Objective:To observe the clinical efficacy of the Zhuang medicine Yingbupu decoction on stage I and II acute kidney injury through sequential test.Methods:The open one-way qualitative response sequential design of exp...Objective:To observe the clinical efficacy of the Zhuang medicine Yingbupu decoction on stage I and II acute kidney injury through sequential test.Methods:The open one-way qualitative response sequential design of experiments was adopted,and the patients with AKI in phase I and II who met the inclusion criteria were divided into the treatment group and the control group according to the order of hospitalization by random number table.On the basis of basic treatment,the treatment group was treated with Zhuang medicine Yingbupu decoction,and the control group was treated with Jinshuibao tablet.The clinical efficacy,TCM syndrome score,24 h urine volume,serum creatinine(Scr),microalbumin in urine(mAlb),neutrophil Gelatinase related lipid delivery albumin(NGAL)of the two groups were compared,and the adverse reactions and complications of the two groups were observed.Results:After 14 d of treatment,when the treatment group reached the 10th case,the experimental line contacted the upper bound U-line and reached the experimental standard to terminate the experiment.The effective hypothesis was accepted,and it was believed that the Zhuang medicine Yingbupu decoction had a therapeutic effect on stage I and II AKI.The conclusion was drawn that the treatment group received the Zhuang medicine Yingbupu.The clinical effective rate and improvement days were similar between the two groups,and there was no significant difference(P>0.05).However,the integral value of traditional Chinese medicine syndrome in the treatment group was lower than that in the control group(P<0.05),After treatment,the Scr,mAlb,and NGAL levels of patients in both groups were lower than before treatment(P<0.05).After treatment,the Scr,mAlb,and NGAL values in the treatment group were significantly lower than those in the control group(P<0.05).After treatment,the 24-hour urine volume in both groups was higher than that before treatment,and the values in the treatment group were significantly higher than those in the control group(P<0.05).During the treatment period,there were no significant adverse reactions or complications in either group.Conclusion:The Zhuang medicine Yingbupu decoction is effective in treating stage I and II AKI,and the Zhuang medicine Yingbupu can significantly improve the symptoms and quality of life of patients with stage I and II AKI.Its improvement of renal function is better than that of Jinshuibao tablets,and its safety is good.展开更多
All-atom molecular dynamic simulations and 2D-NOESY spectra were used to study the conformations and hydrogen bonds of ACE inhibitory tripeptide Ile-Ile-Pro(IIP) in aqueous and DMSO solutions. RMSD, Dis, Rg and SASA w...All-atom molecular dynamic simulations and 2D-NOESY spectra were used to study the conformations and hydrogen bonds of ACE inhibitory tripeptide Ile-Ile-Pro(IIP) in aqueous and DMSO solutions. RMSD, Dis, Rg and SASA were adopted to characterize the properties of tripeptide Ile-Ile-Pro in the MD simulations. Interestingly, the tripeptide molecule IIP exhibited different behaviors in aqueous and DMSO solutions. In aqueous solution, IIP was very flexible. The conformation could shift between extended and folded states very quickly. However,in DMSO solution, more folded conformations were observed. The interesting phenomena were proved by 2D-NOESY spectra.展开更多
