目的运用Meta分析探寻SLC6A11基因rs2272394、rs2272395和rs2272400多态性是否与成人耐药性癫痫患者的耐药性密切相关。方法制订文献检索方案,通过检索Pubmed、Embase、Cochrane图书馆、Web of Science数据库、中国知网、万方数据知识...目的运用Meta分析探寻SLC6A11基因rs2272394、rs2272395和rs2272400多态性是否与成人耐药性癫痫患者的耐药性密切相关。方法制订文献检索方案,通过检索Pubmed、Embase、Cochrane图书馆、Web of Science数据库、中国知网、万方数据知识服务平台和维普资讯中文期刊服务平台检索数据库搜索有关SLC6A11基因多态性与耐药性癫痫治疗相关的中外文献,由两人共同筛选文献,提取数据并进行交叉核对,对纳入的文献进行质量评价后,将符合标准的文献纳入,使用R4.2.1软件对文献进行Meta分析,同时采用基因模型进行分析。结果4篇文献中的9项研究被纳入,其中耐药组病例共有1172例,药物敏感组病例共有1340例。其中等位基因C与T采用固定效应模型得出OR=0.89,95%CI=0.79~1.01;剔除一篇边缘性研究后,结果示OR=0.93,95%CI=0.82~1.06;隐性基因模型CC/TC+TT的OR=0.79,95%CI=0.67~0.92;显性基因模型TT/TC+CC的OR=0.99,95%CI=0.80~1.23;加性基因模型CC/TT的OR=0.86,95%CI=0.63~1.16;超显性基因模型TT+CC/TC的OR=0.83,95%CI=0.72~0.97。结论目前暂无明确证据证明SLC6A11基因的rs2272394、rs2272395和rs2272400多态性与耐药性癫痫有关。展开更多
Background Increased levels of interleukin-6 （IL-6） and C-reactive protein （CRP） have been reported in patients with venous thromboembolisms （VTE）. However, prospective studies did not confirm an association bet...Background Increased levels of interleukin-6 （IL-6） and C-reactive protein （CRP） have been reported in patients with venous thromboembolisms （VTE）. However, prospective studies did not confirm an association between IL-6, CRP and their polymorphism with the risk of VTE. Methods One hundred and forty patients （including 66 males and 74 females, mean age （55.55±17.11） years） and one hundred and sixty controls （including 74 males and 86 females, mean age （56.58±12.24） years） were involved. An enzyme linked immunosorbent assay （ELISA） method was used for detecting the serum levels of inflammatory factors IL-6 and CRP in both groups. Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was used for analyzing the distribution of polymorphisms at the -572C/G and -597G/A sites of the promoter of the IL-6 gene and at 1059G/C of the CRP gene. Results Serum levels of IL-6 and CRP were significantly higher in the VTE group than in the control group （P 〈0.05）. The frequencies of-572C/G promoter polymorphisms CC, CG, and GG in the IL-6 gene were found to be 34%, 48%, and 18%, respectively, and the derived allele frequencies for the C and G alleles were 58% and 42%. There was a significant difference in the -572C/G promoter polymorphisms between the VTE group and control group （P 〈0.05）. For the -597G/A polymorphism, individuals all carried the GG and GA type; AA genotypes were not detected. The frequency of the GG, GC, and CC genotypes at the CRP1059G/C promoter was 87.57%, 7.86% and 3.57% in V-rE group, while 86.25%, 10%, and 3.75% in control group, respectively. The frequency of G and C alleles at CRP 1059G/C was 91.43% and 8.57% in VTE group and 91.56% and 8.44% in the control group. The results showed that there was no statistically significant difference of 1059G/C genotype and mutation frequency of the allele between the VTE group and control group （P 〉0.05）. Multiple Logistic regression analysis showed CC homozygotes of the IL-6 -572G/C, body mass index （BMI）, and CRP, IL-6, and high-density lipoprotein cholesterol （HDL-C） were independent risk factors for VTE （P 〈0.05）. Conclusions We found that V-rE was associated with IL-6 and CRP levels, and there was an association of IL-6 and its promoter polymorphism at -572G/C with the risk of VTE. Thus far, a causal relationship between inflammation and VTE remains to be clarified and more prospective data are required.展开更多
Background It is of value to identify the non-invasive means that can accurately reflect the blood supply of epiphysis and is more sensitive in detection of early ischemia of epiphysis than the conventional gadoterid...Background It is of value to identify the non-invasive means that can accurately reflect the blood supply of epiphysis and is more sensitive in detection of early ischemia of epiphysis than the conventional gadoteridol （Gd）-enhanced SE TlWI. The aim of this study was to evaluate the blood supply of various anatomic regions at the end of normal growing long bone using dynamic Gd-enhanced MR imaging and compare the sensitivities between dynamic Gd-enhanced MR imaging and conventional Gd-enhanced SE TlWl in the detection of decreased blood perfusion of early epiphyseal ischemia. Methods Twenty-seven two-week-old piglets were used in this study. For the study of the end of normal growing long bone, unilateral MR