The seaweed Ulva prolifera exists in 2 different states; attached to rocks or free-floating. However, there is little difference between the structures of the 2 states. U. prolifera thalli show significant differences...The seaweed Ulva prolifera exists in 2 different states; attached to rocks or free-floating. However, there is little difference between the structures of the 2 states. U. prolifera thalli show significant differences in growth rate, with the attached thalli growing at a normal rate and free-floating thalli growing at a much faster rate. This raised the possibility that the growth of the two states may be regulated differently. miRNAs are important post-transcriptional regulators. In higher plants and animals, miRNAs have been extensively studied but they have been rarely studied in algae. To identify U. prolifera miRNAs and to investigate their possible roles in proliferation, we constructed and sequenced small RNA (sRNA) libraries from U. prolifera. Our results show that U. prolifera has a complex small RNA system that might play important roles in various processes.展开更多
Pseudogenes share sequence similarities with functional genes,but in general they have lost their protein-coding ability.The identification of pseudogenes is a very important step in genome annotation.Phaeodactylum tr...Pseudogenes share sequence similarities with functional genes,but in general they have lost their protein-coding ability.The identification of pseudogenes is a very important step in genome annotation.Phaeodactylum tricornutum is a marine diatom that is rich in polyunsaturated fatty acids(PUFAs).The genome of P.tricornutum has been completely sequenced.To identify pseudogenes in P.tricornutum,we developed a pipeline to discover and characterize pseudogenes.We identified a total of 1654 'true' processed pseudogenes,714 duplicated pseudogenes and 4729 pseudogene fragments.The results of the bioinformatics analysis indicated that the genome sequence of P.tricornutum contained many pseudogenes and pseudogene fragments.展开更多
基金supported by the Scientific and Technical Supporting Programs of China (2008BAC49B01)the National Natural Science Foundation of China (30830015)
文摘The seaweed Ulva prolifera exists in 2 different states; attached to rocks or free-floating. However, there is little difference between the structures of the 2 states. U. prolifera thalli show significant differences in growth rate, with the attached thalli growing at a normal rate and free-floating thalli growing at a much faster rate. This raised the possibility that the growth of the two states may be regulated differently. miRNAs are important post-transcriptional regulators. In higher plants and animals, miRNAs have been extensively studied but they have been rarely studied in algae. To identify U. prolifera miRNAs and to investigate their possible roles in proliferation, we constructed and sequenced small RNA (sRNA) libraries from U. prolifera. Our results show that U. prolifera has a complex small RNA system that might play important roles in various processes.
基金supported by the National Basic Projects for Science and Technology (SQ2012FY4910019-1)the Nonprofit Research Project for the State Oceanic Administration (201105008-2)the National Natural Science Foundation of China (41176137)
文摘Pseudogenes share sequence similarities with functional genes,but in general they have lost their protein-coding ability.The identification of pseudogenes is a very important step in genome annotation.Phaeodactylum tricornutum is a marine diatom that is rich in polyunsaturated fatty acids(PUFAs).The genome of P.tricornutum has been completely sequenced.To identify pseudogenes in P.tricornutum,we developed a pipeline to discover and characterize pseudogenes.We identified a total of 1654 'true' processed pseudogenes,714 duplicated pseudogenes and 4729 pseudogene fragments.The results of the bioinformatics analysis indicated that the genome sequence of P.tricornutum contained many pseudogenes and pseudogene fragments.
