Flavonoids are widely-distributed polyphenolic secondary metabolites with diverse biological activities in plants and benefit human health as protective dietary agents.They participate in plants' responses to hars...Flavonoids are widely-distributed polyphenolic secondary metabolites with diverse biological activities in plants and benefit human health as protective dietary agents.They participate in plants' responses to harsh environmental conditions and effectively regulate the cell differentiation and growth.In plants,the majority of their functions are attributed to their strong antioxidative properties.Similarly,dietary flavonoids protect the human body against free radicals which are associated with the development of cancer and atherosclerosis.Plants rich in polyphenols have been used to cure various diseases because of their antibacterial,antiviral,antifungal and anticancer properties.This review summarizes the up-to-date research trends and development on flavonoids and its derivatives,working mechanisms and potential functions and applications particularly in relation to plant protection and human health.Towards the end,notable concluding remarks with a close-up look at the future research directions have also been presented briefly.展开更多
Dissolved organic nitrogen (DON) represents a significant pool of soluble nitrogen (N) in soil ecosystems.Soil samples under three different horticultural management practices were collected from the Xiaxiyang Organic...Dissolved organic nitrogen (DON) represents a significant pool of soluble nitrogen (N) in soil ecosystems.Soil samples under three different horticultural management practices were collected from the Xiaxiyang Organic Vegetable and Fruit Farm,Shanghai,China,to investigate the dynamics of N speciation during 2 months of aerobic incubation,to compare the effects of different soils on the mineralization of 14 C-labeled amino acids and peptides,and to determine which of the pathways in the decomposition and subsequent ammonification and nitrification of organic N represented a significant blockage in soil N supply.The dynamics of N speciation was found to be significantly affected by mineralization and immobilization.DON,total free amino acids,and NH + 4-N were maintained at very low levels and did not accumulate,whereas NO 3-N gradually accumulated in these soils.The conversion of insoluble organic N to low-molecular-weight (LMW) DON represented a main constraint to N supply,while conversions of LMW DON to NH + 4-N and NH + 4-N to NO 3-N did not.Free amino acids and peptides were rapidly mineralized in the soils by the microbial community and consequently did not accumulate in soil.Turnover rates of the additional amino acids and peptides were soil-dependent and generally followed the order of organic soil > transitional soil > conventional soil.The turnover of high-molecular-weight DON was very slow and represented the major DON loss.Further studies are needed to investigate the pathways and bottlenecks of organic N degradation.展开更多
In this study, we established a dynamic morphological model using the accumulated thermal effectiveness and photosynthetic active radiation (PAR) (A-TEP), aiming to explore the relationship between muskmelon (Cuc...In this study, we established a dynamic morphological model using the accumulated thermal effectiveness and photosynthetic active radiation (PAR) (A-TEP), aiming to explore the relationship between muskmelon (Cucumis melo L.) fruit attributes and environmental factors. Muskmelon surface color was described by parameters of red, green, blue, hue, saturation and brightness (HSI). Three characteristic parameters, gray level co-occurrence matrix (GLCM), angular second moment (ASM), entropy, contrast, and the coverage rate were used to describe the process of muskmelon fruit netting formation. ASM was not significant difference during muskmelon fruit growth. The number and deep of netting stripes gradually increased with fruit growth. Coverage rate increased rapidly for 15-30 d after pollination. The vertical and horizontal diameters of muskmelon fruit were followed a logistic curve. And root mean squared errors (RMSE) between the simulated and measured vertical and horizontal diameters were 3.527 and 4.696 mm, respectively. RMSE of red, green, blue, saturation and brightness were 0.999, 2.690, 2.992, 0.033 and 5.51, respectively, and the RMSE for entropy, contrast and coverage rates were 0.077, 0.063 and 0.015, respectively, indicating a well consistent between measured and simulated values.展开更多
Different nitrogen (N) forms may cause changes in the metabolic profiles of plants. However, few studies have been conducted on the effects of amino acid-N on plant metabolic profiles. The main objective of this stu...Different nitrogen (N) forms may cause changes in the metabolic profiles of plants. However, few studies have been conducted on the effects of amino acid-N on plant metabolic profiles. The main objective of this study was to identify primary metabolites associated with amino acid-N (Gly, Gln and Ala) through metabolic profile analysis using gas chromatography- mass spectrometry (GC-MS). Plants of pakchoi (Brassica campestris L. ssp. chinensis L.), Huawang and Wuyueman cultivars, were grown with different nitrogen forms (i.e., Gly, Gln, Ala, NO3-N, and N starvation) applied under sterile hydroponic conditions. The fresh weight and plant N accumulation of Huawang were greater than those of Wuyueman, which indicates that the former exhibited better N-use efficiency than the latter. The physiological performances of the applied N forms were generally in the order of NO3-N〉Gln〉Gly〉Ala. The metabolic analysis of leaf polar extracts revealed 30 amino acid N-responsive metabolites in the two pakchoi cultivars, mainly