柳杉毛虫是一种严重为害柳杉的食叶害虫,探明柳杉毛虫的寄生蝇种类,将为该虫优势天敌的筛选提供更多的选择。本研究从野外采集柳杉毛虫幼虫并带回室内饲养,出蛆化蛹,羽化获得不同种类的寄生蝇。对获得的寄生蝇进行分类、鉴定,并描述其...柳杉毛虫是一种严重为害柳杉的食叶害虫,探明柳杉毛虫的寄生蝇种类,将为该虫优势天敌的筛选提供更多的选择。本研究从野外采集柳杉毛虫幼虫并带回室内饲养,出蛆化蛹,羽化获得不同种类的寄生蝇。对获得的寄生蝇进行分类、鉴定,并描述其系统分类地位、形态特征、地理分布及寄主。调查发现,柳杉毛虫的寄生蝇由多到少依次为蚕饰腹寄蝇Blepharipa zebina(Walker),瘦钩鬃麻蝇Sarcophaga(Sarcorohdendorfia)gracilior(Chen),黑角狭颊寄蝇Carcelia(s.str.)nigrantennata Chao et Liang,松毛虫狭颊寄蝇C.(s.str.)matsukarehae(Shima),星毛虫狭颊寄蝇C.(s.str.)illiberisi Chao et Liang,拟庸赘寄蝇Drino(Palexorista)inconspicuoides(Baranov),羚足鬃麻蝇S.(Sarcorohdendorfia)antilope Böttcher,共7种,其中蚕饰腹寄蝇、瘦钩鬃麻蝇、黑角狭颊寄蝇分布较广,种群数量较多,寄生率较高,分别为7.44%、7.37%、5.48%,被认为是优势种;且拟庸赘寄蝇为福建新分布记录;柳杉毛虫是黑角狭颊寄蝇、星毛虫狭颊寄蝇、拟庸赘寄蝇的新寄主;还发现2种重寄生蜂寄生于寄生蝇。记述柳杉毛虫的7种寄生蝇形态特征,有助于今后识别其天敌昆虫,研究害虫与天敌的种间关系,评价寄生蝇种群对维护柳杉林分生态系统稳定和防控柳杉毛虫的潜能,具有重要意义。展开更多
Resistance mechanisms of rho-associated kinase（ROCK） inhibitors are associated with the enhanced expression of cyclooxygenase-2（COX-2）. The therapeutic effects of ROCK on nervous system diseases might be enhanced ...Resistance mechanisms of rho-associated kinase（ROCK） inhibitors are associated with the enhanced expression of cyclooxygenase-2（COX-2）. The therapeutic effects of ROCK on nervous system diseases might be enhanced by COX-2 inhibitors. This study investigated the synergistic effect of the combined use of the ROCK inhibitor fasudil and a COX-2 inhibitor celecoxib on spinal cord injury in a rat model established by transecting the right half of the spinal cord at T11. Rat models were orally administrated with celecoxib（20 mg/kg） and/or intramuscularly with fasudil（10 mg/kg） for 2 weeks. Results demonstrated that the combined use of celecoxib and fasudil significantly decreased COX-2 and Rho kinase II expression surrounding the lesion site in rats with spinal cord injury, improved the pathomorphology of the injured spinal cord, and promoted the recovery of motor function. Moreover, the effects of the drug combination were better than celecoxib or fasudil alone. This study demonstrated that the combined use of fasudil and celecoxib synergistically enhanced the functional recovery of injured spinal cord in rats.展开更多
Objective： This review aimed to examine the effectiveness of clinical nurse specialist （CNS） interventions in patients with chronic obstructive pulmonary disease （COPD）. COPD significantly affects p...Objective： This review aimed to examine the effectiveness of clinical nurse specialist （CNS） interventions in patients with chronic obstructive pulmonary disease （COPD）. COPD significantly affects people＇s health worldwide. With the development in nursing, CNSs are playing increasingly important roles in different departments. However, the studies on the effectiveness of CNSs in COPD are not as well organized as the studies on the effectiveness of CNSs in bronchiectasis and asthma. Therefore, this review aims to find some updated evidence on the CNS interventions for patients with COPD and on whether these interventions are effective.Methods： A narrative analysis of the data was performed for the eligible studies. Four databases were chosen： CINAHL, MEDLINE, British Nursing Index, and Cochrane Library. Other websites such as the National Institute for Health and Clinical Excellence, National Health Service Evidence, Association of Respiratory Nurse Specialists, and National Association of Clinical Nurse Specialist were searched as well. Two reviewers performed study identification independently, and all the retrieved articles were stored using the EndNote X7 software. The risk of bias in the included studies was assessed using the Cochrane Collaboration＇s risk of bias tool. Results： A total of nine studies were included in this review. There were five current interventions by CNSs for patients with COPD. These interventions were home nursing support, CNS]s supported discharge, multidisciplinary cooperation programs, nurse-led care programs, and self-care management education. The effectiveness of these five interventions was evaluated individually. There is low- to moderate-quality evidence indicating that home nursing support interventions may have a positive effect on mortality and quality of life. No significant difference in quality of life has been found between the CNS-supported discharge intervention and the usual service. The multidisciplinary cooperation program probably had a positive effect on quality ot life in patients with COPD. Both nurse-led care and self-care management education intervention had a positive effect on mortality of patients with COPD.Conclusions： The findings of this review provide updated evidence on the effectiveness of CNS interventions for patients with COPD. Although nine trials were included and five types of interventions were identified, there is still lack of high-quality evidence.展开更多
