Objective:Age-relate cataract(ARC)is a disease of the eyes with no effective drugs to prevent or treat patients.The aim of the present study is to determine whether histone H3,αA-crystallin(CRYAA),β-galactosidase(GL...Objective:Age-relate cataract(ARC)is a disease of the eyes with no effective drugs to prevent or treat patients.The aim of the present study is to determine whether histone H3,αA-crystallin(CRYAA),β-galactosidase(GLB1),and p53 are involved in the pathogenesis of ARC.Methods:A total of 99 anterior lens capsules(ALCs)of patients with ARC of various nuclear grades,ultraviolet models of ALCs,and two human lens epithelial cell lines(FHL-124 and SRA01/04)were used,and the expression of histone H3,CRYAA,GLB1,and p53 were detected by immunoblotting and reverse transcription and real time-quantitative polymerase chain reaction.The association between CRYAA with histone H3,GLB1,and p53 was assessed in FHL-124 and SRA01/04 cells following CRYAA overexpression.Results:Histone H3 and p53 in ALCs of patients with ARC were up-regulated in a grade-dependent manner,and the expression of CRYAA showed a positive association with histone H3,p53,and GLB1.In UV models of ALCs and human lens epithelial cell lines,the expression levels of histone H3,cell apoptosis factors(Bax/Bcl-2,cleaved caspase-3),and inflammation factors(interleukin-6,tumor necrosis factor-α)were all up-regulated.Furthermore,transfection of CRYAA in FHL-124 cells induced overexpression of histone H3.Conclusion:CRYAA-mediated upregulation of histone H3 may be involved in the pathogenesis of ARC.p53 may also have a role in ARC development,but not via the CRYAA-histone H3 axis.The results of the present study may assist in improving our understanding of the pathogenesis of ARC and in identifying potential targets for treatment.展开更多
青藏高原是全球造山带研究的热点地区,此前在青藏高原开展的三维层析成像研究大多基于线性反演方法.本文利用青藏高原东缘及邻区布设的127个宽频带固定地震台站记录的连续波形资料,首先通过噪声互相关提取了3~50 s Rayleigh波群速度频...青藏高原是全球造山带研究的热点地区,此前在青藏高原开展的三维层析成像研究大多基于线性反演方法.本文利用青藏高原东缘及邻区布设的127个宽频带固定地震台站记录的连续波形资料,首先通过噪声互相关提取了3~50 s Rayleigh波群速度频散曲线并反演得到群速度分布,再进一步采用模拟退火法反演了研究区的三维S波速度及泊松比结构.结果显示:(1)松潘—甘孜地块的中下地壳低速异常主要分布在龙日坝断裂带、鲜水河断裂带、龙门山断裂带和岷山隆起所围限的区域,而该区域的中下地壳仅具有中等泊松比值,推测松潘—甘孜地块中下地壳的低速物质可能是青藏高原与扬子块体长期相互作用产生的塑性低速滑脱层;上地壳脆性物质在板块作用下沿中地壳低速滑脱层顶界面发生逆冲增厚,造成龙门山的持续抬升和地形起伏,并在构造边界带形成了应变积累和应力集中;而龙门山断裂带的上地壳低速软弱物质为地壳发生破裂提供了有利条件,从而在某种程度上促进了汶川地震和芦山地震的发生.(2)岷山隆起一带中下地壳的高泊松比异常呈“凸起”形态,结合前人研究发现的较高热流和岩石快速抬升现象,推测岷山隆起一带可能存在岩石圈的拆沉,导致地幔热物质上涌而形成下地壳高泊松比物质.(3)川滇地块的北部和南部具有不同的S波速度和泊松比分布特征.30 km深度下川滇地块北部具有明显的低速异常,而该深度下并不具有明显的高泊松比值特征;此外剖面成像结果也显示川滇地块内的低速异常与高泊松比的分布不一致,因此川滇地块的研究结果不支持下地壳流模型.综合其他地震学证据,本文认为川滇地块的变形模式为上地壳纯剪切增厚,块体变形主要受块体内部的走滑断裂及活动边界断裂控制.展开更多
基金This work was supported by the Nature Science Foundation of China(81470618)the Scientific Research Foundation of First Affiliated Hospital of Harbin Medical University(2017B013).