consisting of sugars, amino acids, and organic acids. Changes in the carbon metabolism of pakchoi leaves under amino acid treatments occurred via the accumulation of fructose, glucose, xylose, and arabinose. Disruption of amino acid metabolism resulted in accumulation of endogenous Gly in Gly treatment, Pro in Ala treatment, and Asn in three amino acid (Gly, Gln and Ala) treatments. By contrast, the levels of endogenous Gln and Leu decreased. However, this reduction varied among cultivars and amino acid types. Amino acid-N supply also affected the citric acid cycle, namely, the second stage of respiration, where leaves in Gly, Gln and Ala treatments contained low levels of malic, citric and succinic acids compared with leaves in NO3-N treatments. No significant difference in the metabolic responses was observed between the two cultivars which differed in their capability to use N. The response of primary metabolites in pakchoi leaves to amino acid-N supply may serve an important function in pakchoi adaptation to amino acid-N sources.展开更多
Utilization of organic nitrogen (N) is an important aspect of plant N assimilation and has potential application in sustainable agriculture. The aim of this study was to investigate the plant growth, C and N accumul...Utilization of organic nitrogen (N) is an important aspect of plant N assimilation and has potential application in sustainable agriculture. The aim of this study was to investigate the plant growth, C and N accumulation in leaves and roots of tomato seedlings in response to inorganic (NH4^+-N, NO3^-N) and organic nitrogen (Gly-N). Different forms of nitrogen (NH4^+-N, NO3^--N, Gly-N) were supplied to two tomato cultivars (Shenfen 918 and Huying 932) using a hydroponics system. The plant dry biomass, chlorophyll content, root activity, total carbon and nitrogen content in roots and leaves, and total N absorption, etc. were assayed during the cultivation. Our results showed that no significant differences in plant height, dry biomass, and total N content were found within the first 16 d among three treatments; however, significant differences in treatments on 24 d and 32 d were observed, and the order was NO3^--N 〉 Gly-N 〉 NH4^+-N. Significant differences were also observed between the two tomato cultivars. Chlorophyll contents in the two cultivars were significantly increased by the Gly-N treatment, and root activity showed a significant decrease in NHa^+-N treatment. Tomato leaf total carbon content was slightly affected by different N forms; however, total carbon in root and total nitrogen in root and leaf were promoted significantly by inorganic and organic N. Among the applied N forms, the increasing effects of the NH4^+-N treatment were larger than that of the Gly-N. In a word, different N resources resulted in different physiological effects in tomatoes. Organic nitrogen (e.g., Gly-N) can be a proper resource of plant N nutrition. Tomatoes of different genotypes had different responses under organic nitrogen (e.g., Gly-N) supplies.展开更多
Salinity is one of the most important abiotic stresses that adversely affects crop growth and productivity. A subtractive suppression hybridization (SSH) library were constructed from the roots of salt-sensitive Yul...Salinity is one of the most important abiotic stresses that adversely affects crop growth and productivity. A subtractive suppression hybridization (SSH) library were constructed from the roots of salt-sensitive Yulu cultivar melon seedlings under salt stress; 557 high-quality expressed sequence tags (ESTs) were randomly sequenced, with an average size of 428 bp, which assembled into 68 contigs and 315 singletons. Compared with our previous SSH library generated from the salt-tolerant Bingxuecui cultivar, the proportion of transcripts involved in metabolism, protein fate, cellular communication/signal transduction mechanisms, and cell rescue/defense were 4, 1.46, 0.94, and 0.4% higher, respectively, in the salt-tolerant cultivar than the in salt-sensitive cultivar. Quantitative real-time PCR analysis of eleven transcripts revealed temporal variations in their expression in the two cultivars under salt stress. One NAC gene (JZ477011) was heterologously expressed in yeast for functional characterization, and enhanced the sensitivity of yeast cells to high-salinity to salt stress and inhibited their growth. Information regards to their functions would aid in the understanding of response mechanisms to saline stress and in the development of molecular markers for selecting salt-tolerant melon cultivars.展开更多
基金supported by the National High-Tech R&D Program of China (863 Program,2013AA103000)the earmarked fund for Shanghai Modern Leaf Vegetable Industry Technology Research System,China (201802)
文摘Flavonoids are widely-distributed polyphenolic secondary metabolites with diverse biological activities in plants and benefit human health as protective dietary agents.They participate in plants' responses to harsh environmental conditions and effectively regulate the cell differentiation and growth.In plants,the majority of their functions are attributed to their strong antioxidative properties.Similarly,dietary flavonoids protect the human body against free radicals which are associated with the development of cancer and atherosclerosis.Plants rich in polyphenols have been used to cure various diseases because of their antibacterial,antiviral,antifungal and anticancer properties.This review summarizes the up-to-date research trends and development on flavonoids and its derivatives,working mechanisms and potential functions and applications particularly in relation to plant protection and human health.Towards the end,notable concluding remarks with a close-up look at the future research directions have also been presented briefly.
