Background:Atherosclerosis is a chronic cardiovascular disease of great concern.However,it is difficult to establish a direct connection between conventional small animal models and clinical practice.The pig's gen...Background:Atherosclerosis is a chronic cardiovascular disease of great concern.However,it is difficult to establish a direct connection between conventional small animal models and clinical practice.The pig's genome,physiology,and anatomy reflect human biology better than other laboratory animals,which is crucial for studying the pathogenesis of atherosclerosis.Methods:We used whole-genome sequencing data from nine Bama minipigs to perform a genome-wide linkage analysis,and further used bioinformatic tools to filter and identify underlying candidate genes.Candidate gene function prediction was performed using the online prediction tool STRING 12.0.Immunohistochemistry and immunofluorescence were used to detect the expression of proteins encoded by candidate genes.Results:We mapped differential single nucleotide polymorphisms(SNPs)to genes and obtained a total of 102 differential genes,then we used GO and KEGG pathway enrichment analysis to identify four candidate genes,including SLA-1,SLA-2,SLA-3,and TAP2.nsSNPs cause changes in the primary and tertiary structures of SLA-I and TAP2 proteins,the primary structures of these two proteins have undergone amino acid changes,and the tertiary structures also show slight changes.In addition,immunohistochemistry and immunofluorescence results showed that the expression changes of TAP2 protein in coronary arteries showed a trend of increasing from the middle layer to the inner layer.Conclusions:We have identified SLA-I and TAP2 as potential susceptibility genes of atherosclerosis,highlighting the importance of antigen processing and immune response in atherogenesis.展开更多
BACKGROUND The incidence of colorectal cancer(CRC)is increasing annually.Laparoscopic radical resection of CRC is a minimally invasive procedure preferred in clinical practice.AIM To investigate the clinical effect of...BACKGROUND The incidence of colorectal cancer(CRC)is increasing annually.Laparoscopic radical resection of CRC is a minimally invasive procedure preferred in clinical practice.AIM To investigate the clinical effect of laparoscopic radical resection of CRC on the basis of propensity score matching(PSM).METHODS The clinical data of 100 patients who received inpatient treatment for CRC at Changde Hospital,Xiangya School of Medicine,Central South University(The First People’s Hospital of Changde City)were analyzed retrospectively.The control group included patients who underwent open surgery(n=43),and those who underwent laparoscopic surgery formed the observation group(n=57).The baseline information of both groups was equipoised using 1×1 PSM.Differences in the perioperative parameters,inflammatory response,immune function,degree of pain,and physical status between the groups were analyzed.RESULTS Thirty patients from both groups were successfully matched.After PSM,baseline data showed no statistically significant differences between the groups:(1)Periop-erative parameters:The observation group had a longer surgery time,less intra-operative blood loss,earlier first ambulation and first anal exhaust times,and shorter gastric tube indwelling time than the control group;(2)Inflammatory response:24 h after surgery,the levels of interleukin-6(IL-6),C-reactive protein(CRP),and tumor necrosis factor-α(TNF-α)between groups were higher than preoperatively.IL-6,CRP,and TNF-αlevels in the observation group were lower than in the control group;(3)Immune function:At 24 h after surgery,counts of CD4-positive T-lymphocytes(CD4+)and CD4+/CD8-positive T-lymphocytes(CD8+)in both groups were lower than those before surgery,whereas CD8+was higher than that before surgery.At 24 h after surgery,both CD4+counts and CD4+/CD8+in the observation group were higher than those in the control group,whereas CD8+counts were lower;(4)Degree of pain:The visual analog scale scores in the observation group were lower than those in the control group at 24 and 72 h after surgery;and(5)Physical status:One month after surgery,the Karnofsky performance score in the observation group was higher than that in the control group.CONCLUSION Laparoscopic radical resection of CRC has significant benefits,such as reducing postoperative pain and postoperative inflammatory response,avoiding excessive immune inhibition,and contributing to postoperative recovery.展开更多
