目的探讨数字乳腺体层合成(digital breast tomosynthesis,DBT)鉴别乳腺放射状病变良恶性的应用价值。方法回顾性分析2019年6月—2022年1月华东疗养院67例行数字乳腺X射线摄影(digital mammography,DM)和DBT检查并经病理证实的乳腺放射...目的探讨数字乳腺体层合成(digital breast tomosynthesis,DBT)鉴别乳腺放射状病变良恶性的应用价值。方法回顾性分析2019年6月—2022年1月华东疗养院67例行数字乳腺X射线摄影(digital mammography,DM)和DBT检查并经病理证实的乳腺放射状病变患者的临床和影像资料。以病理结果为金标准,将患者分为良性组和恶性组,比较两组患者的一般基线资料;对比DM和DBT对乳腺放射状病变的诊断效能并分析DBT图像上两组病变中心密度、微钙化、病灶中心的大小、病灶整体的大小及毛刺长度方面的差异。结果67例患者中良性病变38例、恶性病变29例,两组患者年龄与是否绝经差异均有统计学意义(P<0.05)。DM、DBT对乳腺放射状病变检出率及诊断准确率分别为[61.94%(41/67)、100%(67/67)vs.65.85%(27/41)、74.63%(50/67)],两种检查方法的检出率存在显著差异(P<0.001),诊断准确率差异无统计学意义。DM和DBT对恶性放射状病变诊断的灵敏度[65.38%(17/26)vs.82.76%(24/29)]、特异度[66.67%(10/15)vs.68.42%(26/38)]、误诊率[33.34%(5/15)vs.31.58%(12/38)]以及漏诊率[34.62%(9/26)vs.17.24%(5/29)]差异均无统计学意义(P>0.05)。良性组和恶性组在DBT图像上病灶中心大小[(3.30±0.63)mm vs.(4.12±0.65)mm]、病灶整体大小[(24.64±5.28)mm vs.(30.22±7.29)mm]及毛刺长度[(11.32±3.38)mm vs.(13.24±2.23)mm]的差异有统计学意义(P<0.05),病变中心密度及微钙化分布差异无统计学意义。结论DBT对乳腺放射状病变的诊断具有优势。展开更多
2′-5′-Oligoadenylate synthetase like protein(OASL) plays a key role in response to viral infections through selectively activating the OAS/RNase L or OASL/RIG-I signaling pathway.Although classic pathway of OASL is ...2′-5′-Oligoadenylate synthetase like protein(OASL) plays a key role in response to viral infections through selectively activating the OAS/RNase L or OASL/RIG-I signaling pathway.Although classic pathway of OASL is well-known,its regulated genes or co-actors are largely unknown.To study the possible molecular mechanism of duck OASL(dOASL),we performed RNA-sequencing(RNA-seq) and immunoprecipitation and mass spectrometry(IP-MS) at the level of mRNA and protein,respectively.For RNA-seq,we used DF1 cell lines(DF1 dO ASL+/+,DF1 cO ASL–/–,and DF1) with or without the CK/0513 H5 N1 virus(A/chicken/huabei/0513/2007) infection.1 737 differentially expressed genes(DEGs) were identified as candidate target genes regulated by dOASL.Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG) analysis and Weighted Correlation Network Analysis(WGCNA) were performed.We identified one important yellow co-expression module correlated with antiviral immune response.In this module,Ankyrin repeat and FYVE domain containing 1(ANKFY1),harboring a BTB domain similar to the methyl CpG-binding protein 1(MBD1) which bound to OASL in human,was regulated by dOASL.At protein level,133 host proteins were detected.Interestingly,ANKFY1 was one of them binding to dOASL protein.Further phylogenomic and chromosomal syntenic analysis demonstrated MBD1 was absent in birds,while mammals retained.It is suggested that OASL-ANKFY1 interaction might act as a compensatory mechanism to regulate gene expression in birds.Our findings will provide a useful resource for the molecular mechanism research of dOASL.展开更多
H5N1 influenza represents one of the great challenges to public health.Some H5N1 viruses(i.e.,A/goose/Hubei/65/05,GS/65) are weakly pathogenic,while the others(i.e.,A/duck/Hubei/49/05,DK/49) are highly pathogenic to t...H5N1 influenza represents one of the great challenges to public health.Some H5N1 viruses(i.e.,A/goose/Hubei/65/05,GS/65) are weakly pathogenic,while the others(i.e.,A/duck/Hubei/49/05,DK/49) are highly pathogenic to their natural hosts.Here,we performed brain and spleen transcriptomic analyses of control ducks and ones infected by the DK/49 or the GS/65 H5N1 virus.We demonstrated that,compared to the GS/65 virus,the DK/49 virus infection changed more numerous immune genes’ expression and caused continuous increasing of immune pathways(i.e.,RIG-I and MDA5) in ducks.We found that both H5N1 virus strains might escape or subvert host immune response through affecting alternative translation of immune genes,while the DK/49 virus seemed to induce alternative translation of more immune genes than the GS/65 virus.We also identified five co-expressional modules associated with H5N1 virus replication through the weight correlation network analysis(WGCNA).Moreover,we first demonstrated that the duck BCL2 L15 and DCSTAMP in one of these five modules inhibited both the highly pathogenic and weakly pathogenic H5N1 virus replication efficiently.These analyses,in combination with our comprehensive transcriptomic data,provided global view of the molecular architecture for the interaction between host and H5N1 viruses.展开更多