基金Project supported by the National High Technology Research and Development Program (863 program) of China(No. 2006AA10A311)the National Natural Science Foundation of China (No. 40901124)the Shanghai Leading Aca-demic Discipline Program,China (No. B209)
文摘Dissolved organic nitrogen (DON) represents a significant pool of soluble nitrogen (N) in soil ecosystems.Soil samples under three different horticultural management practices were collected from the Xiaxiyang Organic Vegetable and Fruit Farm,Shanghai,China,to investigate the dynamics of N speciation during 2 months of aerobic incubation,to compare the effects of different soils on the mineralization of 14 C-labeled amino acids and peptides,and to determine which of the pathways in the decomposition and subsequent ammonification and nitrification of organic N represented a significant blockage in soil N supply.The dynamics of N speciation was found to be significantly affected by mineralization and immobilization.DON,total free amino acids,and NH + 4-N were maintained at very low levels and did not accumulate,whereas NO 3-N gradually accumulated in these soils.The conversion of insoluble organic N to low-molecular-weight (LMW) DON represented a main constraint to N supply,while conversions of LMW DON to NH + 4-N and NH + 4-N to NO 3-N did not.Free amino acids and peptides were rapidly mineralized in the soils by the microbial community and consequently did not accumulate in soil.Turnover rates of the additional amino acids and peptides were soil-dependent and generally followed the order of organic soil > transitional soil > conventional soil.The turnover of high-molecular-weight DON was very slow and represented the major DON loss.Further studies are needed to investigate the pathways and bottlenecks of organic N degradation.
基金supported by the National Natural Science Foundation of China (31471411)the Shanghai Agriculture Applied Technology Development Program,China ((2017)3-8-4)
文摘In this study, we established a dynamic morphological model using the accumulated thermal effectiveness and photosynthetic active radiation (PAR) (A-TEP), aiming to explore the relationship between muskmelon (Cucumis melo L.) fruit attributes and environmental factors. Muskmelon surface color was described by parameters of red, green, blue, hue, saturation and brightness (HSI). Three characteristic parameters, gray level co-occurrence matrix (GLCM), angular second moment (ASM), entropy, contrast, and the coverage rate were used to describe the process of muskmelon fruit netting formation. ASM was not significant difference during muskmelon fruit growth. The number and deep of netting stripes gradually increased with fruit growth. Coverage rate increased rapidly for 15-30 d after pollination. The vertical and horizontal diameters of muskmelon fruit were followed a logistic curve. And root mean squared errors (RMSE) between the simulated and measured vertical and horizontal diameters were 3.527 and 4.696 mm, respectively. RMSE of red, green, blue, saturation and brightness were 0.999, 2.690, 2.992, 0.033 and 5.51, respectively, and the RMSE for entropy, contrast and coverage rates were 0.077, 0.063 and 0.015, respectively, indicating a well consistent between measured and simulated values.