Spinal cord injury is a challenge in orthopedics because it causes irreversible damage to the central nervous system.Therefore,early treatment to prevent lesion expansion is crucial for the management of patients with...Spinal cord injury is a challenge in orthopedics because it causes irreversible damage to the central nervous system.Therefore,early treatment to prevent lesion expansion is crucial for the management of patients with spinal cord injury.Bexarotene,a type of retinoid,exerts therapeutic effects on patients with cutaneous T-cell lymphoma and Parkinson's disease.Bexarotene has been proven to promote autophagy,but it has not been used in the treatment of spinal cord injury.To investigate the effects of bexarotene on spinal cord injury,we established a mouse model of T11–T12 spinal cord contusion and performed daily intraperitoneal injection of bexarotene for 5 consecutive days.We found that bexarotene effectively reduced the deposition of collagen and the number of pathological neurons in the injured spinal cord,increased the number of synapses of nerve cells,reduced oxidative stress,inhibited pyroptosis,promoted the recovery of motor function,and reduced death.Inhibition of autophagy with 3-methyladenine reversed the effects of bexarotene on spinal cord injury.Bexarotene enhanced the nuclear translocation of transcription factor E3,which further activated AMP-activated protein kinase-S-phase kinase-associated protein 2-coactivator-associated arginine methyltransferase 1 and AMP-activated protein kinase-mammalian target of rapamycin signaling pathways.Intravenous injection of transcription factor E3 sh RNA or intraperitoneal injection of compound C,an AMP-activated protein kinase blocker,inhibited the effects of bexarotene.These findings suggest that bexarotene regulates nuclear translocation of transcription factor E3 through the AMP-activated protein kinase-Sphase kinase-associated protein 2-coactivator-associated arginine methyltransferase 1 and AMP-activated protein kinase-mammalian target of rapamycin signal pathways,promotes autophagy,decreases reactive oxygen species level,inhibits pyroptosis,and improves motor function after spinal cord injury.展开更多
The study of inter-system bias(ISB)is important for multi-system fusion and the performance of different signal compatibility.In this paper,the stability of ISB at the BDS3/BDS2 receiver end is calculated and analyzed...The study of inter-system bias(ISB)is important for multi-system fusion and the performance of different signal compatibility.In this paper,the stability of ISB at the BDS3/BDS2 receiver end is calculated and analyzed for different time spans(DOY 060~090 in 2021)from a total of 31 MGEX and iGMAS stations.We adopted two estimation strategies,random walk and constant approach,using the precision products of orbit and clock bias provided by WUM,the influence of which on ISB was also analyzed.Our results showed that the ISB value varied little within a day,and the mean of daily ISB standard deviation was only 0.037 m when the observation condition was good.The signal reception was continuous,indicating a high ISB stability for one day.If extending the time series to one month,however,the ISB standard deviation calculated by constant approach,in which a constant ISB is estimated on a daily basis was about 0.1 m,and the results of adjacent days were not continuous,with no apparent pattern.Concerning the random walk approach,the obtained ISB time series also had a jump,and the conclusion was the same as that of the constant strategy.Besides,receiver types showed a strong regularity in ISB numerical situation,and the distribution of ISB values corresponding to the same receiver type was relatively close.Therefore,we conclude that the ISB parameters remain stable in the short term(one day)and less stable in the long-term period.It is recommended that the ISB term should be set as a constant estimate every day in BDS3/BDS2 solutions,regardless of receiver type consistency.展开更多
Northeast China(NEC)is China’s national grain production base,and the local precipitation is vital for agriculture during the springtime.Therefore,understanding the dynamic origins of the NEC spring rainfall(NECSR)va...Northeast China(NEC)is China’s national grain production base,and the local precipitation is vital for agriculture during the springtime.Therefore,understanding the dynamic origins of the NEC spring rainfall(NECSR)variability is of socioeconomic importance.This study reveals an interdecadal change in the atmospheric teleconnections associated with the NECSR during a recent 60-year period(1961-2020).Before the mid-1980s,NECSR had been related to a Rossby wave train that is coupled with extratropical North Atlantic sea surface temperature(SST),whereas,since the mid-1980s,NECSR has been linked to a quite different Rossby wave train that is coupled with tropical North Atlantic SST.Both Rossby wave trains could lead to enhanced NECSR through anomalous cyclones over East Asia.The weakening of the westerly jet over North America is found to be mainly responsible for the alternation of the atmospheric teleconnections associated with NECSR during two epochs.展开更多