基金the National Natural Science Foundation of China(31772587)the National Key Research and Development Program of China(2016YFD0500202)
文摘2′-5′-Oligoadenylate synthetase like protein(OASL) plays a key role in response to viral infections through selectively activating the OAS/RNase L or OASL/RIG-I signaling pathway.Although classic pathway of OASL is well-known,its regulated genes or co-actors are largely unknown.To study the possible molecular mechanism of duck OASL(dOASL),we performed RNA-sequencing(RNA-seq) and immunoprecipitation and mass spectrometry(IP-MS) at the level of mRNA and protein,respectively.For RNA-seq,we used DF1 cell lines(DF1 dO ASL+/+,DF1 cO ASL–/–,and DF1) with or without the CK/0513 H5 N1 virus(A/chicken/huabei/0513/2007) infection.1 737 differentially expressed genes(DEGs) were identified as candidate target genes regulated by dOASL.Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG) analysis and Weighted Correlation Network Analysis(WGCNA) were performed.We identified one important yellow co-expression module correlated with antiviral immune response.In this module,Ankyrin repeat and FYVE domain containing 1(ANKFY1),harboring a BTB domain similar to the methyl CpG-binding protein 1(MBD1) which bound to OASL in human,was regulated by dOASL.At protein level,133 host proteins were detected.Interestingly,ANKFY1 was one of them binding to dOASL protein.Further phylogenomic and chromosomal syntenic analysis demonstrated MBD1 was absent in birds,while mammals retained.It is suggested that OASL-ANKFY1 interaction might act as a compensatory mechanism to regulate gene expression in birds.Our findings will provide a useful resource for the molecular mechanism research of dOASL.
基金funded by the National Natural Science Foundation of China(31471176)the Fundamental Research Funds for the Central Universities,China(15054034)
文摘H5N1 influenza represents one of the great challenges to public health.Some H5N1 viruses(i.e.,A/goose/Hubei/65/05,GS/65) are weakly pathogenic,while the others(i.e.,A/duck/Hubei/49/05,DK/49) are highly pathogenic to their natural hosts.Here,we performed brain and spleen transcriptomic analyses of control ducks and ones infected by the DK/49 or the GS/65 H5N1 virus.We demonstrated that,compared to the GS/65 virus,the DK/49 virus infection changed more numerous immune genes’ expression and caused continuous increasing of immune pathways(i.e.,RIG-I and MDA5) in ducks.We found that both H5N1 virus strains might escape or subvert host immune response through affecting alternative translation of immune genes,while the DK/49 virus seemed to induce alternative translation of more immune genes than the GS/65 virus.We also identified five co-expressional modules associated with H5N1 virus replication through the weight correlation network analysis(WGCNA).Moreover,we first demonstrated that the duck BCL2 L15 and DCSTAMP in one of these five modules inhibited both the highly pathogenic and weakly pathogenic H5N1 virus replication efficiently.These analyses,in combination with our comprehensive transcriptomic data,provided global view of the molecular architecture for the interaction between host and H5N1 viruses.