In this study, PC12 Adh cells and Neuro-2a cells were treated with Rho-associated kinase inhibitors （Y27632 and Fasudil）, a cyclooxygenase-1 selective inhibitor （SC560）, and a cyclooxygenase-2 inhibitor （NS398）....In this study, PC12 Adh cells and Neuro-2a cells were treated with Rho-associated kinase inhibitors （Y27632 and Fasudil）, a cyclooxygenase-1 selective inhibitor （SC560）, and a cyclooxygenase-2 inhibitor （NS398）. We found that these cells became tolerant to Rho-associated kinase inhibitors, as neurite outgrowth induced by these inhibitors diminished following more than 3 days of exposure in either cell line. The proteins cyclooxygenase-2 and cytosolic prostaglandin E synthetase were upregulated at day 3. NS398 decreased the tolerance to neurite outgrowth induction in both cell lines, whereas SC560 had almost no effect. These findings indicate that cells become tolerant to neurite outgrowth induced by Rho-associated kinase inhibitors, this is at least partly associated with upregulation of proteins involved in the cyclooxygenase-2 pathway, and cyclooxygenases-2 inhibition prevents this tolerance.展开更多
Objective: In this study, we evaluated the effect of preemptive analgesia of flurbiprofen axetil (FA) and tramadol on immune response in patients undergoing radical mastectomy. Methods: In this prospective randomized,...Objective: In this study, we evaluated the effect of preemptive analgesia of flurbiprofen axetil (FA) and tramadol on immune response in patients undergoing radical mastectomy. Methods: In this prospective randomized, double-blind, placebo-controlled study, 80 patients were randomly assigned to one of four groups (n = 20). The FA group (group A) patients received FA before tracheal intubation and at the end of surgery. The tramadol group (group B) patients received tramadol before tracheal intubation and at the end of surgery. The control group (group C) patients received saline before tracheal intubation and at the end of surgery. The combination group (group D) patients received FA and tramadol before tracheal intubation and at the end of surgery. Cluster of differentiation and lymphocytes were measured. Results: The CD3, CD4, CD8 and lymphocytes decreased postoperatively of all groups except of group A and D at 0.5 h postoperatively (P < 0.05). The CD3, CD4, CD8 and lymphocytes of group D were higher than those of group C at 0.5 h and 4 h postoperatively (P < 0.05). Conclusion: Preemptive analgesia using flurbiprofen axetil (FA) and tramadol may protect the immune system. The immune protective effect of FA may be better than that of tramadol. The combination of FA and tramadol may be the most effective among all the strategies.展开更多
PR/SET domain 1(PRDM1)gene is located on chromosome 6q21,encoding the B lymphocyte-induced maturation protein 1(BLIMP1).1 It is reported that loss of PRDM1 function is exacerbated in activated B-cell-like(ABC)-diffuse...PR/SET domain 1(PRDM1)gene is located on chromosome 6q21,encoding the B lymphocyte-induced maturation protein 1(BLIMP1).1 It is reported that loss of PRDM1 function is exacerbated in activated B-cell-like(ABC)-diffuse large B cell lymphoma(DLBCL)and associated with inferior survival.However,it remains unclear what leads to PRDM1 inactivation and the drug resistance mechanism caused by abnormal inactivation of PRDM1.We investigated the contribution of PRDM1 gene as a prognosis and potential therapeutic target for ABC-DLBCL patients and further clarified the possible mechanism of PRDM1 abnormal inactivation.We first proposed that TP53 could regulate PRDM1 by histone ubiquitination modification at the post-transcriptional level.展开更多
