目的对一株具有抗菌活性的牡丹内生真菌MD76进行鉴定并对其次级代谢产物进行分离。方法采用形态学观察结合5. 8s r DNA ITS序列分析鉴定菌株MD76的种类;利用硅胶、凝胶柱层析等技术对该菌的大米发酵产物乙酸乙酯浸提物进行分离,并通过...目的对一株具有抗菌活性的牡丹内生真菌MD76进行鉴定并对其次级代谢产物进行分离。方法采用形态学观察结合5. 8s r DNA ITS序列分析鉴定菌株MD76的种类;利用硅胶、凝胶柱层析等技术对该菌的大米发酵产物乙酸乙酯浸提物进行分离,并通过波谱数据对分离化合物鉴定。结果鉴定菌株MD76为首都叶点霉Phyllosticta capitalensis,并从该菌发酵物中分离到7种化合物:苯甲酸(1)、对羟基苯甲酸(2)、对羟基苯丙酸(3)、芍药苷(4)、氧化芍药苷(5)、没食子酸(6)、芹菜素(7)。结论首都叶点霉P. capitalensis为首次从牡丹健康组织中分离获得,7种化合物均为首次从该种真菌中提取鉴定。展开更多
Background Acute kidney injury （AKI） has been recognized as a major healthcare problem affecting millions of patients worldwide.However,epidemiologic data concerning AKI in China are still lacking.The objectives of ...Background Acute kidney injury （AKI） has been recognized as a major healthcare problem affecting millions of patients worldwide.However,epidemiologic data concerning AKI in China are still lacking.The objectives of this study were to characterize AKI defined by RIFLE criteria,assess the association with hospital mortality,and evaluate the impact of AKI in the context of other risk factors.Methods This prospective multicenter observational study enrolled 3,063 consecutive patients from 1 July 2009 to 31 August 2009 in 22 ICUs across China's Mainland.We excluded patients who were admitted for less than 24 hours （n=1623）,younger than 18 years （n=127）,receiving chronic hemodialysis （n=29）,receiving renal transplantation （n=1） and unknown reasons （n=28）.There were 1255 patients in the final analysis.AKI was diagnosed and classified according to RIFLE criteria.Results There were 396 patients （31.6％） who had AKI,with RIFLE maximum class R,I,and F in 126 （10.0％）,91 （7.3％）,and 179 （14.3％） patients,respectively.Renal function deteriorated in 206 patients （16.4％）.In comparison with non AKI patients,patients in the risk class on ICU admission were more likely to progress to the injury class （odds ratio （OR） 3.564,95％ confidence interval （CI） 1.706-7.443,P =0.001],while patients in the risk class （OR 5.215,95％ CI 2.798-9.719,P ＜0.001） and injury class （OR 13.316,95％ CI 7.507-23.622,P ＜0.001） had a significantly higher probability of deteriorating into failure class.The adjusted hazard ratios for 90-day mortality were 1.884 for the risk group,3.401 for the injury group,and 5.306 for the failure group.Conclusions The prevalence of AKI was high among critically ill patients in Chinese ICUs.In comparison with non-AKI patients,patients with RIFLE class R or class I on ICU admission were more susceptibility to progression to class I or class F.The RIFLE criteria were robust and correlated well with clinical deterioration and mortality.展开更多
文摘目的对一株具有抗菌活性的牡丹内生真菌MD76进行鉴定并对其次级代谢产物进行分离。方法采用形态学观察结合5. 8s r DNA ITS序列分析鉴定菌株MD76的种类;利用硅胶、凝胶柱层析等技术对该菌的大米发酵产物乙酸乙酯浸提物进行分离,并通过波谱数据对分离化合物鉴定。结果鉴定菌株MD76为首都叶点霉Phyllosticta capitalensis,并从该菌发酵物中分离到7种化合物:苯甲酸(1)、对羟基苯甲酸(2)、对羟基苯丙酸(3)、芍药苷(4)、氧化芍药苷(5)、没食子酸(6)、芹菜素(7)。结论首都叶点霉P. capitalensis为首次从牡丹健康组织中分离获得,7种化合物均为首次从该种真菌中提取鉴定。
文摘Background Acute kidney injury （AKI） has been recognized as a major healthcare problem affecting millions of patients worldwide.However,epidemiologic data concerning AKI in China are still lacking.The objectives of this study were to characterize AKI defined by RIFLE criteria,assess the association with hospital mortality,and evaluate the impact of AKI in the context of other risk factors.Methods This prospective multicenter observational study enrolled 3,063 consecutive patients from 1 July 2009 to 31 August 2009 in 22 ICUs across China's Mainland.We excluded patients who were admitted for less than 24 hours （n=1623）,younger than 18 years （n=127）,receiving chronic hemodialysis （n=29）,receiving renal transplantation （n=1） and unknown reasons （n=28）.There were 1255 patients in the final analysis.AKI was diagnosed and classified according to RIFLE criteria.Results There were 396 patients （31.6％） who had AKI,with RIFLE maximum class R,I,and F in 126 （10.0％）,91 （7.3％）,and 179 （14.3％） patients,respectively.Renal function deteriorated in 206 patients （16.4％）.In comparison with non AKI patients,patients in the risk class on ICU admission were more likely to progress to the injury class （odds ratio （OR） 3.564,95％ confidence interval （CI） 1.706-7.443,P =0.001],while patients in the risk class （OR 5.215,95％ CI 2.798-9.719,P ＜0.001） and injury class （OR 13.316,95％ CI 7.507-23.622,P ＜0.001） had a significantly higher probability of deteriorating into failure class.The adjusted hazard ratios for 90-day mortality were 1.884 for the risk group,3.401 for the injury group,and 5.306 for the failure group.Conclusions The prevalence of AKI was high among critically ill patients in Chinese ICUs.In comparison with non-AKI patients,patients with RIFLE class R or class I on ICU admission were more susceptibility to progression to class I or class F.The RIFLE criteria were robust and correlated well with clinical deterioration and mortality.
