Analgesis and wound healing effect of chitosan and carboxymethyl chitosan on scalded rats were investigated. A II degree scald model was established in rats, which was subsequently treated with chitosan and carboxymet...Analgesis and wound healing effect of chitosan and carboxymethyl chitosan on scalded rats were investigated. A II degree scald model was established in rats, which was subsequently treated with chitosan and carboxymethyl chitosan solution, respectively. The concentration of bradykinin and 5-hydroxytryptophan was detected by assaying enzyme-linked immunosorbent. Healing condition was observed and pathological sections were made to determine the healing effect of chitosan and carboxymethyl chitosan. Results showed that the concentration of bradykinin and 5-hydroxytryptophan peaked at the third hour post-wound in all groups, while the concentration of hydroxyproline peaked at the seventh day post-wound in both chitosan and carboxymethyl chitosan group. The concentration of bradykinin and 5-hydroxytryptophan of carboxymethyl chitosan group was significantly lower than that of control(P < 0.05), while that of chitosan group was similar to that of control(P > 0.05). These findings indicated that carboxymethyl chitosan reduced the concentration of algogenic substances, resulting in analgesia. During the whole recovery process, the hydroxyproline concentration in chitosan and carboxymethyl chitosan group on day 3 and 7 was significantly higher than that of control(P < 0.01); however the significance of such a highness decreased on day 14(P < 0.05). These findings indicated that chitosan and carboxymethyl chitosan accelerated tissue repair. Meanwhile, chitosan performed better in healing than carboxymethyl chitosan in both decrustation and healing time. In conclusion, carboxymethyl chitosan showed significant analgesis and wound-healing promotion effect, but chitosan only showed wound-healing promotion effect.展开更多
目的观察脓毒性休克并急性呼吸窘迫综合征(acute respiratory distress syndrome,ARDS)患者乳酸清除率(lactate clearance rate,LCR)及血清多配体聚糖-1(syndecan-1,SDC-1)的变化,评价其在预测预后中的作用价值。方法选取2021年2月至202...目的观察脓毒性休克并急性呼吸窘迫综合征(acute respiratory distress syndrome,ARDS)患者乳酸清除率(lactate clearance rate,LCR)及血清多配体聚糖-1(syndecan-1,SDC-1)的变化,评价其在预测预后中的作用价值。方法选取2021年2月至2022年4月期间入住郑州大学附属郑州市中心医院呼吸重症监护病房(respiratory intensive care unit,RICU)的脓毒性休克并ARDS患者为研究对象,根据患者28 d生存情况分为生存组和死亡组。收集两组的患者的一般临床资料及相关指标,并进行组间比较。分析各时间点变量间的相关性;筛查影响脓毒性休克并ARDS患者28 d死亡的相关因素,并绘制受试者工作特征曲线(ROC曲线),评估LCR、SDC-1对脓毒性休克并ARDS患者预后的单独及联合预测价值。结果与生存组相比,死亡组入住RICU时的序贯器官衰竭评分(sequential organ failure assessment,SOFA)及急性生理学与慢性健康状况评分(acute physiology and chronic health evaluation,APACHE)Ⅱ、24 h Lac、6 h SDC-1、24 h SDC-1、72 h SDC-1水平显著升高(均P<0.05),6 h LCR、24 h LCR、6 h OI、24 h OI、72 h OI显著降低(均P<0.05)。Spearman相关性分析显示6 h、24 h、72 h的SDC-1与对应时间点的OI均成显著负相关(均P<0.05),6 h与24 h的LCR与对应时间点的OI均成显著正相关(均P<0.05)。多因素Logistic回归分析显示SOFA评分、24 h LCR、24 h SDC-1、72 h SDC-1是脓毒性休克并ARDS患者28 d死亡相关因素(均P<0.05)。各个相关因素的ROC曲线下面积分别为SOFA评分、24 h LCR、24 h SDC-1、72 h SDC-1对预后有预测价值(均P<0.05),24 h LCR联合24 h SDC-1具有最大曲线下面积为0.805(95%CI:0.691~0.920),敏感度为75.0%,特异度为74.4%。结论24 h LCR、24 h SDC-1、72 h SDC-1是影响脓毒性休克并ARDS患者28 d死亡的相关因素;联合检测24 h LCR与24 h SDC-1较单独检测可以提升检验效能。展开更多
目的调查乳腺癌患者睡眠质量状况,并分析相关因素对睡眠质量的影响。方法选取2018年7月至11月就诊的女性乳腺癌患者100例作为病例组,同期行健康筛查的女性200例作为对照组,利用自行设计的一般资料调查表和匹兹堡睡眠质量指数量表(PSQI)...目的调查乳腺癌患者睡眠质量状况,并分析相关因素对睡眠质量的影响。方法选取2018年7月至11月就诊的女性乳腺癌患者100例作为病例组,同期行健康筛查的女性200例作为对照组,利用自行设计的一般资料调查表和匹兹堡睡眠质量指数量表(PSQI)收集研究对象人口学特征和睡眠质量,通过查阅病历系统获取患者的疾病相关信息。采用线性回归分析法分析乳腺癌患者睡眠质量的相关因素。结果病例组患者睡眠质量较对照组差(6.46±3.58 vs 4.99±3.06,t=3.512,P<0.001),根据划界分,病例组67.0%的患者睡眠质量判定为差,高于对照组的44.5%(χ~2=13.522,P<0.001)。乳房切除手术较未手术(B=2.81,Beta=0.38, 95%CI:0.77～4.85,P=0.007)及保乳手术(B=2.92,Beta=0.40,95%CI:1.40～4.45,P<0.001)的患者睡眠质量差。结论乳腺癌患者的睡眠质量较健康女性差,乳房切除手术是患者睡眠质量差的一个重要影响因素。提示临床应重视对乳腺癌术后患者睡眠质量的评估。展开更多
