目的基于临床病例比较2019年欧洲抗风湿病联盟/美国风湿病学会(European League Against Rheumatism/American College of Rheumatology,EULAR/ACR)、2012年系统性红斑狼疮国际合作临床组织(Systemic Lupus International Collaborating...目的基于临床病例比较2019年欧洲抗风湿病联盟/美国风湿病学会(European League Against Rheumatism/American College of Rheumatology,EULAR/ACR)、2012年系统性红斑狼疮国际合作临床组织(Systemic Lupus International Collaborating Clinics,SLICC)及1997年美国风湿病学会(American College of Rheumatology,ACR)系统性红斑狼疮(systemic lupus erythematoses,SLE)分类标准间的差异并评估不同分类标准的诊断价值。方法收集2014年1月至2019年12月于贵州医科大学附属医院确诊为SLE病例73例,以其他结缔组织病82例为对照,统计患者各项临床指标及免疫学指标的数据,比较不同分类标准间灵敏度、特异度及ROC曲线下面积的差异。结果EULAR/ACR-2019、SLICC-2012及ACR-1997灵敏度及特异度分别为95.89%、98.63%、73.97%及92.68%、84.15%、98.78%,灵敏度及特异度在三种不同分类标准间存在显著差异,其中ACR-1997灵敏度最低,SLICC-2012特异度最低(P<0.001)。EULAR/ACR-2019、SLICC-2012、ACR-1997之间漏诊率、误诊率相比差异有统计学意义,其中ACR-1997漏诊率最高,但误诊率最低(P<0.05)。EULAR/ACR-2019、SLICC-2012、ACR-1997的ROC曲线下面积(AUC)分别为0.943、0.914、0.864。EULAR/ACR-2019与ACR-1997间AUC差异有统计学意义(P=0.0044),但SLICC-2012与EULAR/ACR-2019及ACR-1997之间AUC的差异无统计学意义(P>0.005)。结论EULAR/ACR-2019的SLE诊断价值优于ACR-1997,但SLICC-2012与EULAR/ACR-2019、ACR-1997相比无明显优势。将EULAR/ACR-2019和SLICC-2012分类标准与临床诊断相结合可能更有助于SLE的早期诊断,并指导治疗。展开更多
Limited genetic information is available concerning the polymorphisms of HIV-1 resistant genes in indigenous Chinese populations. The aim of this study is to identify the allelic frequencies of the chemokine and chemo...Limited genetic information is available concerning the polymorphisms of HIV-1 resistant genes in indigenous Chinese populations. The aim of this study is to identify the allelic frequencies of the chemokine and chemokine receptor genes in the Chinese mainland. Genomic DNA samples extracted from whole blood of 2318 subjects were analyzed by using PCR or PCR/restriction fragment length polymorphism (RFLP) assays, and further confirmed by direct DNA sequencing. Higher frequencies of mutant CCR2-64I (19.15%-28.79%) and SDF1-3’A (19.10% -29.86%) alleles were found in subjects of 8 ethnic groups in the Chinese mainland. In contrast, the △32 mutation in CCRS gene occurs at a very low frequency (0.0016, n=1287) in Han population. A relatively high frequency of CCR5-wt/△32 heterozygotes was observed in Uygurian and Mongolian populations. No A32 mutation allele was detected in Tibetan and other 4 ethnic groups in Yunnan Province. There was no CCR5-m303 mutation in subjects of any ethnic group in the Chinese展开更多
文摘目的基于临床病例比较2019年欧洲抗风湿病联盟/美国风湿病学会(European League Against Rheumatism/American College of Rheumatology,EULAR/ACR)、2012年系统性红斑狼疮国际合作临床组织(Systemic Lupus International Collaborating Clinics,SLICC)及1997年美国风湿病学会(American College of Rheumatology,ACR)系统性红斑狼疮(systemic lupus erythematoses,SLE)分类标准间的差异并评估不同分类标准的诊断价值。方法收集2014年1月至2019年12月于贵州医科大学附属医院确诊为SLE病例73例,以其他结缔组织病82例为对照,统计患者各项临床指标及免疫学指标的数据,比较不同分类标准间灵敏度、特异度及ROC曲线下面积的差异。结果EULAR/ACR-2019、SLICC-2012及ACR-1997灵敏度及特异度分别为95.89%、98.63%、73.97%及92.68%、84.15%、98.78%,灵敏度及特异度在三种不同分类标准间存在显著差异,其中ACR-1997灵敏度最低,SLICC-2012特异度最低(P<0.001)。EULAR/ACR-2019、SLICC-2012、ACR-1997之间漏诊率、误诊率相比差异有统计学意义,其中ACR-1997漏诊率最高,但误诊率最低(P<0.05)。EULAR/ACR-2019、SLICC-2012、ACR-1997的ROC曲线下面积(AUC)分别为0.943、0.914、0.864。EULAR/ACR-2019与ACR-1997间AUC差异有统计学意义(P=0.0044),但SLICC-2012与EULAR/ACR-2019及ACR-1997之间AUC的差异无统计学意义(P>0.005)。结论EULAR/ACR-2019的SLE诊断价值优于ACR-1997,但SLICC-2012与EULAR/ACR-2019、ACR-1997相比无明显优势。将EULAR/ACR-2019和SLICC-2012分类标准与临床诊断相结合可能更有助于SLE的早期诊断,并指导治疗。
文摘Limited genetic information is available concerning the polymorphisms of HIV-1 resistant genes in indigenous Chinese populations. The aim of this study is to identify the allelic frequencies of the chemokine and chemokine receptor genes in the Chinese mainland. Genomic DNA samples extracted from whole blood of 2318 subjects were analyzed by using PCR or PCR/restriction fragment length polymorphism (RFLP) assays, and further confirmed by direct DNA sequencing. Higher frequencies of mutant CCR2-64I (19.15%-28.79%) and SDF1-3’A (19.10% -29.86%) alleles were found in subjects of 8 ethnic groups in the Chinese mainland. In contrast, the △32 mutation in CCRS gene occurs at a very low frequency (0.0016, n=1287) in Han population. A relatively high frequency of CCR5-wt/△32 heterozygotes was observed in Uygurian and Mongolian populations. No A32 mutation allele was detected in Tibetan and other 4 ethnic groups in Yunnan Province. There was no CCR5-m303 mutation in subjects of any ethnic group in the Chinese