imaging of the distal femur and proximal tibia was performed on eleven piglets. The comparison was made among various anatomic regions （physeal and epiphyseal cartilage, metaphyseal spongiosa, the secondary ossification center and metaphysis） using MRI in terms of the enhancement ratio and speed. Their relationships with the histological findings, including RBC/mm^2 and vessel distribution, were evaluated. To examine ischemic femoral head, 16 piglets were divided into two groups, with the control group having 8 piglets （involving 16 normal hips） and an ischemic group having 8 piglets （involving 16 hips with hyperabduction）. In the ischemic group, MR imaging was performed on the hips in the hyperabduction immobilized persistently for 30 minutes. After MRI, the piglets were allowed to ambulate freely for 1 day and the same MR scanning was then repeated in a neutral position. The difference in enhancement ratio and speed of the femoral head between the control and ischemic group were evaluated. Results With regard to the end of normal growing long bone, the enhancement ratio of the metaphyseal spongiosa was greatest among all the anatomic regions （P 〈0.001）. The enhancement ratio of physeal cartilage was greater than that of epiphyseal cartilage （P 〈0. 001）, which was the lowest in all tissues （P 〈0.001）. The enhancement speed of the spongiosa was greater than that of physis but the difference was not significant （P 〉0.05）. The enhancement speed of physis was greater than that of epiphyseal cartilage （P 〈0.05）, which was the lowest among all the tissues （P 〈0.05）. The enhancement ratio and speed were found to be related to the histological findings, including RBC/mm^2 （R 〉0.75） and distribution of vessels in the tissues. With ischemic femoral head, the enhancement ratios of physis, anterior part and posterior part of capital femoral epiphysis were significantly lower （P 〈0.05） and enhanced more slowly （P 〈0.05） than those of normal femoral head on dynamic Gd-enhanced MR imaging. On conventional Gd-enhanced SE TlWl, however, no apparent decrease in enhancement ratio and speed in ischemic hips was found （P 〈0.05）, when they were compared with those in the normal hips. Conclusions Dynamic gadoteridol-enhanced MR imaging can reveal the blood supply in various anatomic regions of the end of normal growing long bone. It is more sensitive than conventional Gd-enhanced SE TlWl in the detection of early epiphyseal ischemia.展开更多
文摘目的运用Meta分析探寻SLC6A11基因rs2272394、rs2272395和rs2272400多态性是否与成人耐药性癫痫患者的耐药性密切相关。方法制订文献检索方案,通过检索Pubmed、Embase、Cochrane图书馆、Web of Science数据库、中国知网、万方数据知识服务平台和维普资讯中文期刊服务平台检索数据库搜索有关SLC6A11基因多态性与耐药性癫痫治疗相关的中外文献,由两人共同筛选文献,提取数据并进行交叉核对,对纳入的文献进行质量评价后,将符合标准的文献纳入,使用R4.2.1软件对文献进行Meta分析,同时采用基因模型进行分析。结果4篇文献中的9项研究被纳入,其中耐药组病例共有1172例,药物敏感组病例共有1340例。其中等位基因C与T采用固定效应模型得出OR=0.89,95%CI=0.79~1.01;剔除一篇边缘性研究后,结果示OR=0.93,95%CI=0.82~1.06;隐性基因模型CC/TC+TT的OR=0.79,95%CI=0.67~0.92;显性基因模型TT/TC+CC的OR=0.99,95%CI=0.80~1.23;加性基因模型CC/TT的OR=0.86,95%CI=0.63~1.16;超显性基因模型TT+CC/TC的OR=0.83,95%CI=0.72~0.97。结论目前暂无明确证据证明SLC6A11基因的rs2272394、rs2272395和rs2272400多态性与耐药性癫痫有关。
文摘Background Increased levels of interleukin-6 （IL-6） and C-reactive protein （CRP） have been reported in patients with venous thromboembolisms （VTE）. However, prospective studies did not confirm an association between IL-6, CRP and their polymorphism with the risk of VTE. Methods One hundred and forty patients （including 66 males and 74 females, mean age （55.55±17.11） years） and one hundred and sixty controls （including 74 males and 86 females, mean age （56.58±12.24） years） were involved. An enzyme linked immunosorbent assay （ELISA） method was used for detecting the serum levels of inflammatory factors IL-6 and CRP in both groups. Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was used for analyzing the distribution of polymorphisms at the -572C/G and -597G/A sites of the promoter of the IL-6 gene and at 1059G/C of the CRP gene. Results Serum levels of IL-6 and CRP were significantly higher in the VTE group than in the control group （P 〈0.05）. The frequencies of-572C/G promoter polymorphisms CC, CG, and GG in the IL-6 gene were found to be 34%, 48%, and 18%, respectively, and the derived allele frequencies for the C and G alleles were 58% and 42%. There was a significant difference in the -572C/G promoter polymorphisms between the VTE group and control group （P 〈0.05）. For the -597G/A polymorphism, individuals all carried the GG and GA type; AA genotypes were not detected. The frequency of the GG, GC, and CC genotypes at the CRP1059G/C promoter was 87.57%, 7.86% and 3.57% in V-rE group, while 86.25%, 10%, and 3.75% in control group, respectively. The frequency of G and C alleles at CRP 1059G/C was 91.43% and 8.57% in VTE group and 91.56% and 8.44% in the control group. The results showed that there was no statistically significant difference of 1059G/C genotype and mutation frequency of the allele between the VTE group and control group （P 〉0.05）. Multiple Logistic regression analysis showed CC homozygotes of the IL-6 -572G/C, body mass index （BMI）, and CRP, IL-6, and high-density lipoprotein cholesterol （HDL-C） were independent risk factors for VTE （P 〈0.05）. Conclusions We found that V-rE was associated with IL-6 and CRP levels, and there was an association of IL-6 and its promoter polymorphism at -572G/C with the risk of VTE. Thus far, a causal relationship between inflammation and VTE remains to be clarified and more prospective data are required.
文摘Background It is of value to identify the non-invasive means that can accurately reflect the blood supply of epiphysis and is more sensitive in detection of early ischemia of epiphysis than the conventional gadoteridol （Gd）-enhanced SE TlWI. The aim of this study was to evaluate the blood supply of various anatomic regions at the end of normal growing long bone using dynamic Gd-enhanced MR imaging and compare the sensitivities between dynamic Gd-enhanced MR imaging and conventional Gd-enhanced SE TlWl in the detection of decreased blood perfusion of early epiphyseal ischemia. Methods Twenty-seven two-week-old piglets were used in this study. For the study of the end of normal growing long bone, unilateral MR imaging of the distal femur and proximal tibia was performed on eleven piglets. The comparison was made among various anatomic regions （physeal and epiphyseal cartilage, metaphyseal spongiosa, the secondary ossification center and metaphysis） using MRI in terms of the enhancement ratio and speed. Their relationships with the histological findings, including RBC/mm^2 and vessel distribution, were evaluated. To examine ischemic femoral head, 16 piglets were divided into two groups, with the control group having 8 piglets （involving 16 normal hips） and an ischemic group having 8 piglets （involving 16 hips with hyperabduction）. In the ischemic group, MR imaging was performed on the hips in the hyperabduction immobilized persistently for 30 minutes. After MRI, the piglets were allowed to ambulate freely for 1 day and the same MR scanning was then repeated in a neutral position. The difference in enhancement ratio and speed of the femoral head between the control and ischemic group were evaluated. Results With regard to the end of normal growing long bone, the enhancement ratio of the metaphyseal spongiosa was greatest among all the anatomic regions （P 〈0.001）. The enhancement ratio of physeal cartilage was greater than that of epiphyseal cartilage （P 〈0. 001）, which was the lowest in all tissues （P 〈0.001）. The enhancement speed of the spongiosa was greater than that of physis but the difference was not significant （P 〉0.05）. The enhancement speed of physis was greater than that of epiphyseal cartilage （P 〈0.05）, which was the lowest among all the tissues （P 〈0.05）. The enhancement ratio and speed were found to be related to the histological findings, including RBC/mm^2 （R 〉0.75） and distribution of vessels in the tissues. With ischemic femoral head, the enhancement ratios of physis, anterior part and posterior part of capital femoral epiphysis were significantly lower （P 〈0.05） and enhanced more slowly （P 〈0.05） than those of normal femoral head on dynamic Gd-enhanced MR imaging. On conventional Gd-enhanced SE TlWl, however, no apparent decrease in enhancement ratio and speed in ischemic hips was found （P 〈0.05）, when they were compared with those in the normal hips. Conclusions Dynamic gadoteridol-enhanced MR imaging can reveal the blood supply in various anatomic regions of the end of normal growing long bone. It is more sensitive than conventional Gd-enhanced SE TlWl in the detection of early epiphyseal ischemia.