目的:观察补阳还五汤合三仁汤加减治疗早期糖尿病肾病(DN)脾肾亏虚兼湿热瘀血证的临床疗效及对糖脂代谢、氧化应激、炎症因子的影响,并探讨其作用机制。方法:将72例患者按随机数字表法分为对照组与治疗组,各36例。在常规治疗基础上,对...目的:观察补阳还五汤合三仁汤加减治疗早期糖尿病肾病(DN)脾肾亏虚兼湿热瘀血证的临床疗效及对糖脂代谢、氧化应激、炎症因子的影响,并探讨其作用机制。方法:将72例患者按随机数字表法分为对照组与治疗组,各36例。在常规治疗基础上,对照组口服氯沙坦钾片,50 mg/次,1次/d。治疗组在对照组治疗的基础上给予补阳还五汤合三仁汤加减内服,1剂/d。两组疗程均为连续治疗3个月。观察两组患者临床疗效,比较两组患者治疗前后24 h尿微量白蛋白排泄率(UAER),血肌酐(SCr),血清胱抑素C(Cys C),尿素氮(BUN),空腹血糖(FBG),餐后2 h血糖(2 h PG),糖化血红蛋白(Hb A1c),总胆固醇(TC),甘油三酯(TG),低密度脂蛋白胆固醇(LDL-C),高密度脂蛋白胆固醇(HDL-C),丙二醛(MDA),超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-Px),白细胞介素-2(IL-2),白细胞介素-4(IL-4),白细胞介素-8(IL-8),白细胞介素^-10(IL^-10),肿瘤坏死因子-α(TNF-α)水平和安全性指标。结果:治疗组临床总有效率为88.9%,对照组61.1%,治疗组优于对照组(P<0.05)。两组治疗后UAER,SCr,Cys C,BUN水平较本组治疗前均降低(P<0.05);且治疗组均明显低于对照组(P<0.05)。两组治疗后FBG,2 h PG和Hb A1c水平较本组治疗前均降低(P<0.05);治疗后与对照组比较,两组FBG,2 h PG和Hb A1c水平比较差异无统计学意义。两组治疗后HDL-C水平较本组治疗前均升高(P<0.05);且治疗组明显高于对照组(P<0.05)。两组治疗后TC,TG和LDL-C,MDA水平较本组治疗前均降低(P<0.05);且治疗组均明显低于对照组(P<0.05)。两组治疗后MDA水平较本组治疗前均降低(P<0.05);且治疗组明显低于对照组(P<0.05)。两组治疗后血清IL-2,IL-8和TNF-α水平较本组治疗前均降低(P<0.05);且治疗组均明显低于对照组(P<0.05)。两组治疗后血清IL-4和IL^-10水平较本组治疗前均升高(P<0.05);且治疗组均明显高于对照组(P<0.05)。结论:补阳还五汤合三仁汤加减治疗早期DN脾肾亏虚兼湿热瘀血证的临床疗效显著且安全性好,能够进一步改善早期DN患者的肾功能、脂质代谢,抑制氧化应激反应及调节炎症因子分泌平衡。展开更多
基金2021 Guangxi Famous Traditional Chinese Medicine Inheritance Studio Construction Project[Guizhong Medicine Science and Education Development(2021)No.6]Key Discipline of Personnel Training-Ethnic Medicine(Zhuang Medicine)(050220250102)Graduate Student Innovation and Entrepreneurship Project of Guangxi University of Traditional Chinese Medicine in 2021(YCXJ2021125)。
文摘Objective:To observe the clinical efficacy of the Zhuang medicine Yingbupu decoction on stage I and II acute kidney injury through sequential test.Methods:The open one-way qualitative response sequential design of experiments was adopted,and the patients with AKI in phase I and II who met the inclusion criteria were divided into the treatment group and the control group according to the order of hospitalization by random number table.On the basis of basic treatment,the treatment group was treated with Zhuang medicine Yingbupu decoction,and the control group was treated with Jinshuibao tablet.The clinical efficacy,TCM syndrome score,24 h urine volume,serum creatinine(Scr),microalbumin in urine(mAlb),neutrophil Gelatinase related lipid delivery albumin(NGAL)of the two groups were compared,and the adverse reactions and complications of the two groups were observed.Results:After 14 d of treatment,when the treatment group reached the 10th case,the experimental line contacted the upper bound U-line and reached the experimental standard to terminate the experiment.The effective hypothesis was accepted,and it was believed that the Zhuang medicine Yingbupu decoction had a therapeutic effect on stage I and II AKI.The conclusion was drawn that the treatment group received the Zhuang medicine Yingbupu.The clinical effective rate and improvement days were similar between the two groups,and there was no significant difference(P>0.05).However,the integral value of traditional Chinese medicine syndrome in the treatment group was lower than that in the control group(P<0.05),After treatment,the Scr,mAlb,and NGAL levels of patients in both groups were lower than before treatment(P<0.05).After treatment,the Scr,mAlb,and NGAL values in the treatment group were significantly lower than those in the control group(P<0.05).After treatment,the 24-hour urine volume in both groups was higher than that before treatment,and the values in the treatment group were significantly higher than those in the control group(P<0.05).During the treatment period,there were no significant adverse reactions or complications in either group.Conclusion:The Zhuang medicine Yingbupu decoction is effective in treating stage I and II AKI,and the Zhuang medicine Yingbupu can significantly improve the symptoms and quality of life of patients with stage I and II AKI.Its improvement of renal function is better than that of Jinshuibao tablets,and its safety is good.
