A JERF36 regulation gene, a selection marker gene (NPT-II), and the foreign genes levansucrase (SacB), Vitreoscilla hemoglobin (vgb), and Binary coleopterus insect resistance (BtCry3A+OC-I) were co-transferred into Po...A JERF36 regulation gene, a selection marker gene (NPT-II), and the foreign genes levansucrase (SacB), Vitreoscilla hemoglobin (vgb), and Binary coleopterus insect resistance (BtCry3A+OC-I) were co-transferred into Populus xeuramericana 'Guariento' using biolistic bombardment; 25 kanamycin resistant plants were obtained. The results of PCR and Southern hybridization showed that the foreign genes had been integrated into the genome of P. xeuramericana 'Guariento' and 5 genes were all transferred into 7 poplar plants. The results of a BtCry3A ELISA experiment indicated that the BtCry3A gene was expressed in the 7 transgenic poplar plants, and these plants grew well on coastal saline land.展开更多
The air-dried density, bending strength (MOR) and compressive strength parallel to grain of different grades of rotten wood were measured with test specimens of 100-130 year-old larch (Larix sp.) collected in Wu Ying ...The air-dried density, bending strength (MOR) and compressive strength parallel to grain of different grades of rotten wood were measured with test specimens of 100-130 year-old larch (Larix sp.) collected in Wu Ying Dian, the Forbidden City. In addition, the MOR of specimens was measured by Resistograph and the correlations were analyzed between resistance value and mechanical properties of wood. The correlations are significant for quantitative evaluation of wood structural condition by Resistograph. The results showed that wood air-dried density, strength properties and resistance values decreased significantly with the increase of decay degree, and there were remarkable differences among various grades of wood. Based on the rotten degree, the attenuation rate is in the following order: MOR> compressive strength parallel to grain > air-dried wood density > resistance value, and the above 4 indexes were 32%, 53%, 73% and 74% of those of intact wood, respectively, when the degree of rotten wood reached grade 3. Significant positive correlation at 0.01 level was showed between the wave trough values and wood air-dried density, MOR, compressive strength parallel to grain. The correlations between the wave peak values and the average value and air-dried wood density, MOR reached 0.01 level, and the correlations between the wave peak values and the average value and compressive strength parallel to grain reached 0.05 level. Based on the correlation between resistance value and physico-mechanical properties of wood, the values could be analyzed quantitatively.展开更多
We addressed the existence and localization of integrin-like in guard cells of Vicia faba by using a probe of polyclonal antibody against the human integrin (αvβ3/β5)-Western blot results showed that three integrin...We addressed the existence and localization of integrin-like in guard cells of Vicia faba by using a probe of polyclonal antibody against the human integrin (αvβ3/β5)-Western blot results showed that three integrins-like of about 47.3, 43.7 and 41.1 ku were detected from the preparation of membrane fragments of purified guard cell protoplasts. Further research with immunofluorescent scanning microscopy indicated that those integrins-like were localized on plasma membrane of guard cells, most nearing the dorsal wall, which is consistent with the reception of signals from epidermal cells to guard cells. Thus our results indicate, for the first time, that integrins-like are present at guard cell plasma membrane of Vicia faba.展开更多
文摘A JERF36 regulation gene, a selection marker gene (NPT-II), and the foreign genes levansucrase (SacB), Vitreoscilla hemoglobin (vgb), and Binary coleopterus insect resistance (BtCry3A+OC-I) were co-transferred into Populus xeuramericana 'Guariento' using biolistic bombardment; 25 kanamycin resistant plants were obtained. The results of PCR and Southern hybridization showed that the foreign genes had been integrated into the genome of P. xeuramericana 'Guariento' and 5 genes were all transferred into 7 poplar plants. The results of a BtCry3A ELISA experiment indicated that the BtCry3A gene was expressed in the 7 transgenic poplar plants, and these plants grew well on coastal saline land.
基金This work was supported by Ministry of Science and Technology Project (No.2004DIB5J187) and State Administration of CultureHeritage Project (No. 2003022)
文摘The air-dried density, bending strength (MOR) and compressive strength parallel to grain of different grades of rotten wood were measured with test specimens of 100-130 year-old larch (Larix sp.) collected in Wu Ying Dian, the Forbidden City. In addition, the MOR of specimens was measured by Resistograph and the correlations were analyzed between resistance value and mechanical properties of wood. The correlations are significant for quantitative evaluation of wood structural condition by Resistograph. The results showed that wood air-dried density, strength properties and resistance values decreased significantly with the increase of decay degree, and there were remarkable differences among various grades of wood. Based on the rotten degree, the attenuation rate is in the following order: MOR> compressive strength parallel to grain > air-dried wood density > resistance value, and the above 4 indexes were 32%, 53%, 73% and 74% of those of intact wood, respectively, when the degree of rotten wood reached grade 3. Significant positive correlation at 0.01 level was showed between the wave trough values and wood air-dried density, MOR, compressive strength parallel to grain. The correlations between the wave peak values and the average value and air-dried wood density, MOR reached 0.01 level, and the correlations between the wave peak values and the average value and compressive strength parallel to grain reached 0.05 level. Based on the correlation between resistance value and physico-mechanical properties of wood, the values could be analyzed quantitatively.
基金This work was supported by the Major State Basic Research Program of China (Grant No. G1999011704) and the National Natural Science Foundation of China (Grant No. 39730050).
文摘We addressed the existence and localization of integrin-like in guard cells of Vicia faba by using a probe of polyclonal antibody against the human integrin (αvβ3/β5)-Western blot results showed that three integrins-like of about 47.3, 43.7 and 41.1 ku were detected from the preparation of membrane fragments of purified guard cell protoplasts. Further research with immunofluorescent scanning microscopy indicated that those integrins-like were localized on plasma membrane of guard cells, most nearing the dorsal wall, which is consistent with the reception of signals from epidermal cells to guard cells. Thus our results indicate, for the first time, that integrins-like are present at guard cell plasma membrane of Vicia faba.
