该研究考察紫斑牡丹花、叶的抗菌活性，筛选其抗菌主要活性成分，预测抗菌靶点，探讨其多成分、多靶点抗菌作用机制。首先，采用微量稀释法测定甲醇、乙酸乙酯提取紫斑牡丹花、叶对金黄色葡萄球菌、表皮葡萄球菌、枯草芽孢杆菌、蜡样芽...该研究考察紫斑牡丹花、叶的抗菌活性，筛选其抗菌主要活性成分，预测抗菌靶点，探讨其多成分、多靶点抗菌作用机制。首先，采用微量稀释法测定甲醇、乙酸乙酯提取紫斑牡丹花、叶对金黄色葡萄球菌、表皮葡萄球菌、枯草芽孢杆菌、蜡样芽胞杆菌、大肠杆菌、肺炎克雷伯氏菌、铜绿假单胞菌的最小抑菌浓度（MIC）。其次，利用Uniprot数据库找到相对应的基因靶点，并通过RCSB网站寻找相对应的蛋白亚型的PDBID以及小分子结构为受体，前期已鉴定的紫斑牡丹花、叶化学成分以PubChem等数据库进行结构确认，通过Chem Draw，Avogadro和Discovery Studio Visualizer软件进行配体的构建，采用QuickVina2．0软件进行分子对接。最后，借助Cytoscape3．5．1软件绘制活性成分与抗菌靶点网络图，使用Uniprot在线数据库进行靶点基因功能及代谢通路进行分析。实验结果表明紫斑牡丹花、叶提取物具有抗菌活性，紫斑牡丹花甲醇提取物具有最强的抗菌作用。网络分析表明紫斑牡丹花、叶中筛选得到活性成分29个，抗菌靶点18个。通过紫斑牡丹花、叶活性成分与抗菌靶点网络数的比较，预测其抗菌成分主要是黄酮和酚酸，抗菌机制主要是抑制细菌蛋白的合成。该研究通过体外抗菌实验和网络药理学的筛选，发现紫斑牡丹花、叶提取物具有抗菌活性，为紫斑牡丹花、叶抗菌机制研究提供新的思路和线索。展开更多
American ginseng(Panax quinquefolius L.) is a well-known Asian traditional herbal medicine with a large market demand. The plant is native to eastern North America, and its main producing areas worldwide are decreasin...American ginseng(Panax quinquefolius L.) is a well-known Asian traditional herbal medicine with a large market demand. The plant is native to eastern North America, and its main producing areas worldwide are decreasing due to continuous cropping obstacles and environmental changes. Therefore, the identification of maximum similarities of new ecological distribution of P.quinquefolius, and prediction of its response to climate change in the future are necessary for plant introduction and cultivation. In this study, the areas with potential ecological suitability for P. quinquefolius were predicted using the geographic information system for global medicinal plants(GMPGIS) based on 476 occurrence points and 19 bioclimatic variables. The results indicate that the new ecologically suitable areas for P. quinquefolius are East Asia and the mid-eastern Europe, which are mainly distributed in China, Russia, Japan, Ukraine, Belarus, North Korean, South Korea, and Romania. Under global climate change scenarios, the suitable planting areas for P. quinquefolius would be increased by 9.16%–30.97%, and expanding north and west over the current ecologically suitable areas by 2070. The potential increased areas that are ecologically suitable include northern Canada, Eastern Europe, and the Lesser Khingan Mountains of China, and reduced regions are mainly in central China, the southern U.S., and southern Europe. Jackknife tests indicate that the precipitation of the warmest quarter was the important climatic factor controlling the distribution of P. quinquefolius.Our findings can be used as a useful guide for P. quinquefolius introduction and cultivation in ecologically suitable areas.展开更多
The adulteration of herbal products is a threat to consumer safety. In the present study, we surveyed the species composition of commercial Radix Clerodendri Japonicum products using DNA barcoding as a supervisory met...The adulteration of herbal products is a threat to consumer safety. In the present study, we surveyed the species composition of commercial Radix Clerodendri Japonicum products using DNA barcoding as a supervisory method. A reference database for plant-material DNA-barcode was successfully constructed with 48 voucher samples from 12 Clerodendrum species. The database was used to identify 27 Radix Clerodendri Japonicum decoction piece samples purchased from drug stores and hospitals. The DNA sequencing results revealed that only 1 decoction piece(3.70%) was authentic C. japonicum, as recorded in the Dai Pharmacopeia, whereas the other samples were all adulterants, indicating a potential safety issue. The results indicate that decoction pieces that are available in the market have complex origins and that DNA barcoding is a suitable tool for regulation of Dai medicines.展开更多