Background:Hundreds of single-nucleotide polymorphism(SNP)sites have been found to be potential genetic markers of type 2 diabetes mellitus(T2DM).However,SNPs related to T2DM in minipigs have been less reported.This s...Background:Hundreds of single-nucleotide polymorphism(SNP)sites have been found to be potential genetic markers of type 2 diabetes mellitus(T2DM).However,SNPs related to T2DM in minipigs have been less reported.This study aimed to screen the T2DM-susceptible candidate SNP loci in Bama minipigs so as to improve the success rate of the minipig T2DM model.Methods:The genomic DNAs of three Bama minipigs with T2DM,six sibling lowsusceptibility minipigs with T2DM,and three normal control minipigs were compared by whole-genome sequencing.The T2DM Bama minipig-specific loci were obtained,and their functions were annotated.Meanwhile,the Biomart software was used to perform homology alignment with T2DM-related loci obtained from the human genome-wide association study to screen candidate SNP markers for T2DM in Bama miniature pigs.Results:Whole-genome resequencing detected 6960 specific loci in the minipigs with T2DM,and 13 loci corresponding to 9 diabetes-related genes were selected.Further,a set of 122 specific loci in 69 orthologous genes of human T2DM candidate genes were obtained in the pigs.Collectively,a batch of T2DM-susceptible candidate SNP markers in Bama minipigs,covering 16 genes and 135 loci,was established.Conclusions:Whole-genome sequencing and comparative genomics analysis of the orthologous genes in pigs that corresponded to the human T2DM-related variant loci successfully screened out T2DM-susceptible candidate markers in Bama miniature pigs.Using these loci to predict the susceptibility of the pigs before constructing an animal model of T2DM may help to establish an ideal animal model.展开更多
Quantum heat transport is considered as an indispensable branch of quantum thermodynamics to potentially improve performance of thermodynamic devices.We theoretically propose a dissipative qubit-photon system composed...Quantum heat transport is considered as an indispensable branch of quantum thermodynamics to potentially improve performance of thermodynamic devices.We theoretically propose a dissipative qubit-photon system composed of multiple coupled resonators interacting with a single two-level qubit,to explore the steady-state heat transport by tuning both the inter-resonator photon hopping and the qubit-photon coupling.Specifically in the three-mode case,the dramatic enhancement and suppression of the heat current into the central resonator can be modulated by the corresponding frequency,compared to the currents into two edge resonators.Moreover,fruitful cycle current components are unraveled at weak qubit-photon coupling,which are crucial to exhibit the nonmonotonic feature with increase of the reservoir temperature bias.In the one-dimensional case under the mean-field framework,the influence of the photon hopping on heat transport is analyzed.The steady-state heat current is comparatively enhanced to the single-mode limit at weak qubit-photon coupling,stemming from the nonvanishing mean-field photon excitation parameter and the additional cycle current component.We hope these obtained results may have possible applications in quantum thermodynamic manipulation and energy harvesting.展开更多
Link prediction,also known as Knowledge Graph Completion(KGC),is the common task in Knowledge Graphs(KGs)to predict missing connections between entities.Most existing methods focus on designing shallow,scalable models...Link prediction,also known as Knowledge Graph Completion(KGC),is the common task in Knowledge Graphs(KGs)to predict missing connections between entities.Most existing methods focus on designing shallow,scalable models,which have less expressive than deep,multi-layer models.Furthermore,most operations like addition,matrix multiplications or factorization are handcrafted based on a few known relation patterns in several wellknown datasets,such as FB15k,WN18,etc.However,due to the diversity and complex nature of real-world data distribution,it is inherently difficult to preset all latent patterns.To address this issue,we proposeKGE-ANS,a novel knowledge graph embedding framework for general link prediction tasks using automatic network search.KGEANS can learn a deep,multi-layer effective architecture to adapt to different datasets through neural architecture search.In addition,the general search spacewe designed is tailored forKGtasks.We performextensive experiments on benchmark datasets and the dataset constructed in this paper.The results show that our KGE-ANS outperforms several state-of-the-art methods,especially on these datasets with complex relation patterns.展开更多
