface for 6 h, the photosynthetic activity of the thalli decreased significantly. Furthermore, physiological-level and molecular-level experiments revealed that non-photochemical quenching (NPQ), cyclic electron flow (CEF) and energy redistribution between PSI and PSII all played an important role in the strong photosynthetic plasticity of U . prolifera . NPQ is the most important photoprotective responses to environmental changes before 12:00 LT. The results also confirmed that the CEF and energy redistribution between PSI and PSII are the main synergistic eff ects for the thalli to adapt to the environmental changes when the process NPQ cannot work. And the result can further reveal the reason why U . prolifera can adapt to the living condition of long distance drift on the sea surface. The findings of this research could provide a theoretical basis for explaining outbreaks of the green tide and instructing the management of the problem.展开更多
The present study was conducted to determine the ef fects of elevated pCO_2 on growth, photosynthesis, dark respiration and inorganic carbon acquisition in the marine microalga D unaliella salina. To accomplish this, ...The present study was conducted to determine the ef fects of elevated pCO_2 on growth, photosynthesis, dark respiration and inorganic carbon acquisition in the marine microalga D unaliella salina. To accomplish this, D. salina was incubated in semi-continuous cultures under present-day CO_2 levels(390 μatm, p HN BS : 8.10), predicted year 2100 CO_2 levels(1 000 μatm, p HN BS : 7.78) and predicted year 2300 CO_2 levels(2 000 μatm, p H NBS : 7.49). Elevated pCO_2 significantly enhanced photosynthesis(in terms of gross photosynthetic O_2 evolution, ef fective quantum yield(ΔF/F' m), photosynthetic efficiency( α), maximum relative electron transport rate(r ETRm ax) and ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco) activity) and dark respiration of D. salina, but had insignificant effects on growth. The photosynthetic O_2 evolution of D. salina was significantly inhibited by the inhibitors acetazolamide(AZ), ethoxyzolamide(EZ) and 4,4'-diisothiocyanostilbene-2,2′-disulfonate(DIDS), indicating that D. salina is capable of acquiring HCO ˉ 3 via extracellular carbonic anhydrase and anion-exchange proteins. Furthermore, the lower inhibition of the photosynthetic O2 evolution at high pCO_2 levels by AZ, EZ and DIDS and the decreased carbonic anhydrase showed that carbon concentrating mechanisms were down-regulated at high pCO_2. In conclusion, our results show that photosynthesis, dark respiration and CCMs will be af fected by the increased pCO_2/low p H conditions predicted for the future, but that the responses of D. salina to high pCO_2/low p H might be modulated by other environmental factors such as light, nutrients and temperature. Therefore, further studies are needed to determine the interactive eff ects of pCO_2, temperature, light and nutrients on marine microalgae.展开更多
基金Supported by the National Key R&D Program of China(No.2016YFC1402102)the National Natural Science Foundation of China(No.41706121)+4 种基金the Basic Applied Researching Projects of Qingdao(No.15-9-1-37-jch)the Fundamental Research Funds for the Central Universities(No.201513046)the China Postdoctoral Science Foundation(No.2013M531648)the Natural Science Foundation of Shandong Province(No.ZR2014DP008)the NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences(No.U1606404)
文摘During yellow sea green tide outbreak, the thalli of Ulva prolifera drifted more than 350 km on the sea surface from off shore of Jiangsu Province to Qingdao city, where they were exposed to complex changes of local environments. The purpose of this study is to investigate the response of the thalli to diurnal changes of environments on the sea surface, and a sea surface environment simulator (SSES) was designed to simulate the natural environment of floating U . prolifera mat. A control experiment is designed in the laboratory, which was processed under suitable conditions (20°C for temperature, 72 μmol photons/(m 2 ·s) for light intensity and 30 for salinity), and an in-situ research was conducted to study the photosynthetic responses of floating U . prolifera to diurnal changes of environments. The results show that the photosynthetic efficiency of the thalli decreased gradually with time, and decreased rapidly after 14:00 local time (LT). After exposed to the environment on the sea surface for 6 h, the photosynthetic activity of the thalli decreased significantly. Furthermore, physiological-level and molecular-level experiments revealed that non-photochemical quenching (NPQ), cyclic electron flow (CEF) and energy redistribution between PSI and PSII all played an important role in the strong photosynthetic plasticity of U . prolifera . NPQ is the most important photoprotective responses to environmental changes before 12:00 LT. The results also confirmed that the CEF and energy redistribution between PSI and PSII are the main synergistic eff ects for the thalli to adapt to the environmental changes when the process NPQ cannot work. And the result can further reveal the reason why U . prolifera can adapt to the living condition of long distance drift on the sea surface. The findings of this research could provide a theoretical basis for explaining outbreaks of the green tide and instructing the management of the problem.
基金Supported by the Joint Funds of the National Natural Science Foundation of China and the Marine Science Research Center of the People’s Government of Shandong Province(No.U1406403)the National Natural Science Foundation of China(No.41476091)
文摘The present study was conducted to determine the ef fects of elevated pCO_2 on growth, photosynthesis, dark respiration and inorganic carbon acquisition in the marine microalga D unaliella salina. To accomplish this, D. salina was incubated in semi-continuous cultures under present-day CO_2 levels(390 μatm, p HN BS : 8.10), predicted year 2100 CO_2 levels(1 000 μatm, p HN BS : 7.78) and predicted year 2300 CO_2 levels(2 000 μatm, p H NBS : 7.49). Elevated pCO_2 significantly enhanced photosynthesis(in terms of gross photosynthetic O_2 evolution, ef fective quantum yield(ΔF/F' m), photosynthetic efficiency( α), maximum relative electron transport rate(r ETRm ax) and ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco) activity) and dark respiration of D. salina, but had insignificant effects on growth. The photosynthetic O_2 evolution of D. salina was significantly inhibited by the inhibitors acetazolamide(AZ), ethoxyzolamide(EZ) and 4,4'-diisothiocyanostilbene-2,2′-disulfonate(DIDS), indicating that D. salina is capable of acquiring HCO ˉ 3 via extracellular carbonic anhydrase and anion-exchange proteins. Furthermore, the lower inhibition of the photosynthetic O2 evolution at high pCO_2 levels by AZ, EZ and DIDS and the decreased carbonic anhydrase showed that carbon concentrating mechanisms were down-regulated at high pCO_2. In conclusion, our results show that photosynthesis, dark respiration and CCMs will be af fected by the increased pCO_2/low p H conditions predicted for the future, but that the responses of D. salina to high pCO_2/low p H might be modulated by other environmental factors such as light, nutrients and temperature. Therefore, further studies are needed to determine the interactive eff ects of pCO_2, temperature, light and nutrients on marine microalgae.