基金Supported by the National Natural Science Foundation of China(No.20903026)the Talents Introduction Foundation for Universities of Guangdong Province(2011)Scientific Research Foundation of the Natural Science Foundation of Guangdong Province(No.S2011010002483)
文摘All-atom molecular dynamic simulations and 2D-NOESY spectra were used to study the conformations and hydrogen bonds of ACE inhibitory tripeptide Ile-Ile-Pro(IIP) in aqueous and DMSO solutions. RMSD, Dis, Rg and SASA were adopted to characterize the properties of tripeptide Ile-Ile-Pro in the MD simulations. Interestingly, the tripeptide molecule IIP exhibited different behaviors in aqueous and DMSO solutions. In aqueous solution, IIP was very flexible. The conformation could shift between extended and folded states very quickly. However,in DMSO solution, more folded conformations were observed. The interesting phenomena were proved by 2D-NOESY spectra.
文摘目的:观察补阳还五汤合三仁汤加减治疗早期糖尿病肾病(DN)脾肾亏虚兼湿热瘀血证的临床疗效及对糖脂代谢、氧化应激、炎症因子的影响,并探讨其作用机制。方法:将72例患者按随机数字表法分为对照组与治疗组,各36例。在常规治疗基础上,对照组口服氯沙坦钾片,50 mg/次,1次/d。治疗组在对照组治疗的基础上给予补阳还五汤合三仁汤加减内服,1剂/d。两组疗程均为连续治疗3个月。观察两组患者临床疗效,比较两组患者治疗前后24 h尿微量白蛋白排泄率(UAER),血肌酐(SCr),血清胱抑素C(Cys C),尿素氮(BUN),空腹血糖(FBG),餐后2 h血糖(2 h PG),糖化血红蛋白(Hb A1c),总胆固醇(TC),甘油三酯(TG),低密度脂蛋白胆固醇(LDL-C),高密度脂蛋白胆固醇(HDL-C),丙二醛(MDA),超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-Px),白细胞介素-2(IL-2),白细胞介素-4(IL-4),白细胞介素-8(IL-8),白细胞介素^-10(IL^-10),肿瘤坏死因子-α(TNF-α)水平和安全性指标。结果:治疗组临床总有效率为88.9%,对照组61.1%,治疗组优于对照组(P<0.05)。两组治疗后UAER,SCr,Cys C,BUN水平较本组治疗前均降低(P<0.05);且治疗组均明显低于对照组(P<0.05)。两组治疗后FBG,2 h PG和Hb A1c水平较本组治疗前均降低(P<0.05);治疗后与对照组比较,两组FBG,2 h PG和Hb A1c水平比较差异无统计学意义。两组治疗后HDL-C水平较本组治疗前均升高(P<0.05);且治疗组明显高于对照组(P<0.05)。两组治疗后TC,TG和LDL-C,MDA水平较本组治疗前均降低(P<0.05);且治疗组均明显低于对照组(P<0.05)。两组治疗后MDA水平较本组治疗前均降低(P<0.05);且治疗组明显低于对照组(P<0.05)。两组治疗后血清IL-2,IL-8和TNF-α水平较本组治疗前均降低(P<0.05);且治疗组均明显低于对照组(P<0.05)。两组治疗后血清IL-4和IL^-10水平较本组治疗前均升高(P<0.05);且治疗组均明显高于对照组(P<0.05)。结论:补阳还五汤合三仁汤加减治疗早期DN脾肾亏虚兼湿热瘀血证的临床疗效显著且安全性好,能够进一步改善早期DN患者的肾功能、脂质代谢,抑制氧化应激反应及调节炎症因子分泌平衡。