基金supported by the Special Scientific Research Project of Army Laboratory Animals(No.SYDW[2020]01)National Natural Science Foundation of ChinaNo.32370568。
文摘Background:Atherosclerosis is a chronic cardiovascular disease of great concern.However,it is difficult to establish a direct connection between conventional small animal models and clinical practice.The pig's genome,physiology,and anatomy reflect human biology better than other laboratory animals,which is crucial for studying the pathogenesis of atherosclerosis.Methods:We used whole-genome sequencing data from nine Bama minipigs to perform a genome-wide linkage analysis,and further used bioinformatic tools to filter and identify underlying candidate genes.Candidate gene function prediction was performed using the online prediction tool STRING 12.0.Immunohistochemistry and immunofluorescence were used to detect the expression of proteins encoded by candidate genes.Results:We mapped differential single nucleotide polymorphisms(SNPs)to genes and obtained a total of 102 differential genes,then we used GO and KEGG pathway enrichment analysis to identify four candidate genes,including SLA-1,SLA-2,SLA-3,and TAP2.nsSNPs cause changes in the primary and tertiary structures of SLA-I and TAP2 proteins,the primary structures of these two proteins have undergone amino acid changes,and the tertiary structures also show slight changes.In addition,immunohistochemistry and immunofluorescence results showed that the expression changes of TAP2 protein in coronary arteries showed a trend of increasing from the middle layer to the inner layer.Conclusions:We have identified SLA-I and TAP2 as potential susceptibility genes of atherosclerosis,highlighting the importance of antigen processing and immune response in atherogenesis.
基金Supported by Scientific Research Project of Hunan Provincial Health Commission,No.202204114103.
文摘BACKGROUND The incidence of colorectal cancer(CRC)is increasing annually.Laparoscopic radical resection of CRC is a minimally invasive procedure preferred in clinical practice.AIM To investigate the clinical effect of laparoscopic radical resection of CRC on the basis of propensity score matching(PSM).METHODS The clinical data of 100 patients who received inpatient treatment for CRC at Changde Hospital,Xiangya School of Medicine,Central South University(The First People’s Hospital of Changde City)were analyzed retrospectively.The control group included patients who underwent open surgery(n=43),and those who underwent laparoscopic surgery formed the observation group(n=57).The baseline information of both groups was equipoised using 1×1 PSM.Differences in the perioperative parameters,inflammatory response,immune function,degree of pain,and physical status between the groups were analyzed.RESULTS Thirty patients from both groups were successfully matched.After PSM,baseline data showed no statistically significant differences between the groups:(1)Periop-erative parameters:The observation group had a longer surgery time,less intra-operative blood loss,earlier first ambulation and first anal exhaust times,and shorter gastric tube indwelling time than the control group;(2)Inflammatory response:24 h after surgery,the levels of interleukin-6(IL-6),C-reactive protein(CRP),and tumor necrosis factor-α(TNF-α)between groups were higher than preoperatively.IL-6,CRP,and TNF-αlevels in the observation group were lower than in the control group;(3)Immune function:At 24 h after surgery,counts of CD4-positive T-lymphocytes(CD4+)and CD4+/CD8-positive T-lymphocytes(CD8+)in both groups were lower than those before surgery,whereas CD8+was higher than that before surgery.At 24 h after surgery,both CD4+counts and CD4+/CD8+in the observation group were higher than those in the control group,whereas CD8+counts were lower;(4)Degree of pain:The visual analog scale scores in the observation group were lower than those in the control group at 24 and 72 h after surgery;and(5)Physical status:One month after surgery,the Karnofsky performance score in the observation group was higher than that in the control group.CONCLUSION Laparoscopic radical resection of CRC has significant benefits,such as reducing postoperative pain and postoperative inflammatory response,avoiding excessive immune inhibition,and contributing to postoperative recovery.
