Rotifer community structure was investigated in Yangcheng Lake in 2008. Dominant species and species diversity indices were determined and QB/T was used to assess water quality. The annual average density and biomass ...Rotifer community structure was investigated in Yangcheng Lake in 2008. Dominant species and species diversity indices were determined and QB/T was used to assess water quality. The annual average density and biomass of the rotifers in Yangcheng Lake was 2 894＋ 1 006 indYL and 12.47± 10.28 mg/L, respectively. The highest densities were observed in the western portion of the lake in March, but the highest biomass occurred in inflowing creeks in September. Within a year of monitoring in Yangcheng Lake, 93 species were identified and the dominant species were found to be Polyarthra trigla, Brachionus angularis, Keratella eochlearis, Keratella valga, Brachionus calyciflorus, and Filinia major. Of the species recorded, 75 were pollution indicator species. Density and biomass exhibited significant positive correlations with water temperature （R=0,209, P=0.003; R=0.446, P=0.000）, but the peak density showed two lags in response to chl a. According to the Jaccard similarity index （S0, the greatest similarity among dominant species occurred between creeks and the eastern part of the lake. The annual average Shannon-Wiener diversity index H＇, Margalef richness index D and Pielou evenness index J were 1.96±0.34, 1.61±0.50 and 0.77±0.10, respectively. In all four areas of Yangcheng Lake, β- mesosaprobic species comprised the largest share of pollution indicator species. These data suggest that Yangcheng Lake is mesosaprobic.展开更多
为建立检测草鱼白介素10(Ci IL-10)的双抗体夹心ELISA方法,首先克隆Ci IL-10基因、构建与表达原核表达重组质粒p ET-32a-Ci IL-10,并对表达产物进行纯化,以获得重组Ci IL-10(r Ci IL-10)纯化蛋白。然后,以纯化的r Ci IL-10蛋白为免疫原...为建立检测草鱼白介素10(Ci IL-10)的双抗体夹心ELISA方法,首先克隆Ci IL-10基因、构建与表达原核表达重组质粒p ET-32a-Ci IL-10,并对表达产物进行纯化,以获得重组Ci IL-10(r Ci IL-10)纯化蛋白。然后,以纯化的r Ci IL-10蛋白为免疫原制备兔源及鼠源抗r Ci IL-10的多克隆抗体,并对其进行纯化与酶标记。最后,以纯化的鼠抗r Ci IL-10抗体为捕获抗体,r Ci IL-10纯化蛋白为夹心抗原,HRP标记的兔抗Ci IL-10纯化抗体为检测抗体,通过优化反应条件,建立双抗体夹心ELISA检测方法。结果显示,该方法的优化反应条件包括,捕获抗体的最佳包被浓度为20μg·m L^(-1),检测抗体的最佳稀释度为1∶3 200,最佳封闭条件是使用5%脱脂奶粉,37°C封闭1.5 h,样品反应时间为2h,以OD450值≥0.174作为阳性判定标准。该方法的板内及板间重复性变异系数小于10%,最低检测限量为24.29ng·m L^(-1),与草鱼白介素6、草鱼肿瘤坏死因子、小鼠白介素10、小鼠干扰素和小鼠单核细胞趋化蛋白均无交叉反应。结果表明,建立的双抗体夹心ELISA方法具有较好的特异性、重复性和灵敏度,可用于草鱼白介素10的快速检测。展开更多
基金Supported by the Scientific Special Fund of Commonweal Industry (Agriculture) of Finance Ministry (No. ayhyzx07-045)the Key Discipline Projects of Shanghai (No. S30701)the Doctor Startup Projects of Shanghai Ocean University
文摘Rotifer community structure was investigated in Yangcheng Lake in 2008. Dominant species and species diversity indices were determined and QB/T was used to assess water quality. The annual average density and biomass of the rotifers in Yangcheng Lake was 2 894＋ 1 006 indYL and 12.47± 10.28 mg/L, respectively. The highest densities were observed in the western portion of the lake in March, but the highest biomass occurred in inflowing creeks in September. Within a year of monitoring in Yangcheng Lake, 93 species were identified and the dominant species were found to be Polyarthra trigla, Brachionus angularis, Keratella eochlearis, Keratella valga, Brachionus calyciflorus, and Filinia major. Of the species recorded, 75 were pollution indicator species. Density and biomass exhibited significant positive correlations with water temperature （R=0,209, P=0.003; R=0.446, P=0.000）, but the peak density showed two lags in response to chl a. According to the Jaccard similarity index （S0, the greatest similarity among dominant species occurred between creeks and the eastern part of the lake. The annual average Shannon-Wiener diversity index H＇, Margalef richness index D and Pielou evenness index J were 1.96±0.34, 1.61±0.50 and 0.77±0.10, respectively. In all four areas of Yangcheng Lake, β- mesosaprobic species comprised the largest share of pollution indicator species. These data suggest that Yangcheng Lake is mesosaprobic.
文摘为建立检测草鱼白介素10(Ci IL-10)的双抗体夹心ELISA方法,首先克隆Ci IL-10基因、构建与表达原核表达重组质粒p ET-32a-Ci IL-10,并对表达产物进行纯化,以获得重组Ci IL-10(r Ci IL-10)纯化蛋白。然后,以纯化的r Ci IL-10蛋白为免疫原制备兔源及鼠源抗r Ci IL-10的多克隆抗体,并对其进行纯化与酶标记。最后,以纯化的鼠抗r Ci IL-10抗体为捕获抗体,r Ci IL-10纯化蛋白为夹心抗原,HRP标记的兔抗Ci IL-10纯化抗体为检测抗体,通过优化反应条件,建立双抗体夹心ELISA检测方法。结果显示,该方法的优化反应条件包括,捕获抗体的最佳包被浓度为20μg·m L^(-1),检测抗体的最佳稀释度为1∶3 200,最佳封闭条件是使用5%脱脂奶粉,37°C封闭1.5 h,样品反应时间为2h,以OD450值≥0.174作为阳性判定标准。该方法的板内及板间重复性变异系数小于10%,最低检测限量为24.29ng·m L^(-1),与草鱼白介素6、草鱼肿瘤坏死因子、小鼠白介素10、小鼠干扰素和小鼠单核细胞趋化蛋白均无交叉反应。结果表明,建立的双抗体夹心ELISA方法具有较好的特异性、重复性和灵敏度,可用于草鱼白介素10的快速检测。