The combined pollution of heavy metals is ubiquitous worldwide.Mn/Al-layered double oxide-loaded crab shells biochar (LDO/BC) was prepared,so as to remediate the combined pollution of Cd and Cu in soil and water.The p...The combined pollution of heavy metals is ubiquitous worldwide.Mn/Al-layered double oxide-loaded crab shells biochar (LDO/BC) was prepared,so as to remediate the combined pollution of Cd and Cu in soil and water.The pristine and used LDO/BC were characterized and the results revealed that the layered double oxide was successfully loaded on crab shells biochar (BC) and metal element Ca in crab shells was beneficial to the formation of more regular layered and flake structure.The maximal adsorption capacity (Qm) of LDO/BC for aqueous Cu^(2+)and Cd^(2+)was 66.23 and 73.47 mg/g,respectively.LDO/BC and BC were used to remediate e-waste-contaminated soil for the first time and exhibited highly efficient performance.The extraction amount of Cu and Cd in the contaminated soil by diethylene triamine penta-acetic acid (DTPA) after treating with 5% LDO/BC was significantly reduced from 819.84 to 205.95 mg/kg (with passivation rate 74.8%) and 8.46 to 4.16 mg/kg(with passivation rate 50.8%),respectively,inferring that the bioavailability of heavy metals declined remarkably.The experimental result also suggested that after remediation by LDO/BC the exchangeable and weak acid soluble Cu and Cd in soil translated to reducible,residual and oxidizable fraction which are more stable state.Precipitation,complexation and ion exchange were proposed as the possible mechanisms for Cd and Cu removal.In general,these experiment results indicate that LDO/BC can be a potentially effective reagent for remediation of heavy metal contaminated water and soil.展开更多
To the Editor:Non-Hodgkin lymphoma(NHL)is a common type of hematological malignancy.Although the development of targeted therapies has improved the survival of patients with aggressive NHL,autologous hematopoietic ste...To the Editor:Non-Hodgkin lymphoma(NHL)is a common type of hematological malignancy.Although the development of targeted therapies has improved the survival of patients with aggressive NHL,autologous hematopoietic stem cell transplantation(HSCT)remains indispensable.There is no standard conditioning regimen for autologous stem cell transplantation(ASCT).展开更多
文摘柳杉毛虫是一种严重为害柳杉的食叶害虫,探明柳杉毛虫的寄生蝇种类,将为该虫优势天敌的筛选提供更多的选择。本研究从野外采集柳杉毛虫幼虫并带回室内饲养,出蛆化蛹,羽化获得不同种类的寄生蝇。对获得的寄生蝇进行分类、鉴定,并描述其系统分类地位、形态特征、地理分布及寄主。调查发现,柳杉毛虫的寄生蝇由多到少依次为蚕饰腹寄蝇Blepharipa zebina(Walker),瘦钩鬃麻蝇Sarcophaga(Sarcorohdendorfia)gracilior(Chen),黑角狭颊寄蝇Carcelia(s.str.)nigrantennata Chao et Liang,松毛虫狭颊寄蝇C.(s.str.)matsukarehae(Shima),星毛虫狭颊寄蝇C.(s.str.)illiberisi Chao et Liang,拟庸赘寄蝇Drino(Palexorista)inconspicuoides(Baranov),羚足鬃麻蝇S.(Sarcorohdendorfia)antilope Böttcher,共7种,其中蚕饰腹寄蝇、瘦钩鬃麻蝇、黑角狭颊寄蝇分布较广,种群数量较多,寄生率较高,分别为7.44%、7.37%、5.48%,被认为是优势种;且拟庸赘寄蝇为福建新分布记录;柳杉毛虫是黑角狭颊寄蝇、星毛虫狭颊寄蝇、拟庸赘寄蝇的新寄主;还发现2种重寄生蜂寄生于寄生蝇。记述柳杉毛虫的7种寄生蝇形态特征,有助于今后识别其天敌昆虫,研究害虫与天敌的种间关系,评价寄生蝇种群对维护柳杉林分生态系统稳定和防控柳杉毛虫的潜能,具有重要意义。
基金supported by the National Natural Science Foundation of ChinaNo.81060109+1 种基金a grant from the Yunnan Provincial Department of Science&Technology in ChinaNo.2008CD150
文摘Resistance mechanisms of rho-associated kinase（ROCK） inhibitors are associated with the enhanced expression of cyclooxygenase-2（COX-2）. The therapeutic effects of ROCK on nervous system diseases might be enhanced by COX-2 inhibitors. This study investigated the synergistic effect of the combined use of the ROCK inhibitor fasudil and a COX-2 inhibitor celecoxib on spinal cord injury in a rat model established by transecting the right half of the spinal cord at T11. Rat models were orally administrated with celecoxib（20 mg/kg） and/or intramuscularly with fasudil（10 mg/kg） for 2 weeks. Results demonstrated that the combined use of celecoxib and fasudil significantly decreased COX-2 and Rho kinase II expression surrounding the lesion site in rats with spinal cord injury, improved the pathomorphology of the injured spinal cord, and promoted the recovery of motor function. Moreover, the effects of the drug combination were better than celecoxib or fasudil alone. This study demonstrated that the combined use of fasudil and celecoxib synergistically enhanced the functional recovery of injured spinal cord in rats.
