Detecting exogenous gene copy numbers is crucial for examining genetically modified organisms. Traditional methods face a trade-off between feasibility and cost, highlighting the need for a high-precision and low-cost...Detecting exogenous gene copy numbers is crucial for examining genetically modified organisms. Traditional methods face a trade-off between feasibility and cost, highlighting the need for a high-precision and low-cost alternative. In this study, we introduced the RISE method, which is based on Rare Allele Infusion and Sanger Sequencing Estimation, for rice.展开更多
基金supported by the Natural Science Foundation of Hunan Province for Distinguished Young Scholars, China (Grant No.2021JJ10041)the Biological Breeding-National Science and Technology Major Project, China (Grant No.2023ZD04072)the Hainan Yazhou Bay Seed Laboratory and National Seed Group, China (Grant Nos.B23CQ15HP and B23YQ1517)。
文摘Detecting exogenous gene copy numbers is crucial for examining genetically modified organisms. Traditional methods face a trade-off between feasibility and cost, highlighting the need for a high-precision and low-cost alternative. In this study, we introduced the RISE method, which is based on Rare Allele Infusion and Sanger Sequencing Estimation, for rice.