The aim of this study is to isolate protoplasts from Undaria pinnatifida. Protoplasts of the alga were isolated enzymatically by using alginate lyase, which was prepared by fermenting culture of a strain Vibrio sp. 51...The aim of this study is to isolate protoplasts from Undaria pinnatifida. Protoplasts of the alga were isolated enzymatically by using alginate lyase, which was prepared by fermenting culture of a strain Vibrio sp. 510. Monofacterial method was applied for optimizing digestion condition. The optimum condition for protoplast preparation is enzymatic digestion at 28 ℃ for 2 h using alginate lyase at the concentration of 213.36 U (8 mL) every 0.5 g fresh thalline with NaCl 50 and at the shaking speed of 150 r min -1 during digestion. The protoplast yield can reach 2.62±0.09 million per 0.5 g fresh leave under the optimum condition. The enzyme activity is inhibited by Ca 2+ and slightly enhanced by Fe 2+ and Mn 2+ at concentrations of 0.05, 0.08 and 0.10 mol L -1.展开更多
Bicyclic Sesquiterpanes （BSs） are ubiquitous components of crude oils and ancient sediments. BSs in crude oils and diesel oil were identified and characterized, the effects of moderate weathering on BS distributions...Bicyclic Sesquiterpanes （BSs） are ubiquitous components of crude oils and ancient sediments. BSs in crude oils and diesel oil were identified and characterized, the effects of moderate weathering on BS distributions were discussed, and a methodology using diagnostic indices of BSs was developed for oil correlation and dif- ferentiation. The results showed that crude oils and oil products had different abundances and distributions of BSs and, consequently, resulted in different diagnostic ratios. The selected diagnostic ratio indices of BSs, such as BS4/BS5 （ratio of 4,4,8,9,9-pentamethyl-decahydronaphthalene to 8/3 （H）-drimane）, BS6/BS5 （ratio of 4,4,9,9,10-pentamethyl-decahydronaphthalene to 8/3 （H）-drimane）, BS8/BS9 （ratio of the second peak to the third peak of C16 sequiterpane）, and BS8/BSIO （ratio of C16 sequiterpane to 8fl （H）-homodrimane）, still maintained better stabilities （%RSD 〈 5%） after weathering for 30 d. The longer weathering process （150 d） had some effect on such ratios （5% 〈 %RSD 〈 10%）. The facts of the uniqueness, abundance in petroleum, and chemical stability of BSs enable them to be suitable as an effective diagnostic means for identifying spilled oil with moderate weathering, particularly for lighter refined product samples that are difficult to identify by current techniques.展开更多
文摘The aim of this study is to isolate protoplasts from Undaria pinnatifida. Protoplasts of the alga were isolated enzymatically by using alginate lyase, which was prepared by fermenting culture of a strain Vibrio sp. 510. Monofacterial method was applied for optimizing digestion condition. The optimum condition for protoplast preparation is enzymatic digestion at 28 ℃ for 2 h using alginate lyase at the concentration of 213.36 U (8 mL) every 0.5 g fresh thalline with NaCl 50 and at the shaking speed of 150 r min -1 during digestion. The protoplast yield can reach 2.62±0.09 million per 0.5 g fresh leave under the optimum condition. The enzyme activity is inhibited by Ca 2+ and slightly enhanced by Fe 2+ and Mn 2+ at concentrations of 0.05, 0.08 and 0.10 mol L -1.
基金Key Projects in the Yantai Science & Technology Pillar Program under contract No. 2011060the National Natural Science Foundation of China under contract Nos 40806048 and 41206089Hundred Talents Program of Chinese Academy of Sciences
文摘Bicyclic Sesquiterpanes （BSs） are ubiquitous components of crude oils and ancient sediments. BSs in crude oils and diesel oil were identified and characterized, the effects of moderate weathering on BS distributions were discussed, and a methodology using diagnostic indices of BSs was developed for oil correlation and dif- ferentiation. The results showed that crude oils and oil products had different abundances and distributions of BSs and, consequently, resulted in different diagnostic ratios. The selected diagnostic ratio indices of BSs, such as BS4/BS5 （ratio of 4,4,8,9,9-pentamethyl-decahydronaphthalene to 8/3 （H）-drimane）, BS6/BS5 （ratio of 4,4,9,9,10-pentamethyl-decahydronaphthalene to 8/3 （H）-drimane）, BS8/BS9 （ratio of the second peak to the third peak of C16 sequiterpane）, and BS8/BSIO （ratio of C16 sequiterpane to 8fl （H）-homodrimane）, still maintained better stabilities （%RSD 〈 5%） after weathering for 30 d. The longer weathering process （150 d） had some effect on such ratios （5% 〈 %RSD 〈 10%）. The facts of the uniqueness, abundance in petroleum, and chemical stability of BSs enable them to be suitable as an effective diagnostic means for identifying spilled oil with moderate weathering, particularly for lighter refined product samples that are difficult to identify by current techniques.
