Objective: To investigate the effect of Lang-chuang-ding Decoction(狼疮定汤, LCD) on the expression of DNA methylation of CD70 gene promoter in peripheral blood mononuclear cells(PBMCs) of females with systemic lupus ...Objective: To investigate the effect of Lang-chuang-ding Decoction(狼疮定汤, LCD) on the expression of DNA methylation of CD70 gene promoter in peripheral blood mononuclear cells(PBMCs) of females with systemic lupus erythematosus(SLE). Methods: PBMCs isolated from female patients with SLE or healthy donors were cultured and treated with LCD medicated serum or normal serum for 24 or 48 h. The m RNA expressions of CD70 gene in PBMCs were detected by reverse transcription polymerase chain reaction(PCR); the DNA methylation of the CD70 gene promoter region was detected by methylation-specific PCR. Results: After treated with medicated serum for 48 h, the m RNA expression levels of CD70 in PBMCs of SLE patients were significantly higher than those of healthy donors(P<0.05); the DNA methylation levels of CD70 promoter region in PBMCs of SLE patients treated with medicated serum for 48 h were significantly higher than those treated with fetal bovine serum(P<0.01). Conclusion: LCD could inhibit CD70 gene expression in PBMCs of SLE patients by promoting the DNA methylation of CD70 gene promoter.展开更多
基金Supported by the National Natural Science Foundation of China(No.81373633)the Traditional Chinese Medicine Scientific Research Fund of Zhejiang Province,China(No.2016ZA095)
文摘Objective: To investigate the effect of Lang-chuang-ding Decoction(狼疮定汤, LCD) on the expression of DNA methylation of CD70 gene promoter in peripheral blood mononuclear cells(PBMCs) of females with systemic lupus erythematosus(SLE). Methods: PBMCs isolated from female patients with SLE or healthy donors were cultured and treated with LCD medicated serum or normal serum for 24 or 48 h. The m RNA expressions of CD70 gene in PBMCs were detected by reverse transcription polymerase chain reaction(PCR); the DNA methylation of the CD70 gene promoter region was detected by methylation-specific PCR. Results: After treated with medicated serum for 48 h, the m RNA expression levels of CD70 in PBMCs of SLE patients were significantly higher than those of healthy donors(P<0.05); the DNA methylation levels of CD70 promoter region in PBMCs of SLE patients treated with medicated serum for 48 h were significantly higher than those treated with fetal bovine serum(P<0.01). Conclusion: LCD could inhibit CD70 gene expression in PBMCs of SLE patients by promoting the DNA methylation of CD70 gene promoter.
