期刊文献+
共找到3篇文章
< 1 >
每页显示 20 50 100
海洋细菌Microbacterium esteraromaticum MCDA02产几丁质脱乙酰酶发酵条件优化 被引量:1
1
作者 王敏卜 柴金龙 +6 位作者 武波飞 苏永成 陈丽 焦豫良 黄绪娟 房耀维 刘姝 《中国食品添加剂》 CAS 2019年第11期138-146,共9页
从海洋样品中筛选几丁质脱乙酰酶产生菌,对产酶水平较高菌株进行鉴定,并对发酵条件进行优化。采用对硝基乙酰苯胺为指示剂,通过变色圈法筛选获得几丁质脱乙酰酶的海洋细菌菌株MCDA02。通过形态学特征、生理生化特性和16SrDNA序列分析,... 从海洋样品中筛选几丁质脱乙酰酶产生菌,对产酶水平较高菌株进行鉴定,并对发酵条件进行优化。采用对硝基乙酰苯胺为指示剂,通过变色圈法筛选获得几丁质脱乙酰酶的海洋细菌菌株MCDA02。通过形态学特征、生理生化特性和16SrDNA序列分析,将其鉴定为Microbacterium esteraromaticum。通过单因素实验结合响应面设计对菌株MCDA02产几丁质脱乙酰酶的发酵条件进行优化,获得该菌株的最佳培养基配方为胰蛋白胨1%、木薯淀粉1.1%、ZnSO40.05%。发酵条件为初始pH7.9,30℃、180r/min、发酵72h、接种量1%、装液量20%。在此条件下菌株MCDA02发酵产几丁质脱乙酰酶水平2.01U/mL,比优化前提高了1.41倍。 展开更多
关键词 几丁质脱乙酰酶 海洋细菌 鉴定 发酵条件优化
下载PDF
脱氢枞氧基聚氧乙烯缩水甘油醚接枝羟乙基壳聚糖水凝胶制备及其性能
2
作者 黄旭娟 王婷 +3 位作者 丁正青 杨欣欣 蔡照胜 商士斌 《应用化学》 CAS CSCD 北大核心 2022年第9期1421-1428,共8页
通过脱氢枞醇聚氧乙烯(10)醚(DA(EO)_(10)H)与环氧氯丙烷缩合后,再在NaOH存在下脱氯化氢得到脱氢枞氧基聚氧乙烯(10)缩水甘油醚(DA(EO)_(10)GE),然后通过DA(EO)_(10)GE对羟乙基壳聚糖(HECTS)的接枝制备DA(EO)_(10)GE接枝羟乙基壳聚糖(DA... 通过脱氢枞醇聚氧乙烯(10)醚(DA(EO)_(10)H)与环氧氯丙烷缩合后,再在NaOH存在下脱氯化氢得到脱氢枞氧基聚氧乙烯(10)缩水甘油醚(DA(EO)_(10)GE),然后通过DA(EO)_(10)GE对羟乙基壳聚糖(HECTS)的接枝制备DA(EO)_(10)GE接枝羟乙基壳聚糖(DA(EO)_(10)GE-g-HECTS),最后利用Genipin对DA(EO)_(10)GE-g-HECTS进行交联,得到DA(EO)_(10)GE-g-HECTS/Genipin水凝胶。研究结果表明:DA(EO)_(10)GE对HECTS糖单元摩尔比的增加能提高DA(EO)_(10)GE-g-HECTS的接枝度,并延长其与Genipin交联形成凝胶的时间;增加Genipin的用量可提高接枝产物与Genipin交联形成凝胶的能力;负载于DA(EO)_(10)GE-g-HECTS/Genipin凝胶中的氯霉素在人工肠液中的累积释放率与时间的关系,可以很好地用Boltzmann函数描述,且提高接枝度和降低Genipin用量有利于提高药物的最终累积释放率;低接枝度DA(EO)_(10)GE-g-HECTS经Genipin交联形成的载药凝胶,其药物释放行为符合一级动力学方程的特征。 展开更多
关键词 羟乙基壳聚糖 脱氢枞氧基聚氧乙烯缩水甘油醚 水凝胶 药物释放
下载PDF
Simulation of groundwater level recovery in abandoned mines, Fengfeng coalfield, China 被引量:1
3
作者 MA Luan WANG Guang-cai +3 位作者 SHI Zhe-ming GUO Yu-ying XU Qing-yu huang xu-juan 《Journal of Groundwater Science and Engineering》 2016年第4期344-353,共10页
Abandoned mines are of high potential risk as they could be a large underground storage of pollutants(heavy metals and organic wastes, etc.). Various physical, chemical and biological reactions would take place when g... Abandoned mines are of high potential risk as they could be a large underground storage of pollutants(heavy metals and organic wastes, etc.). Various physical, chemical and biological reactions would take place when groundwater flows into underground spaces, which makes abandoned mine a huge potential hazard to groundwater environment. The recovery of groundwater level is one of the key elements controlling the reactions and causing such hazards. This paper simulated groundwater level recovery processes in the abandoned mines, Fengfeng coalfield by using the computer program FEFLOW. The paper integrated the pipe flow model, "three zones" model and groundwater inrush(discharge) model in the simulation of groundwater in the complex laneway-aquifer system. Groundwater flow in the laneway systems was considered pipe flow and described in Bernoulli equation. The water-bearing medium(coal seam roof) overlying the laneway systems was divided into "three zones" composed of the caving zone, fissure zone and bending zone based on the disruption degrees of previous mining. Groundwater in the Ordovician limestone aquifer(bottom of coal seam) flowing into laneway systems was considered a major inrush/recharge source, and its flow rate was calculated by an inrush(discharge) model which was newly developed in this study and incorporated into FEFLOW. The results showed that it would take approximately 95 days for groundwater in abandoned mines to recover to regional groundwater level elevation, and the total amount of water filling up would be about 1.41195×10~7 m^3, which is consistent with the actual data. The study could be of theoretical and practical significance to mitigate abandoned mines' hazards and improve mine groundwater utilization. 展开更多
关键词 Abandoned mine Laneway system Aquifer media FEFLOW program Groundwater level recovery
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部