Rheumatoid arthritis(RA)is a worldwide public health problem.Interventions to delay or prevent the onset of RA have attracted much attention in recent years,and researchers are now exploring various prevention strateg...Rheumatoid arthritis(RA)is a worldwide public health problem.Interventions to delay or prevent the onset of RA have attracted much attention in recent years,and researchers are now exploring various prevention strategies.At present,there is still no unified consensus for RA prevention,but targeting therapeutic windows and implementing interventions for at-risk individuals are extremely important.Due to the limited number of clinical trials on pharmacologic interventions,further studies are needed to explore and establish optimal intervention regimens and effective measures to prevent progression to RA.In this review,we introduce the RA disease process and risk factors,and present research on the use of both Western and Chinese medicine from clinical perspectives regarding RA prevention.Furthermore,we describe several complete and ongoing clinical studies on the use of Chinese herbal formulae for the prevention of RA.展开更多
Nagilactone E(NLE),a natural product with anticancer activities,is isolated from Podocarpus nagi.In this study,we reported that NLE increased programmed death ligand 1(PD-L1)expressions at both protein and mRNA levels...Nagilactone E(NLE),a natural product with anticancer activities,is isolated from Podocarpus nagi.In this study,we reported that NLE increased programmed death ligand 1(PD-L1)expressions at both protein and mRNA levels in human lung cancer cells,and enhanced its localization on the cell membrane.Mechanistically,NLE increased the phosphorylation and expression of cJun,and promoted the localization of c-Jun in the nucleus,while silencing of c-Jun by small interfering RNA(siRNA)reduced NLEinduced PD-L1.Further study showed that NLE activated the c-Jun N-terminal kinases(JNK),the upstream of c-Jun,and its inhibitor SP600125 reversed the NLE-increased PD-L1.Moreover,NLE-induced PD-L1 increased the binding intensity of PD-1 on the cell surface.In summary,NLE upregulates the expression of PD-L1 in lung cancer cells through the activation of JNK-c-Jun axis,which has the potential to combine with the PD-1/PD-L1 antibody therapies in lung cancer.展开更多
基金Supported by the National Natural Science Foundation of China(No.82274421)the Research Project of Guangdong Provincial Hospital of Chinese Medicine(Nos.2023KT14118,YN2023RD01,YN2021RD01,MY2022KF08)+3 种基金the Specific Fund of State Key Laboratory of Dampness Syndrome of Chinese Medicine(Nos.SZ2021ZZ0202,SZ2021ZZ35)the Guangdong Provincial Key Laboratory of Chinese Medicine for Prevention and Treatment of Refractory Chronic Diseases(2018):2018B030322012(No.YN2023MB15)the Science and Technology Planning Project of Guangdong Province(Nos.2023B1212060063,YN2023ZH22)the Project of Administration of Traditional Chinese Medicine of Guangdong Province of China(No.20231112)。
文摘Rheumatoid arthritis(RA)is a worldwide public health problem.Interventions to delay or prevent the onset of RA have attracted much attention in recent years,and researchers are now exploring various prevention strategies.At present,there is still no unified consensus for RA prevention,but targeting therapeutic windows and implementing interventions for at-risk individuals are extremely important.Due to the limited number of clinical trials on pharmacologic interventions,further studies are needed to explore and establish optimal intervention regimens and effective measures to prevent progression to RA.In this review,we introduce the RA disease process and risk factors,and present research on the use of both Western and Chinese medicine from clinical perspectives regarding RA prevention.Furthermore,we describe several complete and ongoing clinical studies on the use of Chinese herbal formulae for the prevention of RA.
基金supported by the Science and Technology Development Fund,Macao SAR(No.176/2017/A3)University of Macao(No.MYRG2018-00165-ICMS/CPG.020-00016-ICMS/MYRG2017-00109-ICMS)。
文摘Nagilactone E(NLE),a natural product with anticancer activities,is isolated from Podocarpus nagi.In this study,we reported that NLE increased programmed death ligand 1(PD-L1)expressions at both protein and mRNA levels in human lung cancer cells,and enhanced its localization on the cell membrane.Mechanistically,NLE increased the phosphorylation and expression of cJun,and promoted the localization of c-Jun in the nucleus,while silencing of c-Jun by small interfering RNA(siRNA)reduced NLEinduced PD-L1.Further study showed that NLE activated the c-Jun N-terminal kinases(JNK),the upstream of c-Jun,and its inhibitor SP600125 reversed the NLE-increased PD-L1.Moreover,NLE-induced PD-L1 increased the binding intensity of PD-1 on the cell surface.In summary,NLE upregulates the expression of PD-L1 in lung cancer cells through the activation of JNK-c-Jun axis,which has the potential to combine with the PD-1/PD-L1 antibody therapies in lung cancer.
