现有的深度学习研究都依赖于网络的自发学习能力,在训练过程中力求避免或尽量减少人为先验知识的设定,导致网络训练过程完全“黑盒”,研究人员很难从语义上进行阐述。针对这种情况,文中提出了一种基于原始LSTM网络的改进——多尺度层级L...现有的深度学习研究都依赖于网络的自发学习能力,在训练过程中力求避免或尽量减少人为先验知识的设定,导致网络训练过程完全“黑盒”,研究人员很难从语义上进行阐述。针对这种情况,文中提出了一种基于原始LSTM网络的改进——多尺度层级LSTM(Multi-Scale Hierarchical Long Short-Term Memory,MSH-LSTM)网络。该网络保留了神经网络的常规实现流程,在网络学习过程中将层级网络结构与人的经验知识有机结合,使网络在人为指引下有目的地训练,不再是完全的“黑盒”,同时对时间序列更好地进行分析预测。为说明MSH-LSTM网络结构的有效性,实验选取了两种时间序列数据(气温、股票),结果表明,相较于ANN网络、LSTM网络及GRU网络,MSH-LSTM网络在保证网络适用性的同时更具分析预测优势。在气温实验中,由于MSH-LSTM与常规LSTM,GRU网络都利用了序列数据的时间因素,因此,它们的效果明显优于ANN;在股票实验中,MSH-LSTM的MAPE误差相对于常规LSTM,GRU,ANN网络分别平均提升了约19.65%,24.35%,46.30%。展开更多
Objective:To study the effects of characteristic Li-medicine Alpinia officinarum Hance on improving insulin resistance(IR),and provide scientific evidence for the adjuvant treatment T2DM.Methods:CCK8 kit was used to d...Objective:To study the effects of characteristic Li-medicine Alpinia officinarum Hance on improving insulin resistance(IR),and provide scientific evidence for the adjuvant treatment T2DM.Methods:CCK8 kit was used to detect the viability of HepG2 cells with 0-200μg/mL Alpinia officinarum Hance extract(AOE)and 0-100μmol/L galangin,and determine the administration concentration.The IR model was established by inducing HepG2 cells with high glucose for 48 hours,and rosiglitazone(ROSI)was used as the positive control drug.Furthermore,we detected intracellular and extracellular glucose contents in IR-HepG2 cells after dosing AOE and galangin by flow cytometry and glucose detection kit,and evaluated the effect of regulating glucose metabolism and IR.Western blot was used to detect the effects of AOE and galangin on the expression of glucose transporter-4(GLUT4)in IR-HepG2 cells.Results:CCK8 kit test result showed that 0-200μg/mL AOE and 0-50μmol/L galangin had no significant effect on HepG2 cell viability.The results of flow cytometry and glucose detection kit showed that the glucose uptake and consumption of the IR model group induced by high glucose was significantly lower than that of the blank group,and thus the IR model was successfully established.Both 50μg/mL AOE and 10,20μmol/L galangin significantly increased the glucose uptake and consumption of IR-HepG2 cells(P<0.05);Western blot experiment showed that AOE and galangin significantly increased GLUT4 level of IR-HepG2 cells(P<0.01).Conclusion:Alpinia officinarum Hance could promote the uptake and consumption of glucose in IR-HepG2 cells by up-regulating the expression of GLUT4 protein,thereby improving IR and enhancing insulin sensitivity.展开更多
文摘现有的深度学习研究都依赖于网络的自发学习能力,在训练过程中力求避免或尽量减少人为先验知识的设定,导致网络训练过程完全“黑盒”,研究人员很难从语义上进行阐述。针对这种情况,文中提出了一种基于原始LSTM网络的改进——多尺度层级LSTM(Multi-Scale Hierarchical Long Short-Term Memory,MSH-LSTM)网络。该网络保留了神经网络的常规实现流程,在网络学习过程中将层级网络结构与人的经验知识有机结合,使网络在人为指引下有目的地训练,不再是完全的“黑盒”,同时对时间序列更好地进行分析预测。为说明MSH-LSTM网络结构的有效性,实验选取了两种时间序列数据(气温、股票),结果表明,相较于ANN网络、LSTM网络及GRU网络,MSH-LSTM网络在保证网络适用性的同时更具分析预测优势。在气温实验中,由于MSH-LSTM与常规LSTM,GRU网络都利用了序列数据的时间因素,因此,它们的效果明显优于ANN;在股票实验中,MSH-LSTM的MAPE误差相对于常规LSTM,GRU,ANN网络分别平均提升了约19.65%,24.35%,46.30%。
基金This study was supported by Natural Science Foundation of Hainan Province(No.820QN273)National Natural Science Foundation of China(No.81860749)。
文摘Objective:To study the effects of characteristic Li-medicine Alpinia officinarum Hance on improving insulin resistance(IR),and provide scientific evidence for the adjuvant treatment T2DM.Methods:CCK8 kit was used to detect the viability of HepG2 cells with 0-200μg/mL Alpinia officinarum Hance extract(AOE)and 0-100μmol/L galangin,and determine the administration concentration.The IR model was established by inducing HepG2 cells with high glucose for 48 hours,and rosiglitazone(ROSI)was used as the positive control drug.Furthermore,we detected intracellular and extracellular glucose contents in IR-HepG2 cells after dosing AOE and galangin by flow cytometry and glucose detection kit,and evaluated the effect of regulating glucose metabolism and IR.Western blot was used to detect the effects of AOE and galangin on the expression of glucose transporter-4(GLUT4)in IR-HepG2 cells.Results:CCK8 kit test result showed that 0-200μg/mL AOE and 0-50μmol/L galangin had no significant effect on HepG2 cell viability.The results of flow cytometry and glucose detection kit showed that the glucose uptake and consumption of the IR model group induced by high glucose was significantly lower than that of the blank group,and thus the IR model was successfully established.Both 50μg/mL AOE and 10,20μmol/L galangin significantly increased the glucose uptake and consumption of IR-HepG2 cells(P<0.05);Western blot experiment showed that AOE and galangin significantly increased GLUT4 level of IR-HepG2 cells(P<0.01).Conclusion:Alpinia officinarum Hance could promote the uptake and consumption of glucose in IR-HepG2 cells by up-regulating the expression of GLUT4 protein,thereby improving IR and enhancing insulin sensitivity.