基金supported by National High-Tech R&D Program of China (863 Program, 2014AA093605)
文摘Analgesis and wound healing effect of chitosan and carboxymethyl chitosan on scalded rats were investigated. A II degree scald model was established in rats, which was subsequently treated with chitosan and carboxymethyl chitosan solution, respectively. The concentration of bradykinin and 5-hydroxytryptophan was detected by assaying enzyme-linked immunosorbent. Healing condition was observed and pathological sections were made to determine the healing effect of chitosan and carboxymethyl chitosan. Results showed that the concentration of bradykinin and 5-hydroxytryptophan peaked at the third hour post-wound in all groups, while the concentration of hydroxyproline peaked at the seventh day post-wound in both chitosan and carboxymethyl chitosan group. The concentration of bradykinin and 5-hydroxytryptophan of carboxymethyl chitosan group was significantly lower than that of control(P < 0.05), while that of chitosan group was similar to that of control(P > 0.05). These findings indicated that carboxymethyl chitosan reduced the concentration of algogenic substances, resulting in analgesia. During the whole recovery process, the hydroxyproline concentration in chitosan and carboxymethyl chitosan group on day 3 and 7 was significantly higher than that of control(P < 0.01); however the significance of such a highness decreased on day 14(P < 0.05). These findings indicated that chitosan and carboxymethyl chitosan accelerated tissue repair. Meanwhile, chitosan performed better in healing than carboxymethyl chitosan in both decrustation and healing time. In conclusion, carboxymethyl chitosan showed significant analgesis and wound-healing promotion effect, but chitosan only showed wound-healing promotion effect.
文摘目的观察脓毒性休克并急性呼吸窘迫综合征(acute respiratory distress syndrome,ARDS)患者乳酸清除率(lactate clearance rate,LCR)及血清多配体聚糖-1(syndecan-1,SDC-1)的变化,评价其在预测预后中的作用价值。方法选取2021年2月至2022年4月期间入住郑州大学附属郑州市中心医院呼吸重症监护病房(respiratory intensive care unit,RICU)的脓毒性休克并ARDS患者为研究对象,根据患者28 d生存情况分为生存组和死亡组。收集两组的患者的一般临床资料及相关指标,并进行组间比较。分析各时间点变量间的相关性;筛查影响脓毒性休克并ARDS患者28 d死亡的相关因素,并绘制受试者工作特征曲线(ROC曲线),评估LCR、SDC-1对脓毒性休克并ARDS患者预后的单独及联合预测价值。结果与生存组相比,死亡组入住RICU时的序贯器官衰竭评分(sequential organ failure assessment,SOFA)及急性生理学与慢性健康状况评分(acute physiology and chronic health evaluation,APACHE)Ⅱ、24 h Lac、6 h SDC-1、24 h SDC-1、72 h SDC-1水平显著升高(均P<0.05),6 h LCR、24 h LCR、6 h OI、24 h OI、72 h OI显著降低(均P<0.05)。Spearman相关性分析显示6 h、24 h、72 h的SDC-1与对应时间点的OI均成显著负相关(均P<0.05),6 h与24 h的LCR与对应时间点的OI均成显著正相关(均P<0.05)。多因素Logistic回归分析显示SOFA评分、24 h LCR、24 h SDC-1、72 h SDC-1是脓毒性休克并ARDS患者28 d死亡相关因素(均P<0.05)。各个相关因素的ROC曲线下面积分别为SOFA评分、24 h LCR、24 h SDC-1、72 h SDC-1对预后有预测价值(均P<0.05),24 h LCR联合24 h SDC-1具有最大曲线下面积为0.805(95%CI:0.691~0.920),敏感度为75.0%,特异度为74.4%。结论24 h LCR、24 h SDC-1、72 h SDC-1是影响脓毒性休克并ARDS患者28 d死亡的相关因素;联合检测24 h LCR与24 h SDC-1较单独检测可以提升检验效能。
文摘目的调查乳腺癌患者睡眠质量状况,并分析相关因素对睡眠质量的影响。方法选取2018年7月至11月就诊的女性乳腺癌患者100例作为病例组,同期行健康筛查的女性200例作为对照组,利用自行设计的一般资料调查表和匹兹堡睡眠质量指数量表(PSQI)收集研究对象人口学特征和睡眠质量,通过查阅病历系统获取患者的疾病相关信息。采用线性回归分析法分析乳腺癌患者睡眠质量的相关因素。结果病例组患者睡眠质量较对照组差(6.46±3.58 vs 4.99±3.06,t=3.512,P<0.001),根据划界分,病例组67.0%的患者睡眠质量判定为差,高于对照组的44.5%(χ~2=13.522,P<0.001)。乳房切除手术较未手术(B=2.81,Beta=0.38, 95%CI:0.77～4.85,P=0.007)及保乳手术(B=2.92,Beta=0.40,95%CI:1.40～4.45,P<0.001)的患者睡眠质量差。结论乳腺癌患者的睡眠质量较健康女性差,乳房切除手术是患者睡眠质量差的一个重要影响因素。提示临床应重视对乳腺癌术后患者睡眠质量的评估。