文摘Objective:Age-relate cataract(ARC)is a disease of the eyes with no effective drugs to prevent or treat patients.The aim of the present study is to determine whether histone H3,αA-crystallin(CRYAA),β-galactosidase(GLB1),and p53 are involved in the pathogenesis of ARC.Methods:A total of 99 anterior lens capsules(ALCs)of patients with ARC of various nuclear grades,ultraviolet models of ALCs,and two human lens epithelial cell lines(FHL-124 and SRA01/04)were used,and the expression of histone H3,CRYAA,GLB1,and p53 were detected by immunoblotting and reverse transcription and real time-quantitative polymerase chain reaction.The association between CRYAA with histone H3,GLB1,and p53 was assessed in FHL-124 and SRA01/04 cells following CRYAA overexpression.Results:Histone H3 and p53 in ALCs of patients with ARC were up-regulated in a grade-dependent manner,and the expression of CRYAA showed a positive association with histone H3,p53,and GLB1.In UV models of ALCs and human lens epithelial cell lines,the expression levels of histone H3,cell apoptosis factors(Bax/Bcl-2,cleaved caspase-3),and inflammation factors(interleukin-6,tumor necrosis factor-α)were all up-regulated.Furthermore,transfection of CRYAA in FHL-124 cells induced overexpression of histone H3.Conclusion:CRYAA-mediated upregulation of histone H3 may be involved in the pathogenesis of ARC.p53 may also have a role in ARC development,but not via the CRYAA-histone H3 axis.The results of the present study may assist in improving our understanding of the pathogenesis of ARC and in identifying potential targets for treatment.
文摘青藏高原是全球造山带研究的热点地区,此前在青藏高原开展的三维层析成像研究大多基于线性反演方法.本文利用青藏高原东缘及邻区布设的127个宽频带固定地震台站记录的连续波形资料,首先通过噪声互相关提取了3~50 s Rayleigh波群速度频散曲线并反演得到群速度分布,再进一步采用模拟退火法反演了研究区的三维S波速度及泊松比结构.结果显示:(1)松潘—甘孜地块的中下地壳低速异常主要分布在龙日坝断裂带、鲜水河断裂带、龙门山断裂带和岷山隆起所围限的区域,而该区域的中下地壳仅具有中等泊松比值,推测松潘—甘孜地块中下地壳的低速物质可能是青藏高原与扬子块体长期相互作用产生的塑性低速滑脱层;上地壳脆性物质在板块作用下沿中地壳低速滑脱层顶界面发生逆冲增厚,造成龙门山的持续抬升和地形起伏,并在构造边界带形成了应变积累和应力集中;而龙门山断裂带的上地壳低速软弱物质为地壳发生破裂提供了有利条件,从而在某种程度上促进了汶川地震和芦山地震的发生.(2)岷山隆起一带中下地壳的高泊松比异常呈“凸起”形态,结合前人研究发现的较高热流和岩石快速抬升现象,推测岷山隆起一带可能存在岩石圈的拆沉,导致地幔热物质上涌而形成下地壳高泊松比物质.(3)川滇地块的北部和南部具有不同的S波速度和泊松比分布特征.30 km深度下川滇地块北部具有明显的低速异常,而该深度下并不具有明显的高泊松比值特征;此外剖面成像结果也显示川滇地块内的低速异常与高泊松比的分布不一致,因此川滇地块的研究结果不支持下地壳流模型.综合其他地震学证据,本文认为川滇地块的变形模式为上地壳纯剪切增厚,块体变形主要受块体内部的走滑断裂及活动边界断裂控制.