文摘Objective： This review aimed to examine the effectiveness of clinical nurse specialist （CNS） interventions in patients with chronic obstructive pulmonary disease （COPD）. COPD significantly affects people＇s health worldwide. With the development in nursing, CNSs are playing increasingly important roles in different departments. However, the studies on the effectiveness of CNSs in COPD are not as well organized as the studies on the effectiveness of CNSs in bronchiectasis and asthma. Therefore, this review aims to find some updated evidence on the CNS interventions for patients with COPD and on whether these interventions are effective.Methods： A narrative analysis of the data was performed for the eligible studies. Four databases were chosen： CINAHL, MEDLINE, British Nursing Index, and Cochrane Library. Other websites such as the National Institute for Health and Clinical Excellence, National Health Service Evidence, Association of Respiratory Nurse Specialists, and National Association of Clinical Nurse Specialist were searched as well. Two reviewers performed study identification independently, and all the retrieved articles were stored using the EndNote X7 software. The risk of bias in the included studies was assessed using the Cochrane Collaboration＇s risk of bias tool. Results： A total of nine studies were included in this review. There were five current interventions by CNSs for patients with COPD. These interventions were home nursing support, CNS]s supported discharge, multidisciplinary cooperation programs, nurse-led care programs, and self-care management education. The effectiveness of these five interventions was evaluated individually. There is low- to moderate-quality evidence indicating that home nursing support interventions may have a positive effect on mortality and quality of life. No significant difference in quality of life has been found between the CNS-supported discharge intervention and the usual service. The multidisciplinary cooperation program probably had a positive effect on quality ot life in patients with COPD. Both nurse-led care and self-care management education intervention had a positive effect on mortality of patients with COPD.Conclusions： The findings of this review provide updated evidence on the effectiveness of CNS interventions for patients with COPD. Although nine trials were included and five types of interventions were identified, there is still lack of high-quality evidence.
基金supported by Yunnan Provincial Science and Technology Department, No.2009CD079the National Natural Science Foundation ofChina, No.81060109.
文摘In this study, PC12 Adh cells and Neuro-2a cells were treated with Rho-associated kinase inhibitors （Y27632 and Fasudil）, a cyclooxygenase-1 selective inhibitor （SC560）, and a cyclooxygenase-2 inhibitor （NS398）. We found that these cells became tolerant to Rho-associated kinase inhibitors, as neurite outgrowth induced by these inhibitors diminished following more than 3 days of exposure in either cell line. The proteins cyclooxygenase-2 and cytosolic prostaglandin E synthetase were upregulated at day 3. NS398 decreased the tolerance to neurite outgrowth induction in both cell lines, whereas SC560 had almost no effect. These findings indicate that cells become tolerant to neurite outgrowth induced by Rho-associated kinase inhibitors, this is at least partly associated with upregulation of proteins involved in the cyclooxygenase-2 pathway, and cyclooxygenases-2 inhibition prevents this tolerance.
文摘Objective: In this study, we evaluated the effect of preemptive analgesia of flurbiprofen axetil (FA) and tramadol on immune response in patients undergoing radical mastectomy. Methods: In this prospective randomized, double-blind, placebo-controlled study, 80 patients were randomly assigned to one of four groups (n = 20). The FA group (group A) patients received FA before tracheal intubation and at the end of surgery. The tramadol group (group B) patients received tramadol before tracheal intubation and at the end of surgery. The control group (group C) patients received saline before tracheal intubation and at the end of surgery. The combination group (group D) patients received FA and tramadol before tracheal intubation and at the end of surgery. Cluster of differentiation and lymphocytes were measured. Results: The CD3, CD4, CD8 and lymphocytes decreased postoperatively of all groups except of group A and D at 0.5 h postoperatively (P < 0.05). The CD3, CD4, CD8 and lymphocytes of group D were higher than those of group C at 0.5 h and 4 h postoperatively (P < 0.05). Conclusion: Preemptive analgesia using flurbiprofen axetil (FA) and tramadol may protect the immune system. The immune protective effect of FA may be better than that of tramadol. The combination of FA and tramadol may be the most effective among all the strategies.
