目的通过干扰TGF-βRⅡ的表达,探讨TGF-βRⅡ对AngⅡ诱导GMC增殖的作用及对TGF-β1与psmad3表达的影响。方法 GMC转入荧光对照siRNA,6 h于荧光显微镜观察沉默效率。分别转入阴性对照siRNA与TGF-βRⅡsiRNA,24 h Western blot检测TGF-β...目的通过干扰TGF-βRⅡ的表达,探讨TGF-βRⅡ对AngⅡ诱导GMC增殖的作用及对TGF-β1与psmad3表达的影响。方法 GMC转入荧光对照siRNA,6 h于荧光显微镜观察沉默效率。分别转入阴性对照siRNA与TGF-βRⅡsiRNA,24 h Western blot检测TGF-βRⅡ的表达。实验分为:空白对照组(Con组),AngⅡ组、siRNA阴性对照组(NC组)、TGF-βRⅡsiRNA干扰组(沉默组)。除Con组外,AngⅡ刺激各组24 h,Western blot检验TGF-β1与psmad3的蛋白水平。CCK8试剂盒检测GMC增殖变化。结果 (1)转染TGF-βRⅡsiRNA后检测TGF-βRⅡ的蛋白水平表达明显降低,与对照组相比表达差异有统计学意义(P<0.05);(2)AngⅡ能刺激TGF-β1的表达,转染TGF-βRⅡsiRNA后,TGF-β1蛋白表达下降(P<0.05);(3)AngⅡ能促进smad3的磷酸化,转染TGF-βRⅡsiRNA后psmad3蛋白表达下降(P<0.05);(4)AngⅡ促进GMC增殖,转染TGF-βRⅡsiRNA后减轻AngⅡ的增殖作用(P<0.05)。结论 AngⅡ刺激细胞增殖与TGF-β1表达及smad3的磷酸化有关,且依赖TGF-βRⅡ的表达。展开更多
Renewable energy (RE) has been attached high attention around the world due to its carbon-free and indigenous production in a sustainable way. China enjoys plenty of renewable energy resources, particularly the wind, ...Renewable energy (RE) has been attached high attention around the world due to its carbon-free and indigenous production in a sustainable way. China enjoys plenty of renewable energy resources, particularly the wind, solar, hydro- and biomass energy, which could be a sound basis for a large-scale exploitation. This report examines the current status of RE technology and industry, analyzes the challenges of promoting RE in China. In order to pave the way for a long-term development of RE, this paper outlines the basic principles and priorities for individual RE technology. In line with these, the paper puts forward the RE targets and further describes the RE road map by 2020, 2030 and extend to 2050, taking consideration of China’s RE resources, industrial basis and energy demand etc. At last, this paper provides some recommendations to ensure the achievements of the RE targets.展开更多
On the foundation of nonlinear robust control and exact generator model, this paper presents a design principle of NR-PSS (Nonlinear Robust Power System Stabilizer) for multi-machine power system, based on which an in...On the foundation of nonlinear robust control and exact generator model, this paper presents a design principle of NR-PSS (Nonlinear Robust Power System Stabilizer) for multi-machine power system, based on which an industrial NR-PSS equipment is developed. For popularizing it, the proposed parameter setting method of NR-PSS is completely the same as the widely used parameter adjustment rule of PSS. By virtue of real time digital simulator (RTDS), large disturbance experiments are carried out to compare the performances between generator excitation system equipped with NR-PSS and PSS in order to verify the correctness of design theory. The results show that compared with classical PSS, the proposed NR-PSS can dramatically improve the generator damping and attenuate the oscillation much faster, enhance the generator damping and raise both the small signal and large disturbance transient stability transmission power limit remarkably. The NR-PSS equipment with independent intellectual property right has been successfully put into operation on a 300 MW generator in Baishan Hydro Plant of Northeast China Grid more than 10 months.展开更多
文摘目的通过干扰TGF-βRⅡ的表达,探讨TGF-βRⅡ对AngⅡ诱导GMC增殖的作用及对TGF-β1与psmad3表达的影响。方法 GMC转入荧光对照siRNA,6 h于荧光显微镜观察沉默效率。分别转入阴性对照siRNA与TGF-βRⅡsiRNA,24 h Western blot检测TGF-βRⅡ的表达。实验分为:空白对照组(Con组),AngⅡ组、siRNA阴性对照组(NC组)、TGF-βRⅡsiRNA干扰组(沉默组)。除Con组外,AngⅡ刺激各组24 h,Western blot检验TGF-β1与psmad3的蛋白水平。CCK8试剂盒检测GMC增殖变化。结果 (1)转染TGF-βRⅡsiRNA后检测TGF-βRⅡ的蛋白水平表达明显降低,与对照组相比表达差异有统计学意义(P<0.05);(2)AngⅡ能刺激TGF-β1的表达,转染TGF-βRⅡsiRNA后,TGF-β1蛋白表达下降(P<0.05);(3)AngⅡ能促进smad3的磷酸化,转染TGF-βRⅡsiRNA后psmad3蛋白表达下降(P<0.05);(4)AngⅡ促进GMC增殖,转染TGF-βRⅡsiRNA后减轻AngⅡ的增殖作用(P<0.05)。结论 AngⅡ刺激细胞增殖与TGF-β1表达及smad3的磷酸化有关,且依赖TGF-βRⅡ的表达。
文摘Renewable energy (RE) has been attached high attention around the world due to its carbon-free and indigenous production in a sustainable way. China enjoys plenty of renewable energy resources, particularly the wind, solar, hydro- and biomass energy, which could be a sound basis for a large-scale exploitation. This report examines the current status of RE technology and industry, analyzes the challenges of promoting RE in China. In order to pave the way for a long-term development of RE, this paper outlines the basic principles and priorities for individual RE technology. In line with these, the paper puts forward the RE targets and further describes the RE road map by 2020, 2030 and extend to 2050, taking consideration of China’s RE resources, industrial basis and energy demand etc. At last, this paper provides some recommendations to ensure the achievements of the RE targets.
基金Supported by the National Natural Science Foundation of China (Grant Nos. 50525721 and 59837270)
文摘On the foundation of nonlinear robust control and exact generator model, this paper presents a design principle of NR-PSS (Nonlinear Robust Power System Stabilizer) for multi-machine power system, based on which an industrial NR-PSS equipment is developed. For popularizing it, the proposed parameter setting method of NR-PSS is completely the same as the widely used parameter adjustment rule of PSS. By virtue of real time digital simulator (RTDS), large disturbance experiments are carried out to compare the performances between generator excitation system equipped with NR-PSS and PSS in order to verify the correctness of design theory. The results show that compared with classical PSS, the proposed NR-PSS can dramatically improve the generator damping and attenuate the oscillation much faster, enhance the generator damping and raise both the small signal and large disturbance transient stability transmission power limit remarkably. The NR-PSS equipment with independent intellectual property right has been successfully put into operation on a 300 MW generator in Baishan Hydro Plant of Northeast China Grid more than 10 months.
