Fruit yield is the most important horticultural trait of tomato.SIMBP21,a SEPALLATA subclass MADS-box gene has been reported to have functions in regulating pedicel abscission zone identity and development and control...Fruit yield is the most important horticultural trait of tomato.SIMBP21,a SEPALLATA subclass MADS-box gene has been reported to have functions in regulating pedicel abscission zone identity and development and controlling sepal size in tomato.However,we generated transgenic tomato plants which overexpress SIMBP21 and found the transformants displayed curly leaves,abnormally shaped flowers with twisted and opened stamens,reduced yield parameters,and small and light seeds.Our studies on the gain-of-function phenotype and gene expression level showed that its novel aspects played important roles in determining leaf morphology,flower and inflorescence architecture,and seed size,as well as the fruit yield.Overexpression of SIMBP21 in tomato resulted in curly leaves with fewer leaflets due to the regulation of the critical leaf polarity genes that cause an imbalance between the midvein adaxial-abaxial cell growth.Defects in the architecture of flowers and inflorescences resulted in reduced fruit set.Furthermore,we demonstrated that SIMBP21 plays its role through inhibiting the expression of the genes involved in the determination of seed development in tomato and SIMBP21 protein can interact with other MADS-box protein(SIAGL11,TAGL1 and SIMBP3)to control seed size.Thus,these results suggest that overexpression of SIMBP21 causes multiple types of damage to plant growth and development,especially fruit yield,in tomato.展开更多
The purpose of this study is to explore the influence of co-suppressing tomato ACC oxidase Ⅰ on the expression of fruit ripening-related and pathogenesis-related protein genes, and on the biosynthesis of endogenous e...The purpose of this study is to explore the influence of co-suppressing tomato ACC oxidase Ⅰ on the expression of fruit ripening-related and pathogenesis-related protein genes, and on the biosynthesis of endogenous ethylene and storage ability of fruits. Specific fragments of several fruit ripening-related and pathogenesis-related protein genes from tomato （Lycopersicon esculentum） were cloned, such as the l-aminocyclopropane-1-carboxylic acid oxidase 1 gene （LeAC01）, 1- aminocyclopropane-l-carboxylic acid oxidase 3 gene （LeAC03）, EIN3-binding F-box 1 gene （LeEBF1）, pathogenesis-related protein 1 gene （LePR1）, pathogenesis-related protein 5 gene （LePR5）, and pathogenesis-related protein osmotin precursor gene （LeNP24） by PCR or RT-PCR. Then these specific DNA fragments were used as probes to hybridize with the total RNAs extracted from the wild type tomato Ailsa Craig （AC＋＋） and the LeAC01 co-suppression tomatoes （V1187 and T4B）, respectively. At the same time, ethylene production measurement and storage experiment of tomato fruits were carded out. The hybridization results indicated that the expression of fruit ripening-related genes such as LeACO3 and LeEBF1, and pathogenesis-related protein genes such as LePR1, LePR5, and LeNP24, were reduced sharply, and the ethylene production in the fruits, wounded leaves decreased and the storage time of ripening fruits was prolonged, when the expression of LeACO1 gene in the transgenic tomato was suppressed. In the co-suppression tomatoes, the expression of fruit ripening-related and pathogenesis-related protein genes were restrained at different degrees, the biosynthesis of endogenous ethylene decreased and the storage ability of tomato fruits increased.展开更多
基金the Training Program of Chongqing University Bioengineering College,China(0221001105301)the National Natural Science Foundation of China(31872121 and 31801870).
文摘Fruit yield is the most important horticultural trait of tomato.SIMBP21,a SEPALLATA subclass MADS-box gene has been reported to have functions in regulating pedicel abscission zone identity and development and controlling sepal size in tomato.However,we generated transgenic tomato plants which overexpress SIMBP21 and found the transformants displayed curly leaves,abnormally shaped flowers with twisted and opened stamens,reduced yield parameters,and small and light seeds.Our studies on the gain-of-function phenotype and gene expression level showed that its novel aspects played important roles in determining leaf morphology,flower and inflorescence architecture,and seed size,as well as the fruit yield.Overexpression of SIMBP21 in tomato resulted in curly leaves with fewer leaflets due to the regulation of the critical leaf polarity genes that cause an imbalance between the midvein adaxial-abaxial cell growth.Defects in the architecture of flowers and inflorescences resulted in reduced fruit set.Furthermore,we demonstrated that SIMBP21 plays its role through inhibiting the expression of the genes involved in the determination of seed development in tomato and SIMBP21 protein can interact with other MADS-box protein(SIAGL11,TAGL1 and SIMBP3)to control seed size.Thus,these results suggest that overexpression of SIMBP21 causes multiple types of damage to plant growth and development,especially fruit yield,in tomato.
基金supported by National Natural Science Foundation of China(30471180)Nature Science Foundation of Chongqing City,China(8045,2004-56).
文摘The purpose of this study is to explore the influence of co-suppressing tomato ACC oxidase Ⅰ on the expression of fruit ripening-related and pathogenesis-related protein genes, and on the biosynthesis of endogenous ethylene and storage ability of fruits. Specific fragments of several fruit ripening-related and pathogenesis-related protein genes from tomato （Lycopersicon esculentum） were cloned, such as the l-aminocyclopropane-1-carboxylic acid oxidase 1 gene （LeAC01）, 1- aminocyclopropane-l-carboxylic acid oxidase 3 gene （LeAC03）, EIN3-binding F-box 1 gene （LeEBF1）, pathogenesis-related protein 1 gene （LePR1）, pathogenesis-related protein 5 gene （LePR5）, and pathogenesis-related protein osmotin precursor gene （LeNP24） by PCR or RT-PCR. Then these specific DNA fragments were used as probes to hybridize with the total RNAs extracted from the wild type tomato Ailsa Craig （AC＋＋） and the LeAC01 co-suppression tomatoes （V1187 and T4B）, respectively. At the same time, ethylene production measurement and storage experiment of tomato fruits were carded out. The hybridization results indicated that the expression of fruit ripening-related genes such as LeACO3 and LeEBF1, and pathogenesis-related protein genes such as LePR1, LePR5, and LeNP24, were reduced sharply, and the ethylene production in the fruits, wounded leaves decreased and the storage time of ripening fruits was prolonged, when the expression of LeACO1 gene in the transgenic tomato was suppressed. In the co-suppression tomatoes, the expression of fruit ripening-related and pathogenesis-related protein genes were restrained at different degrees, the biosynthesis of endogenous ethylene decreased and the storage ability of tomato fruits increased.
