The identification and localization of vertebrate follicle stimulating hormone (FSH) and luteinizing hormone (LH) in crustaceans may further elucidate the regulation mechanisms in arthropod repro-duction. Using im...The identification and localization of vertebrate follicle stimulating hormone (FSH) and luteinizing hormone (LH) in crustaceans may further elucidate the regulation mechanisms in arthropod repro-duction. Using immunocytochemical staining techniques, this study has localized vertebrate FSH-like and LH-like substances in neurons in the subesophageal and thoracic ganglia from the thoracic ganglion mass (TGM) of Scylla paramamosain (Crustacea: Decapoda: Brachyura). Enzyme-linked immunosorbent assay (ELISA) has shown that the concentrations of both FSH-and LH-like sub-stances increased markedly in the TGM during the vitellogenic stage compared with that in the previtellogenic stage. These results indicate that substances resembling the vertebrate FSH and LH are present in S. paramamosain, and they may be involved in the development of the ovary as well as in ovulation.展开更多
The regulation of three biogenic amines over the reproductive neuroendoerine activity of the male Scylla serrata was investigated by in vivo injection and in vitro incubation. The testicular index, the ratio of the ma...The regulation of three biogenic amines over the reproductive neuroendoerine activity of the male Scylla serrata was investigated by in vivo injection and in vitro incubation. The testicular index, the ratio of the mature sections in testes, and the ratio of Type B cells in androgenic gland were taken as the quantitative indexes. The in vivo injections indicated that: 5-HT can significantly promote the testicular development and the secretion of the androgenic gland in S. serrata; DA can inhibit the testicular development, but no influence on the secretion of the androgenic gland was found; no significant difference was observed between the OA-injected group and the concurrent control group. In vitro incubations showed that: 5-HT can stimulate the secretion of the brain and the thoracic ganglia, thus accelerating that of the androgenic gland; however, neither OA nor DA showed any significant influence on the secretion of the brain and the thoracic ganglionic mass. As to the optic ganglia, the three biogenic amines hardly have any effect on its secretion. It is the first time to report the regulation of biogenic amines over the reproductive neuroendocrine of male crustaceans through vitro experiments. Results corroborate that 5-HT activates the brain and the thoracic ganglia to secret GSH first, then promote the testicular development through the activity of the androgenic gland.展开更多
基金The National Natural Science Foundation of China under contract Nos 40406030 and 40776084the National High Technology Research and Development Program of China under contract No 2006AA10A406the Program for New Century Excellent Talents in Fujian Province University
文摘The identification and localization of vertebrate follicle stimulating hormone (FSH) and luteinizing hormone (LH) in crustaceans may further elucidate the regulation mechanisms in arthropod repro-duction. Using immunocytochemical staining techniques, this study has localized vertebrate FSH-like and LH-like substances in neurons in the subesophageal and thoracic ganglia from the thoracic ganglion mass (TGM) of Scylla paramamosain (Crustacea: Decapoda: Brachyura). Enzyme-linked immunosorbent assay (ELISA) has shown that the concentrations of both FSH-and LH-like sub-stances increased markedly in the TGM during the vitellogenic stage compared with that in the previtellogenic stage. These results indicate that substances resembling the vertebrate FSH and LH are present in S. paramamosain, and they may be involved in the development of the ovary as well as in ovulation.
基金supported by the National Natural Science Foundation of China under contract No.30300269the National High Technology Research and Development Program("863"program)of China under contract No.2002AA603013.
文摘The regulation of three biogenic amines over the reproductive neuroendoerine activity of the male Scylla serrata was investigated by in vivo injection and in vitro incubation. The testicular index, the ratio of the mature sections in testes, and the ratio of Type B cells in androgenic gland were taken as the quantitative indexes. The in vivo injections indicated that: 5-HT can significantly promote the testicular development and the secretion of the androgenic gland in S. serrata; DA can inhibit the testicular development, but no influence on the secretion of the androgenic gland was found; no significant difference was observed between the OA-injected group and the concurrent control group. In vitro incubations showed that: 5-HT can stimulate the secretion of the brain and the thoracic ganglia, thus accelerating that of the androgenic gland; however, neither OA nor DA showed any significant influence on the secretion of the brain and the thoracic ganglionic mass. As to the optic ganglia, the three biogenic amines hardly have any effect on its secretion. It is the first time to report the regulation of biogenic amines over the reproductive neuroendocrine of male crustaceans through vitro experiments. Results corroborate that 5-HT activates the brain and the thoracic ganglia to secret GSH first, then promote the testicular development through the activity of the androgenic gland.