基金grants from Zhejiang Provincial Medicine and Health Technology Project,No.2021KY214(to SS)Zhejiang Provincial Science and Technology Project of Traditional Chinese Medicine,No.2021ZB183(to HX)。
文摘Spinal cord injury is a challenge in orthopedics because it causes irreversible damage to the central nervous system.Therefore,early treatment to prevent lesion expansion is crucial for the management of patients with spinal cord injury.Bexarotene,a type of retinoid,exerts therapeutic effects on patients with cutaneous T-cell lymphoma and Parkinson's disease.Bexarotene has been proven to promote autophagy,but it has not been used in the treatment of spinal cord injury.To investigate the effects of bexarotene on spinal cord injury,we established a mouse model of T11–T12 spinal cord contusion and performed daily intraperitoneal injection of bexarotene for 5 consecutive days.We found that bexarotene effectively reduced the deposition of collagen and the number of pathological neurons in the injured spinal cord,increased the number of synapses of nerve cells,reduced oxidative stress,inhibited pyroptosis,promoted the recovery of motor function,and reduced death.Inhibition of autophagy with 3-methyladenine reversed the effects of bexarotene on spinal cord injury.Bexarotene enhanced the nuclear translocation of transcription factor E3,which further activated AMP-activated protein kinase-S-phase kinase-associated protein 2-coactivator-associated arginine methyltransferase 1 and AMP-activated protein kinase-mammalian target of rapamycin signaling pathways.Intravenous injection of transcription factor E3 sh RNA or intraperitoneal injection of compound C,an AMP-activated protein kinase blocker,inhibited the effects of bexarotene.These findings suggest that bexarotene regulates nuclear translocation of transcription factor E3 through the AMP-activated protein kinase-Sphase kinase-associated protein 2-coactivator-associated arginine methyltransferase 1 and AMP-activated protein kinase-mammalian target of rapamycin signal pathways,promotes autophagy,decreases reactive oxygen species level,inhibits pyroptosis,and improves motor function after spinal cord injury.
基金the Natural Science Innovation Group Foundation of China under Grants NO.41721003the Science and Technology Support Project of Department of Natural Resources of Hubei Province under Grants NO.ZRZY2022KJ29+1 种基金the Special Fund of Hubei Luojia Laboratory under Grants NO.220100020the National Natural Science Foundation of China under Grants NO.42174030.
文摘The study of inter-system bias(ISB)is important for multi-system fusion and the performance of different signal compatibility.In this paper,the stability of ISB at the BDS3/BDS2 receiver end is calculated and analyzed for different time spans(DOY 060~090 in 2021)from a total of 31 MGEX and iGMAS stations.We adopted two estimation strategies,random walk and constant approach,using the precision products of orbit and clock bias provided by WUM,the influence of which on ISB was also analyzed.Our results showed that the ISB value varied little within a day,and the mean of daily ISB standard deviation was only 0.037 m when the observation condition was good.The signal reception was continuous,indicating a high ISB stability for one day.If extending the time series to one month,however,the ISB standard deviation calculated by constant approach,in which a constant ISB is estimated on a daily basis was about 0.1 m,and the results of adjacent days were not continuous,with no apparent pattern.Concerning the random walk approach,the obtained ISB time series also had a jump,and the conclusion was the same as that of the constant strategy.Besides,receiver types showed a strong regularity in ISB numerical situation,and the distribution of ISB values corresponding to the same receiver type was relatively close.Therefore,we conclude that the ISB parameters remain stable in the short term(one day)and less stable in the long-term period.It is recommended that the ISB term should be set as a constant estimate every day in BDS3/BDS2 solutions,regardless of receiver type consistency.
基金supported by the National Natural Science Foundation of China (Grant Nos: 42088101 & 42175033)the High-Performance Computing Center of Nanjing University of Information Science & Technology
文摘Northeast China(NEC)is China’s national grain production base,and the local precipitation is vital for agriculture during the springtime.Therefore,understanding the dynamic origins of the NEC spring rainfall(NECSR)variability is of socioeconomic importance.This study reveals an interdecadal change in the atmospheric teleconnections associated with the NECSR during a recent 60-year period(1961-2020).Before the mid-1980s,NECSR had been related to a Rossby wave train that is coupled with extratropical North Atlantic sea surface temperature(SST),whereas,since the mid-1980s,NECSR has been linked to a quite different Rossby wave train that is coupled with tropical North Atlantic SST.Both Rossby wave trains could lead to enhanced NECSR through anomalous cyclones over East Asia.The weakening of the westerly jet over North America is found to be mainly responsible for the alternation of the atmospheric teleconnections associated with NECSR during two epochs.
