序列相似家族172成员A(family with sequence similarity 172 member A,FAM172A)已被证实与多种肿瘤的发生和恶性转化有关,但是FAM172A在肝细胞癌(hepatocellular carcinoma,HCC)中的作用尚不完全清楚。本研究分析了肿瘤与癌症基因组图...序列相似家族172成员A(family with sequence similarity 172 member A,FAM172A)已被证实与多种肿瘤的发生和恶性转化有关,但是FAM172A在肝细胞癌(hepatocellular carcinoma,HCC)中的作用尚不完全清楚。本研究分析了肿瘤与癌症基因组图谱数据库中50例正常肝组织和50例肝癌组织信息,结合实时荧光定量PCR和蛋白质免疫印迹结果,发现肝癌组织中FAM172A的表达高于正常肝组织。利用短发夹RNA(short hairpin RNA,shRNA)将肝癌细胞系中FAM172A进行敲低,细胞活力检测和细胞克隆形成实验结果表明,FAM172A的敲低显著抑制了细胞的增殖(P<0.01)。此外糖酵解压力测试和Western印迹结果显示,敲低FAM172A可以有效抑制肝癌细胞的糖酵解能力,糖酵解酶己糖激酶2(hexokinase 2,HK2)、肝内磷酸果糖激酶(phosphofructokinaseliver,PFKL)、乳酸脱氢酶A(lactate dehydrogenase A,LDHA)及其转录因子c-Myc的表达也明显下调(P<0.001)。过表达c-Myc能够解除敲低FAM172A导致的细胞增殖抑制。综上所述,本文揭示了敲低FAM172A抑制肝癌细胞增殖以及发现FAM172A在肝癌中调控有氧糖酵解的新功能。展开更多
Cr_(6+)in K_(2)Cr_(2)O_(7),(NH_(4))_(2)Cr_(2)O_(7),and K_(2)CrO_(4) aqueous solution showed the same new strong band of 794cm^(-1) in surface enhanced Raman scattering(SERS)by using SERS silver substrates.This new fea...Cr_(6+)in K_(2)Cr_(2)O_(7),(NH_(4))_(2)Cr_(2)O_(7),and K_(2)CrO_(4) aqueous solution showed the same new strong band of 794cm^(-1) in surface enhanced Raman scattering(SERS)by using SERS silver substrates.This new feature could be assigned to a surface complex of silver and Cr^(6+),for instance Ag_(2)CrO_(4).The feature of 794cm^(-1) could be a fingerprint band for detecting pollutant Cr^(6+)in water from SERS by using the SERS active substrate of silver.展开更多
Twelve new features from our Raman measurements of C_(70) have been observed by using the silver surface enhanced Raman scattering substrate with very high enhanced factor and highly sensitive Raman set.The results an...Twelve new features from our Raman measurements of C_(70) have been observed by using the silver surface enhanced Raman scattering substrate with very high enhanced factor and highly sensitive Raman set.The results and discussion in this letter would make new knowledge to vibration modes of C_(70).The experimental data of the study confirm the validity of Jishi's calculation with a small error.展开更多
文摘Cr_(6+)in K_(2)Cr_(2)O_(7),(NH_(4))_(2)Cr_(2)O_(7),and K_(2)CrO_(4) aqueous solution showed the same new strong band of 794cm^(-1) in surface enhanced Raman scattering(SERS)by using SERS silver substrates.This new feature could be assigned to a surface complex of silver and Cr^(6+),for instance Ag_(2)CrO_(4).The feature of 794cm^(-1) could be a fingerprint band for detecting pollutant Cr^(6+)in water from SERS by using the SERS active substrate of silver.
文摘Twelve new features from our Raman measurements of C_(70) have been observed by using the silver surface enhanced Raman scattering substrate with very high enhanced factor and highly sensitive Raman set.The results and discussion in this letter would make new knowledge to vibration modes of C_(70).The experimental data of the study confirm the validity of Jishi's calculation with a small error.
