In this study, a forward cDNA library was constructed by suppression subtractive hybridization using seedling leaves of CN165, a drought-tolerant maize inbred line. In the suppression subtractive hybridization （SSH）...In this study, a forward cDNA library was constructed by suppression subtractive hybridization using seedling leaves of CN165, a drought-tolerant maize inbred line. In the suppression subtractive hybridization （SSH） library, 672 positive clones were picked up randomly. After polymerase chain reaction （PCR） of each clone, all the single clones were sequenced. Totally 598 available sequences were obtained. After cluster analysis of the EST sequences, 80 uniESTs were obtained, among which 57 uniESTs were contigs and 23 uniESTs were singlets. The results of BLASTN showed that all the uniESTs had homologous sequences in the nr database. The BLASTX results indicated that 68 uniESTs had significant protein homology, 8 uniESTs with homology of unknown proteins and putative proteins, and 4 uniESTs without protein homology. Those drought stress-induced genes were involved in many metabolism pathways to regulate plant growth and development under drought stress.展开更多
文摘In this study, a forward cDNA library was constructed by suppression subtractive hybridization using seedling leaves of CN165, a drought-tolerant maize inbred line. In the suppression subtractive hybridization （SSH） library, 672 positive clones were picked up randomly. After polymerase chain reaction （PCR） of each clone, all the single clones were sequenced. Totally 598 available sequences were obtained. After cluster analysis of the EST sequences, 80 uniESTs were obtained, among which 57 uniESTs were contigs and 23 uniESTs were singlets. The results of BLASTN showed that all the uniESTs had homologous sequences in the nr database. The BLASTX results indicated that 68 uniESTs had significant protein homology, 8 uniESTs with homology of unknown proteins and putative proteins, and 4 uniESTs without protein homology. Those drought stress-induced genes were involved in many metabolism pathways to regulate plant growth and development under drought stress.
文摘目的使用三维计算机断层扫描(computed tomography,CT)探讨左回旋支动脉(left circumflex coronary artery,LCX)和二尖瓣环之间的解剖关系,并识别二尖瓣术中LCX损伤的危险因素。方法回顾性分析2017年1月至2020年7月福建医科大学附属漳州市医院收治的122例二尖瓣反流患者术前心脏CT图像,判断冠状动脉优势类型及是否存在二尖瓣环分离,使用经食道超声心动图分析二尖瓣反流病因。检测二尖瓣环上最接近LCX的点(X点),测量LCX到二尖瓣环的最小距离(minimum distance between left circumflex artery and mitral annulus,mDistanceLCX-MA)。应用单因素和多元回归分析影响mDistanceLCX-MA的相关因素。结果中位mDis⁃tanceLCX-MA为4.2(0.9~11.4)mm,X点主要位于后瓣环的外侧。51例(42%)患者出现二尖瓣环分离。与其他优势类型相比,左优势型的mDistanceLCX-MA最短(P<0.01)。与心房功能性二尖瓣反流组相比,黏液样病变二尖瓣脱垂组患者的mDistanceLCX-MA明显缩短(P=0.04)。多元回归分析显示,左优势型和二尖瓣环分离是减少mDis⁃tanceLCX-MA的重要独立因素(P值均为0.01)。相反,右优势型是增加mDistanceLCX-MA的重要独立因素(P=0.01)。结论术前心脏三维CT检查有助于判断LCX和二尖瓣环之间的解剖关系。左优势型和二尖瓣环分离的患者在行二尖瓣手术时存在LCX损伤的高风险。