基金the National High-Tech R&D Program of China(863,2012AA101903)the Special Fund of China for Agro-Scientific Research in the Public Interest(200903056)
文摘Different nitrogen (N) forms may cause changes in the metabolic profiles of plants. However, few studies have been conducted on the effects of amino acid-N on plant metabolic profiles. The main objective of this study was to identify primary metabolites associated with amino acid-N (Gly, Gln and Ala) through metabolic profile analysis using gas chromatography- mass spectrometry (GC-MS). Plants of pakchoi (Brassica campestris L. ssp. chinensis L.), Huawang and Wuyueman cultivars, were grown with different nitrogen forms (i.e., Gly, Gln, Ala, NO3-N, and N starvation) applied under sterile hydroponic conditions. The fresh weight and plant N accumulation of Huawang were greater than those of Wuyueman, which indicates that the former exhibited better N-use efficiency than the latter. The physiological performances of the applied N forms were generally in the order of NO3-N〉Gln〉Gly〉Ala. The metabolic analysis of leaf polar extracts revealed 30 amino acid N-responsive metabolites in the two pakchoi cultivars, mainly consisting of sugars, amino acids, and organic acids. Changes in the carbon metabolism of pakchoi leaves under amino acid treatments occurred via the accumulation of fructose, glucose, xylose, and arabinose. Disruption of amino acid metabolism resulted in accumulation of endogenous Gly in Gly treatment, Pro in Ala treatment, and Asn in three amino acid (Gly, Gln and Ala) treatments. By contrast, the levels of endogenous Gln and Leu decreased. However, this reduction varied among cultivars and amino acid types. Amino acid-N supply also affected the citric acid cycle, namely, the second stage of respiration, where leaves in Gly, Gln and Ala treatments contained low levels of malic, citric and succinic acids compared with leaves in NO3-N treatments. No significant difference in the metabolic responses was observed between the two cultivars which differed in their capability to use N. The response of primary metabolites in pakchoi leaves to amino acid-N supply may serve an important function in pakchoi adaptation to amino acid-N sources.
基金funded by the National High Technol-ogy Research and Development Program of China (863 Program,2006AA10Z221)China Postdoctoral Science Foundation (2005038436)+1 种基金Shanghai Leading Academic Discipline Project (B209)National Key Technologies R&D Program of China during the 11th Five-Year Plan period (2008BADA7B00 2008BADA7B01)
文摘Utilization of organic nitrogen (N) is an important aspect of plant N assimilation and has potential application in sustainable agriculture. The aim of this study was to investigate the plant growth, C and N accumulation in leaves and roots of tomato seedlings in response to inorganic (NH4^+-N, NO3^-N) and organic nitrogen (Gly-N). Different forms of nitrogen (NH4^+-N, NO3^--N, Gly-N) were supplied to two tomato cultivars (Shenfen 918 and Huying 932) using a hydroponics system. The plant dry biomass, chlorophyll content, root activity, total carbon and nitrogen content in roots and leaves, and total N absorption, etc. were assayed during the cultivation. Our results showed that no significant differences in plant height, dry biomass, and total N content were found within the first 16 d among three treatments; however, significant differences in treatments on 24 d and 32 d were observed, and the order was NO3^--N 〉 Gly-N 〉 NH4^+-N. Significant differences were also observed between the two tomato cultivars. Chlorophyll contents in the two cultivars were significantly increased by the Gly-N treatment, and root activity showed a significant decrease in NHa^+-N treatment. Tomato leaf total carbon content was slightly affected by different N forms; however, total carbon in root and total nitrogen in root and leaf were promoted significantly by inorganic and organic N. Among the applied N forms, the increasing effects of the NH4^+-N treatment were larger than that of the Gly-N. In a word, different N resources resulted in different physiological effects in tomatoes. Organic nitrogen (e.g., Gly-N) can be a proper resource of plant N nutrition. Tomatoes of different genotypes had different responses under organic nitrogen (e.g., Gly-N) supplies.
基金supported by grants from the National Natural Science Foundation of China(31372079)the National High Technology Research and Development Program of China(863 Program,2012AA101903)+1 种基金the Natural Science Foundation of Shanghai Science and Technology Committee,China(13ZR1422400)Shanghi Graduate Education and Innovation Program(Horticulture),China
文摘Salinity is one of the most important abiotic stresses that adversely affects crop growth and productivity. A subtractive suppression hybridization (SSH) library were constructed from the roots of salt-sensitive Yulu cultivar melon seedlings under salt stress; 557 high-quality expressed sequence tags (ESTs) were randomly sequenced, with an average size of 428 bp, which assembled into 68 contigs and 315 singletons. Compared with our previous SSH library generated from the salt-tolerant Bingxuecui cultivar, the proportion of transcripts involved in metabolism, protein fate, cellular communication/signal transduction mechanisms, and cell rescue/defense were 4, 1.46, 0.94, and 0.4% higher, respectively, in the salt-tolerant cultivar than the in salt-sensitive cultivar. Quantitative real-time PCR analysis of eleven transcripts revealed temporal variations in their expression in the two cultivars under salt stress. One NAC gene (JZ477011) was heterologously expressed in yeast for functional characterization, and enhanced the sensitivity of yeast cells to high-salinity to salt stress and inhibited their growth. Information regards to their functions would aid in the understanding of response mechanisms to saline stress and in the development of molecular markers for selecting salt-tolerant melon cultivars.