Cancer is one of the most devastating diseases worldwide and definitive therapeutics for treating cancer are not yet available despite extensive research efforts. The key challenges include limiting factors connected ...Cancer is one of the most devastating diseases worldwide and definitive therapeutics for treating cancer are not yet available despite extensive research efforts. The key challenges include limiting factors connected with traditional chemotherapeutics, primarily drug resistance, low response rates, and adverse side-effects. Therefore, there is a high demand for novel anti-cancer drugs that are both potent and safe for cancer prevention and treatment. Gallic acid(GA), a natural botanic phenolic compound, can mediate various therapeutic properties that are involved in anti-inflammation, anti-obesity, and anti-cancer activities. More recently, GA has been shown to exert anti-cancer activities via several biological pathways that include migration, metastasis, apoptosis, cell cycle arrest, angiogenesis, and oncogene expression. This review discusses two aspects, one is the anti-cancer potential of GA against different types of cancer and the underlying molecular mechanisms, the other is the bibliometric analysis of GA in cancer and tumor research. The results indicated that lung cancer, prostate cancer, stomach cancer, and colon adenocarcinoma may become a hot topic in further research. Overall, this review provides evidence that GA represents a promising novel, potent, and safe anti-cancer drug candidate for treating cancer.展开更多
A rapid and validated UPLC-MS method was developed for investigating the absorbed components of Paederia scandens(Lour.) Merrill(P.scandens) in rat plasma.The bioactive constituents in plasma samples from rats adminis...A rapid and validated UPLC-MS method was developed for investigating the absorbed components of Paederia scandens(Lour.) Merrill(P.scandens) in rat plasma.The bioactive constituents in plasma samples from rats administrated orally with P.scandens extract were analyzed by Ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS).Four prototype compounds were identified in rat serum as potential bioactive components of P.scandens by comparing their retention times and mass spectrometry data or by mass spectrometry analysis and retrieving the reference literatures.Glucuronidation after deglycosylation was the major metabolic pathway for the iridoid glycosides in P.scandens.These results showed that the methods had high sensitivity and resolution and were suitable for identifying the bioactive constituents in plasma after oral administration of P.scandens.providing helpful chemical information for further pharmacological and mechanistic researched on the P.scandens.展开更多
Objective To analyze the volatile oil from the branches of Picrasma quassioides. Methods Volatile oil was extracted from the branches of P. quassioides by steam distillation. GC-MS method was used to analyze the compo...Objective To analyze the volatile oil from the branches of Picrasma quassioides. Methods Volatile oil was extracted from the branches of P. quassioides by steam distillation. GC-MS method was used to analyze the components. Results A total of 49 compounds were isolated, and 46 compounds were successfully identified, which represented over 98% of the total oil composition. The major components of the volatile oil in the branches of P. quassioides included caryophyllene (12.83%), 12-oxabicyclo[9.1.0]dodeca-3,7-diene,1,5,5,8-tetramethyl-[1R-(1R*,3E,7E,11-R*)] (12.29%), 1-hexanol (9.96%), naphthalene, 1,2,3,5,6,7,8,8a-octahydro-1,8a-dimethyl-7-(1-methyletheyl)-[1S-(1a,7a,8aa)] (7.32%), aromadendrene oxide-(2) (6.69%), and α-caryophyllene (3.88%). Conclusion The major components in volatile oil are terpenoids, hydroxy compounds, and other acyclic alkane compounds.展开更多
