艺术理论学科的新发展——谈“艺术概论”教材建设

经过新中国60年的发展,我国的“艺术概论”教材建设取得了长足的进步。以此为依据可以认为,艺术学这一20世纪20年代便已引入国内的学科,目前正处在一个新的历史发展时期。无论从艺术观念、理论视野还是研究领域等诸多方面来看,“艺术概论”教材都有明显的突破和拓展。同时,当前的教材建设仍然存在一些需要改进的地方,比如教材的现实针对性、一般理论问题与具体艺术现象的联系问题等。仅以艺术分类问题为例,目前就存在划分依据不明确、划分方式易混乱等亟待解决的问题。随着学科建设的进一步发展,观念先进、体系合理、内容丰富的新教材必将不断涌现出来。

Expression, purification of a synthetic fuse-protein-TSF from PF4 and TSP1 fragments and its effect on angio-genesis and tumor growth

Sequences encoding PF4 (58-70) and TSP1 (429-459) were linked to yield a single gene TSF which encodes the fuse-protein of TSF. The gene was cloned into a pGEX-2T expression vector to generate a protein GST-TSF, which was strongly expressed in E. coli. The purified GST-TSF was degraded with thrombin to generate the protein TSF. With the methods of MTT and wound repair assay, the effects of TSF on the proliferation and migration of EC were detected, respectively. The results showed that TSF significantly suppressed BAEC proliferation and migration in a dose-dependent manner. The fuse protein GST-TSF, and the peptides PF4 (58-70) and TSP1 (429-459) also inhibited BAEC proliferation and migration, respectively, but their inhibition rates were not as high as TSF. Using the CAM assay, it was shown that TSF, GST-TSF, PF4 (58-70) and TSP1 (429-459) inhibited angiogenesis in chick CAM potentially, the effect of TSF was the highest. In vivo, the growth of Lewis lung carcinoma was potently inhibited by TSF