基金National Natural Science Foundation of China,Grant/Award Number:3147205731802021.Specialized Research Fund for Laboratory Animal Science of PLA,Grant/Award Number:SYDW[2020]01SYDW[2020]02.
文摘Background:Hundreds of single-nucleotide polymorphism(SNP)sites have been found to be potential genetic markers of type 2 diabetes mellitus(T2DM).However,SNPs related to T2DM in minipigs have been less reported.This study aimed to screen the T2DM-susceptible candidate SNP loci in Bama minipigs so as to improve the success rate of the minipig T2DM model.Methods:The genomic DNAs of three Bama minipigs with T2DM,six sibling lowsusceptibility minipigs with T2DM,and three normal control minipigs were compared by whole-genome sequencing.The T2DM Bama minipig-specific loci were obtained,and their functions were annotated.Meanwhile,the Biomart software was used to perform homology alignment with T2DM-related loci obtained from the human genome-wide association study to screen candidate SNP markers for T2DM in Bama miniature pigs.Results:Whole-genome resequencing detected 6960 specific loci in the minipigs with T2DM,and 13 loci corresponding to 9 diabetes-related genes were selected.Further,a set of 122 specific loci in 69 orthologous genes of human T2DM candidate genes were obtained in the pigs.Collectively,a batch of T2DM-susceptible candidate SNP markers in Bama minipigs,covering 16 genes and 135 loci,was established.Conclusions:Whole-genome sequencing and comparative genomics analysis of the orthologous genes in pigs that corresponded to the human T2DM-related variant loci successfully screened out T2DM-susceptible candidate markers in Bama miniature pigs.Using these loci to predict the susceptibility of the pigs before constructing an animal model of T2DM may help to establish an ideal animal model.
基金the National Natural Science Foundation of China(Grant No.11704093)the National Natural Science Foundation of China(Grant No.12174345)+2 种基金the Opening Project of Shanghai Key Laboratory of Special Artificial Microstructure Materials and Technologythe Jiangsu Key Disciplines of the Fourteenth Five-Year Plan(Grant No.2021135)Zhejiang Provincial Natural Science Foundation of China(Grant No.LZ22A040002)。
文摘Quantum heat transport is considered as an indispensable branch of quantum thermodynamics to potentially improve performance of thermodynamic devices.We theoretically propose a dissipative qubit-photon system composed of multiple coupled resonators interacting with a single two-level qubit,to explore the steady-state heat transport by tuning both the inter-resonator photon hopping and the qubit-photon coupling.Specifically in the three-mode case,the dramatic enhancement and suppression of the heat current into the central resonator can be modulated by the corresponding frequency,compared to the currents into two edge resonators.Moreover,fruitful cycle current components are unraveled at weak qubit-photon coupling,which are crucial to exhibit the nonmonotonic feature with increase of the reservoir temperature bias.In the one-dimensional case under the mean-field framework,the influence of the photon hopping on heat transport is analyzed.The steady-state heat current is comparatively enhanced to the single-mode limit at weak qubit-photon coupling,stemming from the nonvanishing mean-field photon excitation parameter and the additional cycle current component.We hope these obtained results may have possible applications in quantum thermodynamic manipulation and energy harvesting.
基金supported in part by the Major Scientific and Technological Projects of CNPC under Grant ZD2019-183-006.
文摘Link prediction,also known as Knowledge Graph Completion(KGC),is the common task in Knowledge Graphs(KGs)to predict missing connections between entities.Most existing methods focus on designing shallow,scalable models,which have less expressive than deep,multi-layer models.Furthermore,most operations like addition,matrix multiplications or factorization are handcrafted based on a few known relation patterns in several wellknown datasets,such as FB15k,WN18,etc.However,due to the diversity and complex nature of real-world data distribution,it is inherently difficult to preset all latent patterns.To address this issue,we proposeKGE-ANS,a novel knowledge graph embedding framework for general link prediction tasks using automatic network search.KGEANS can learn a deep,multi-layer effective architecture to adapt to different datasets through neural architecture search.In addition,the general search spacewe designed is tailored forKGtasks.We performextensive experiments on benchmark datasets and the dataset constructed in this paper.The results show that our KGE-ANS outperforms several state-of-the-art methods,especially on these datasets with complex relation patterns.