Hypericin is one of the most important phenanthoperylene quinones extracted mainly from plants of the genus Hypericum belonging to the sections Euhypericum and Campylosporus of Keller's classification. Widespread ...Hypericin is one of the most important phenanthoperylene quinones extracted mainly from plants of the genus Hypericum belonging to the sections Euhypericum and Campylosporus of Keller's classification. Widespread attention to the antiviral and anti-tumor properties of hypericin has spurred investigations of the chemical synthesis and biosynthesis of this unique compound. However, the synthetic strategies are challenging for organic and biological chemists. In this review, specific significant advances in total synthesis, semi-synthesis, and biosynthesis in the past decades are summarized.展开更多
Objective:To investigate the current status and further dtevelopment of Panax genus and6 important individual species including P.notoginseng,P.quinquefolium,P.vietnamensis,P.japonicus,P.stipuleanatus and P.zingiberen...Objective:To investigate the current status and further dtevelopment of Panax genus and6 important individual species including P.notoginseng,P.quinquefolium,P.vietnamensis,P.japonicus,P.stipuleanatus and P.zingiberensis.Methods:The bibliometric analysis was based on the Web of Science core database platform from Thomson Reuters.Totally,7,574 records of scientific research of Panax species published from 1900—2019 were analyzed.The statistical and visualization analysis was performed by CiteSpace and HistCite software.Results:The academic research of Panax species increase promptly.Plant science is the main research field while research and experimental medicine and agricultural engineering will be the further development tendency.Particularly,the discrimination research of P.notoginseng will be the research tendency among Panax species,especially diversity research.In addition,P.vietnamensis deserves more attention in the genus Panax.Conclusion:This research provides a reference for further research of the genus and individual species.展开更多
文摘目的明确肉苁蓉属药用植物叶绿体基因组密码子使用偏性及影响因素。方法以肉苁蓉Cistanche deserticola、盐生肉苁蓉C.salsa、沙苁蓉C.sinensis和管花肉苁蓉C.tubulosa为研究材料,利用CUSP在线软件、CodonW 1.4.2、SPSS、Microsoft Excel等软件分析其基因密码子使用偏性参数。结果4个肉苁蓉属植物叶绿体基因组的密码子使用模式相似,密码子均以第3位碱基A/T结尾且密码子使用更偏向A/T碱基;4个物种的有效密码子数值(effective number of codon,ENC)均大于35,说明肉苁蓉属物种叶绿体基因密码子偏好性较弱。中性绘图分析、有效密码字数(ENC-plot)分析、奇偶偏好性(PR2-plot)分析和对应性分析的结果均说明自然选择是影响肉苁蓉属叶绿体基因组密码子偏好性的主要因素。利用同义密码子的相对使用频率(relative synonymous codon usage,RSCU)值筛选出肉苁蓉属4个物种共同拥有4个最优密码子。结论对肉苁蓉属4个物种叶绿体基因组密码子使用偏性进行分析,揭示了影响其密码子偏好性的影响因素,为进一步探讨肉苁蓉属植物的系统进化、环境适应性和品种改良提供了理论基础。
文摘该研究考察紫斑牡丹花、叶的抗菌活性，筛选其抗菌主要活性成分，预测抗菌靶点，探讨其多成分、多靶点抗菌作用机制。首先，采用微量稀释法测定甲醇、乙酸乙酯提取紫斑牡丹花、叶对金黄色葡萄球菌、表皮葡萄球菌、枯草芽孢杆菌、蜡样芽胞杆菌、大肠杆菌、肺炎克雷伯氏菌、铜绿假单胞菌的最小抑菌浓度（MIC）。其次，利用Uniprot数据库找到相对应的基因靶点，并通过RCSB网站寻找相对应的蛋白亚型的PDBID以及小分子结构为受体，前期已鉴定的紫斑牡丹花、叶化学成分以PubChem等数据库进行结构确认，通过Chem Draw，Avogadro和Discovery Studio Visualizer软件进行配体的构建，采用QuickVina2．0软件进行分子对接。最后，借助Cytoscape3．5．1软件绘制活性成分与抗菌靶点网络图，使用Uniprot在线数据库进行靶点基因功能及代谢通路进行分析。实验结果表明紫斑牡丹花、叶提取物具有抗菌活性，紫斑牡丹花甲醇提取物具有最强的抗菌作用。网络分析表明紫斑牡丹花、叶中筛选得到活性成分29个，抗菌靶点18个。通过紫斑牡丹花、叶活性成分与抗菌靶点网络数的比较，预测其抗菌成分主要是黄酮和酚酸，抗菌机制主要是抑制细菌蛋白的合成。该研究通过体外抗菌实验和网络药理学的筛选，发现紫斑牡丹花、叶提取物具有抗菌活性，为紫斑牡丹花、叶抗菌机制研究提供新的思路和线索。
基金supported by the National Natural Science Foundation of China(Nos.81403053 and 81274013)the National Key Research and Development Program of China(No.2017YFC1702100)+1 种基金the National Science-technology Support Plan Project(No.2015BAI05B02)China Postdoctoral Science Foundation(No.2017M611128)
