Objective: To study whether the ethanol extract of Phellinus merrillii(EPM) has chemopreventive potential against liver carcinogenesis. Methods: Thirty male Spraque-Dawley rats were randomly divided into control g...Objective: To study whether the ethanol extract of Phellinus merrillii(EPM) has chemopreventive potential against liver carcinogenesis. Methods: Thirty male Spraque-Dawley rats were randomly divided into control group, EPM control group, hepatocarcinoma control group, low-dose EPM group and high-dose EPM group, 6 in each group. Using the Solt and Farber protocol in a rat model of hepatocarcinogenesis, the chemopreventive effect of EPM on diethylnitrosamine(DEN)-initiated, 2-acetylaminofluorene(2-AAF) and partial hepatectomy(PH)-promoted liver carcinogenesis in rats was evaluated. Basic pathophysiological and histological examinations, together with the serum levels of glutamic oxaloacetic transaminase(s GOT), glutamic pyruvic transaminase(s GPT) and gamma-glutamyl transpeptidase(γ-GT) were measured. Results: Treatment of EPM at the concentration of 2 g/kg body weight in the diet for 8 weeks clearly prevented the development of carcinogenesis and reduced the levels of s GOT, s GPT, and serum γ-GT of rats as compared with the hepatocarcinoma control group(P〈0.05 or P〈0.01). These phenotypes were accompanied by a significant increase in natural killer cell activity. Conclusion: EPM showed a strong liver preventive effect against DEN+2-AAF+PH-induced hepatocarcinogenesis in a rat model.展开更多
基金Supported by China Medical University Tsuzuki Institute for Traditional Medicine(No.CMU95-PH-11 and No.CMU97-232)the National Science Council(No.NSC 95-2320-B-182-059-MY3)Chang Gung University(No.CMRPD160271),Taiwan,China
文摘Objective: To study whether the ethanol extract of Phellinus merrillii(EPM) has chemopreventive potential against liver carcinogenesis. Methods: Thirty male Spraque-Dawley rats were randomly divided into control group, EPM control group, hepatocarcinoma control group, low-dose EPM group and high-dose EPM group, 6 in each group. Using the Solt and Farber protocol in a rat model of hepatocarcinogenesis, the chemopreventive effect of EPM on diethylnitrosamine(DEN)-initiated, 2-acetylaminofluorene(2-AAF) and partial hepatectomy(PH)-promoted liver carcinogenesis in rats was evaluated. Basic pathophysiological and histological examinations, together with the serum levels of glutamic oxaloacetic transaminase(s GOT), glutamic pyruvic transaminase(s GPT) and gamma-glutamyl transpeptidase(γ-GT) were measured. Results: Treatment of EPM at the concentration of 2 g/kg body weight in the diet for 8 weeks clearly prevented the development of carcinogenesis and reduced the levels of s GOT, s GPT, and serum γ-GT of rats as compared with the hepatocarcinoma control group(P〈0.05 or P〈0.01). These phenotypes were accompanied by a significant increase in natural killer cell activity. Conclusion: EPM showed a strong liver preventive effect against DEN+2-AAF+PH-induced hepatocarcinogenesis in a rat model.