基金supported by the National Natural Science Foundation of China(No.81720108002,81700193,82170186)Jiangsu Province's Medical Elite Programme(China)(No.ZDRCA2016022)+4 种基金Project of National Key Clinical Specialty(China),Jiangsu Provincial Special Program of Medical Science(China)(No.BE2017751)National Science and Technology Major F Project(China)(No.2018ZX09734007)Nature Science Foundation for Youths of Jiangsu Province,China(No.BK20220719)China Postdoctoral Science Foundation(No.2021M691336)Jiangsu Post doctoral Science Foundation(China)(No.2021K083A).
文摘PR/SET domain 1(PRDM1)gene is located on chromosome 6q21,encoding the B lymphocyte-induced maturation protein 1(BLIMP1).1 It is reported that loss of PRDM1 function is exacerbated in activated B-cell-like(ABC)-diffuse large B cell lymphoma(DLBCL)and associated with inferior survival.However,it remains unclear what leads to PRDM1 inactivation and the drug resistance mechanism caused by abnormal inactivation of PRDM1.We investigated the contribution of PRDM1 gene as a prognosis and potential therapeutic target for ABC-DLBCL patients and further clarified the possible mechanism of PRDM1 abnormal inactivation.We first proposed that TP53 could regulate PRDM1 by histone ubiquitination modification at the post-transcriptional level.
基金supported by the National Key Research and Development Program of China (No. 2018YFC1802800)the Local Innovation and Entrepreneurship Team Project of Guangdong Special Support Program (No. 2019BT02L218)+1 种基金the National Natural Science Foundation of China (Nos. 41673091, U1501234)the Guangdong Science and Technology Program (No. 2020B121201003)。
文摘The combined pollution of heavy metals is ubiquitous worldwide.Mn/Al-layered double oxide-loaded crab shells biochar (LDO/BC) was prepared,so as to remediate the combined pollution of Cd and Cu in soil and water.The pristine and used LDO/BC were characterized and the results revealed that the layered double oxide was successfully loaded on crab shells biochar (BC) and metal element Ca in crab shells was beneficial to the formation of more regular layered and flake structure.The maximal adsorption capacity (Qm) of LDO/BC for aqueous Cu^(2+)and Cd^(2+)was 66.23 and 73.47 mg/g,respectively.LDO/BC and BC were used to remediate e-waste-contaminated soil for the first time and exhibited highly efficient performance.The extraction amount of Cu and Cd in the contaminated soil by diethylene triamine penta-acetic acid (DTPA) after treating with 5% LDO/BC was significantly reduced from 819.84 to 205.95 mg/kg (with passivation rate 74.8%) and 8.46 to 4.16 mg/kg(with passivation rate 50.8%),respectively,inferring that the bioavailability of heavy metals declined remarkably.The experimental result also suggested that after remediation by LDO/BC the exchangeable and weak acid soluble Cu and Cd in soil translated to reducible,residual and oxidizable fraction which are more stable state.Precipitation,complexation and ion exchange were proposed as the possible mechanisms for Cd and Cu removal.In general,these experiment results indicate that LDO/BC can be a potentially effective reagent for remediation of heavy metal contaminated water and soil.
基金National Natural Science Foundation of China(Nos.81770166,81700193,82170186 and 81720108002)Jiangsu Province’s Medical Elite Programme(No.ZDRCA2016022)+4 种基金Project of National Key Clinical Specialty,Jiangsu Provincial Special Program of Medical Science(No.BE2017751)National Science and Technology Major Project(No.2018 ZX09734007)China Postdoctoral Science Foundation(No.2021M691336)Jiangsu Postdoctoral Science Foundation(No.2021K083A)Translational Research Grant of NCRCH(No.2020ZKZB01)
文摘To the Editor:Non-Hodgkin lymphoma(NHL)is a common type of hematological malignancy.Although the development of targeted therapies has improved the survival of patients with aggressive NHL,autologous hematopoietic stem cell transplantation(HSCT)remains indispensable.There is no standard conditioning regimen for autologous stem cell transplantation(ASCT).