文摘American ginseng(Panax quinquefolius L.) is a well-known Asian traditional herbal medicine with a large market demand. The plant is native to eastern North America, and its main producing areas worldwide are decreasing due to continuous cropping obstacles and environmental changes. Therefore, the identification of maximum similarities of new ecological distribution of P.quinquefolius, and prediction of its response to climate change in the future are necessary for plant introduction and cultivation. In this study, the areas with potential ecological suitability for P. quinquefolius were predicted using the geographic information system for global medicinal plants(GMPGIS) based on 476 occurrence points and 19 bioclimatic variables. The results indicate that the new ecologically suitable areas for P. quinquefolius are East Asia and the mid-eastern Europe, which are mainly distributed in China, Russia, Japan, Ukraine, Belarus, North Korean, South Korea, and Romania. Under global climate change scenarios, the suitable planting areas for P. quinquefolius would be increased by 9.16%–30.97%, and expanding north and west over the current ecologically suitable areas by 2070. The potential increased areas that are ecologically suitable include northern Canada, Eastern Europe, and the Lesser Khingan Mountains of China, and reduced regions are mainly in central China, the southern U.S., and southern Europe. Jackknife tests indicate that the precipitation of the warmest quarter was the important climatic factor controlling the distribution of P. quinquefolius.Our findings can be used as a useful guide for P. quinquefolius introduction and cultivation in ecologically suitable areas.
基金supported by National Natural Science Foundation of China(Grant No.31460084)training scheme of reserve talents for young and middle-aged academic and technical leaders of Yunnan province(Grant 2015HB058)
文摘The adulteration of herbal products is a threat to consumer safety. In the present study, we surveyed the species composition of commercial Radix Clerodendri Japonicum products using DNA barcoding as a supervisory method. A reference database for plant-material DNA-barcode was successfully constructed with 48 voucher samples from 12 Clerodendrum species. The database was used to identify 27 Radix Clerodendri Japonicum decoction piece samples purchased from drug stores and hospitals. The DNA sequencing results revealed that only 1 decoction piece(3.70%) was authentic C. japonicum, as recorded in the Dai Pharmacopeia, whereas the other samples were all adulterants, indicating a potential safety issue. The results indicate that decoction pieces that are available in the market have complex origins and that DNA barcoding is a suitable tool for regulation of Dai medicines.
基金Supported by the National Natural Science Foundation of China (No. U1812403-1-1)National Science and Technology Fundamental Resources Investigation Program of China (No. 2018FY100701)。
文摘Cancer is one of the most devastating diseases worldwide and definitive therapeutics for treating cancer are not yet available despite extensive research efforts. The key challenges include limiting factors connected with traditional chemotherapeutics, primarily drug resistance, low response rates, and adverse side-effects. Therefore, there is a high demand for novel anti-cancer drugs that are both potent and safe for cancer prevention and treatment. Gallic acid(GA), a natural botanic phenolic compound, can mediate various therapeutic properties that are involved in anti-inflammation, anti-obesity, and anti-cancer activities. More recently, GA has been shown to exert anti-cancer activities via several biological pathways that include migration, metastasis, apoptosis, cell cycle arrest, angiogenesis, and oncogene expression. This review discusses two aspects, one is the anti-cancer potential of GA against different types of cancer and the underlying molecular mechanisms, the other is the bibliometric analysis of GA in cancer and tumor research. The results indicated that lung cancer, prostate cancer, stomach cancer, and colon adenocarcinoma may become a hot topic in further research. Overall, this review provides evidence that GA represents a promising novel, potent, and safe anti-cancer drug candidate for treating cancer.
