Genetically improved transgenic fish possess many beneficial economic traits; however, the commer-cial aquaculture of transgenic fish has not been performed till date. One of the major reasons for this is the possible...Genetically improved transgenic fish possess many beneficial economic traits; however, the commer-cial aquaculture of transgenic fish has not been performed till date. One of the major reasons for this is the possible ecological risk associated with the escape or release of the transgenic fish. Using a growth hormone transgenic fish with rapid growth characteristics as a subject, this paper analyzes the following: the essence of the potential ecological risks posed by transgenic fish; ecological risk in the current situation due to transgenic fish via one-factor phenotypic and fitness analysis, and mathe-matical model deduction. Then, it expounds new ideas and the latest findings using an artificially simulated ecosystem for the evaluation of the ecological risks posed by transgenic fish. Further, the study comments on the strategies and principles of controlling these ecological risks by using a trip-loid approach. Based on these results, we propose that ecological risk evaluation and prevention strategies are indispensable important components and should be accompanied with breeding re-search in order to provide enlightments for transgenic fish breeding, evaluation of the ecological risks posed by transgenic fish, and development of containment strategies against the risks.展开更多
The gonad is an essential organ for gen- erating sperm and ova in vertebrates. This review describes several pilot studies on gonad gene manipulation and development in fish. With antisense RNA techniques, we suppress...The gonad is an essential organ for gen- erating sperm and ova in vertebrates. This review describes several pilot studies on gonad gene manipulation and development in fish. With antisense RNA techniques, we suppressed the gonad devel- opment, and thus the fertility, of an antisense gonad- otropin-releasing hormone (sGnRH) transgenic common carp. Then, using a tissue-specific exoge- nous gene excision strategy with sexual compensa- tion, we knocked out the gonad-specific transgene. Under the control of the rainbow trout protamine promoter, the transgenic fish expressed the reporter gene eGFP specifically in the spermary. These re- sults indicate that the fish gonad is a new model or- gan that can improve contemporary biotechnology experiments. Herein we discuss the potential of fish gonad manipulation for resolving important biosafety problems regarding transgenic fish generation and producing the new transgenic animal bioreactor.展开更多
基金Supported by the Development Plan of the State Key Fundamental Research of China (Grant Nos. 2007CB109205 and 2007CB109206)the National Natural Sci-ence Foundation of China (Grant No. 30430540)the ‘863’ High Technology Project (Grant No. 2006AA10Z141)
文摘Genetically improved transgenic fish possess many beneficial economic traits; however, the commer-cial aquaculture of transgenic fish has not been performed till date. One of the major reasons for this is the possible ecological risk associated with the escape or release of the transgenic fish. Using a growth hormone transgenic fish with rapid growth characteristics as a subject, this paper analyzes the following: the essence of the potential ecological risks posed by transgenic fish; ecological risk in the current situation due to transgenic fish via one-factor phenotypic and fitness analysis, and mathe-matical model deduction. Then, it expounds new ideas and the latest findings using an artificially simulated ecosystem for the evaluation of the ecological risks posed by transgenic fish. Further, the study comments on the strategies and principles of controlling these ecological risks by using a trip-loid approach. Based on these results, we propose that ecological risk evaluation and prevention strategies are indispensable important components and should be accompanied with breeding re-search in order to provide enlightments for transgenic fish breeding, evaluation of the ecological risks posed by transgenic fish, and development of containment strategies against the risks.
基金supported by the National Natural Science Foundation of China(Grant No.30430540&30130050)the"863"High Technology Project(Grant No.2004AA213121)the Development Plan of the State Key Fundamental Research of China(Grant No.2001 CB109006).
文摘The gonad is an essential organ for gen- erating sperm and ova in vertebrates. This review describes several pilot studies on gonad gene manipulation and development in fish. With antisense RNA techniques, we suppressed the gonad devel- opment, and thus the fertility, of an antisense gonad- otropin-releasing hormone (sGnRH) transgenic common carp. Then, using a tissue-specific exoge- nous gene excision strategy with sexual compensa- tion, we knocked out the gonad-specific transgene. Under the control of the rainbow trout protamine promoter, the transgenic fish expressed the reporter gene eGFP specifically in the spermary. These re- sults indicate that the fish gonad is a new model or- gan that can improve contemporary biotechnology experiments. Herein we discuss the potential of fish gonad manipulation for resolving important biosafety problems regarding transgenic fish generation and producing the new transgenic animal bioreactor.