基金supported by the National Natural Science Foundation(Nos.81274013 and 81473315)the National Natural foundationof the major program of China(No.81130069)+1 种基金the Chinese National S&T Special Project on Major New Drug Innovation(No.2011ZX09307-002-01)the program for Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China(No.IRT1140)
文摘A rapid and validated UPLC-MS method was developed for investigating the absorbed components of Paederia scandens(Lour.) Merrill(P.scandens) in rat plasma.The bioactive constituents in plasma samples from rats administrated orally with P.scandens extract were analyzed by Ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS).Four prototype compounds were identified in rat serum as potential bioactive components of P.scandens by comparing their retention times and mass spectrometry data or by mass spectrometry analysis and retrieving the reference literatures.Glucuronidation after deglycosylation was the major metabolic pathway for the iridoid glycosides in P.scandens.These results showed that the methods had high sensitivity and resolution and were suitable for identifying the bioactive constituents in plasma after oral administration of P.scandens.providing helpful chemical information for further pharmacological and mechanistic researched on the P.scandens.
基金Chinese National S & T Special Project on Major New Drug Innovation (2011ZX09307-002-01)the Selected Program of Personnel Department for Oversea Scholar (2009-2011)+1 种基金the Natural Foundation of the Major Program of China (81130069 and 81274013)Key Technologies of the Quality Standards and the Safety of Health Food, 863 Program (2010AA023006)
文摘Objective To analyze the volatile oil from the branches of Picrasma quassioides. Methods Volatile oil was extracted from the branches of P. quassioides by steam distillation. GC-MS method was used to analyze the components. Results A total of 49 compounds were isolated, and 46 compounds were successfully identified, which represented over 98% of the total oil composition. The major components of the volatile oil in the branches of P. quassioides included caryophyllene (12.83%), 12-oxabicyclo[9.1.0]dodeca-3,7-diene,1,5,5,8-tetramethyl-[1R-(1R*,3E,7E,11-R*)] (12.29%), 1-hexanol (9.96%), naphthalene, 1,2,3,5,6,7,8,8a-octahydro-1,8a-dimethyl-7-(1-methyletheyl)-[1S-(1a,7a,8aa)] (7.32%), aromadendrene oxide-(2) (6.69%), and α-caryophyllene (3.88%). Conclusion The major components in volatile oil are terpenoids, hydroxy compounds, and other acyclic alkane compounds.
基金supported by the Key National Natural Science Foundation of China(No.81130069)Selected Program of Personnel Department for Oversea Scholars(2009-2011)+1 种基金the Chinese National S&T Special Project on Major New Drug Innovation(No.2011ZX09307-002-01)the Key Technologies of the Quality Standards and the Safety of Health Food,"863 Program"(No.2010AA023006)
文摘Hypericin is one of the most important phenanthoperylene quinones extracted mainly from plants of the genus Hypericum belonging to the sections Euhypericum and Campylosporus of Keller's classification. Widespread attention to the antiviral and anti-tumor properties of hypericin has spurred investigations of the chemical synthesis and biosynthesis of this unique compound. However, the synthetic strategies are challenging for organic and biological chemists. In this review, specific significant advances in total synthesis, semi-synthesis, and biosynthesis in the past decades are summarized.
基金Supported by Beijing Natural Scientific Foundation(No.7202135)National Natural Science Foundation of China(U1812403-1-1,No.81473315)+1 种基金National Science and Technology Fundamental Resources Investigation Program of China(No.2018FY100701)the Open Research Fund of Chengdu University of Traditional Chinese Medicine Key Laboratory of Systematic Research of Distinctive Chinese Medicine Resources in Southwest China(No.SCMR20210,2021GZ2011003)。
文摘Objective:To investigate the current status and further dtevelopment of Panax genus and6 important individual species including P.notoginseng,P.quinquefolium,P.vietnamensis,P.japonicus,P.stipuleanatus and P.zingiberensis.Methods:The bibliometric analysis was based on the Web of Science core database platform from Thomson Reuters.Totally,7,574 records of scientific research of Panax species published from 1900—2019 were analyzed.The statistical and visualization analysis was performed by CiteSpace and HistCite software.Results:The academic research of Panax species increase promptly.Plant science is the main research field while research and experimental medicine and agricultural engineering will be the further development tendency.Particularly,the discrimination research of P.notoginseng will be the research tendency among Panax species,especially diversity research.In addition,P.vietnamensis deserves more attention in the genus Panax.Conclusion:This research provides a reference for further research of the genus and individual species.
