目的:运用网络药理学探究虎杖治疗非酒精性脂肪性肝炎(Non-alcoholic steatohepatitis,NASH)的主要活性成分及作用机制。方法:通过中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology,TCMSP)获得虎杖...目的:运用网络药理学探究虎杖治疗非酒精性脂肪性肝炎(Non-alcoholic steatohepatitis,NASH)的主要活性成分及作用机制。方法:通过中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology,TCMSP)获得虎杖主要活性成分,于Pubmed compound数据库下载其结构式并导入Pharmmapper数据库获得成分作用靶点;借助Genecards、OMIM、Disgenet等数据库获得非酒精性脂肪性肝炎的疾病靶点;绘制药物-疾病交集靶点韦恩图,Cytoscape 3.9.1软件构建成分-靶点网络;String数据库获得PPI网络并通过拓扑分析得核心靶点网络,于Metascape数据库对潜在靶点进行GO、KEGG富集分析,微生信在线制图平台绘制气泡图及条形图等。结果:经筛选获得虎杖主要活性成分10个,主要活性成分作用靶点436个,疾病靶点3944个;去重结合得疾病靶点。虎杖抗NASH潜在作用靶点81个,6,8-Dihydroxy-7-methoxyxanthone、luteolin、Physovenine等可能为虎杖作用NASH的主要活性成分;PPI网络拓扑分析得到SRC、RXRA、GRB2、AKT1、RXRB、ESR1、STAT1、JAK2、IGF1、IGF1R等10个核心靶点;GO富集分析主要包含生物过程(Biological process,BP)条目1092个、细胞组分(Cellular component,CC)条目49个、分子功能(Molecular function,MF)条目98个,KEGG分析结果130条,涉及调节细胞对脂质的反应(Cellular response to lipid)、细胞内受体信号通路(Intracellular receptor signaling pathway)等生物过程,通过PI3K/Akt等信号通路发挥作用。结论:虎杖可通过多成分、多靶点、多通路对非酒精性脂肪性肝炎发挥治疗作用,其可能涉及抗炎、抗氧化、抗凋亡、调节代谢、抑制细胞增殖等生物活性。虎杖中的多种活性成分如6,8-Dihydroxy-7-methoxyxanthone、luteolin、Physovenine等可能通过SRC、RXRA、GRB2、AKT1等靶点对信号通路进行调控,涉及多种生物过程,从而发挥抗NASH的作用。展开更多
目的探讨硫氧还蛋白(Trx)、硫氧还蛋白还原酶1(TrxR-1)及硫氧还蛋白互作蛋白(TXNIP)在弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)中的表达及临床意义。方法采用免疫组化法检测60例DLBCL和20例淋巴结反应性增生组织中Trx、...目的探讨硫氧还蛋白(Trx)、硫氧还蛋白还原酶1(TrxR-1)及硫氧还蛋白互作蛋白(TXNIP)在弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)中的表达及临床意义。方法采用免疫组化法检测60例DLBCL和20例淋巴结反应性增生组织中Trx、TrxR-1及TXNIP的表达,并分析三者表达与DLBCL临床病理特征的关系。结果DLBCL中Trx和TrxR-1的阳性率分别为68.33%和76.67%,淋巴结反应性增生组织中Trx和TrxR-1的阳性率分别为25.00%、35.00%,DLBCL中Trx和TrxR-1的阳性率均明显高于淋巴结反应性增生组织,差异有统计学意义(P<0.05);TXNIP在DLBCL中的表达(20.00%,12/60)低于淋巴结反应性增生组织(80.00%,16/20),差异有统计学意义(P<0.05)。DLBCL中Trx、TrxR-1和TXNIP的表达与临床分期、IPI评分有关(P<0.05),与患者性别、年龄、乳酸脱氢酶水平无关(P>0.05)。ROC结果显示,Trx、TrxR-1单项检测曲线下面积(AUC)分别为0.717与0.708,而两者联合检测AUC为0.767,高于单独检测。结论Trx、TrxR-1及TXNIP表达异常可能与DLBCL发生、发展有关,联合检测Trx和TrxR-1表达可为DLBCL的诊断提供参考价值。展开更多
We theoretically investigate the orientation of the cold KRb molecules in a weak electrostatic field assisted by a shaped laser pulse.The results show that the slow turn-on and rapid turn-off laser pulse can enhance t...We theoretically investigate the orientation of the cold KRb molecules in a weak electrostatic field assisted by a shaped laser pulse.The results show that the slow turn-on and rapid turn-off laser pulse can enhance the molecular orientation of KRb significantly,and a high degree of molecular orientation of more than 0.9 can be obtained when the electrostatic field is as low as 3 kV/cm.In addition,the influences of the electrostatic field strength and the parameters of the laser pulse are studied in detail.展开更多
Optically transporting and splitting cold molecules are proposed by spatially pure phase modulating a Gaussian laser beam with a spatial liquid crystal modulator(SLM).Taking the experimentally available cold KRb molec...Optically transporting and splitting cold molecules are proposed by spatially pure phase modulating a Gaussian laser beam with a spatial liquid crystal modulator(SLM).Taking the experimentally available cold KRb molecules(350 nK)as an example,the computations show that the trapping depth is about 460μK and the trap can be split and transported up to 1 cm when a commercially available SLM is employed.展开更多
We propose a scheme to achieve field-free molecular orientation by a nonresonant square laser pulse.Using CO molecules as an example,we show that,differing from the conventional Gaussian pulse,field-free molecular ori...We propose a scheme to achieve field-free molecular orientation by a nonresonant square laser pulse.Using CO molecules as an example,we show that,differing from the conventional Gaussian pulse,field-free molecular orientation excited by square pulses can be realized in both nonadiabatic and adiabatic cases.We also show that the maximum degree of the molecular orientation can be further enhanced by separating one square pulse into two time delayed subpulses and by applying the second subpulse at the beginning of the rotational wave packet rephrasing created by the first subpulse and the optimal intensity ratio between the two subpulses is about I_(1)/I_(2)=1:1.5.展开更多
文摘目的:运用网络药理学探究虎杖治疗非酒精性脂肪性肝炎(Non-alcoholic steatohepatitis,NASH)的主要活性成分及作用机制。方法:通过中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology,TCMSP)获得虎杖主要活性成分,于Pubmed compound数据库下载其结构式并导入Pharmmapper数据库获得成分作用靶点;借助Genecards、OMIM、Disgenet等数据库获得非酒精性脂肪性肝炎的疾病靶点;绘制药物-疾病交集靶点韦恩图,Cytoscape 3.9.1软件构建成分-靶点网络;String数据库获得PPI网络并通过拓扑分析得核心靶点网络,于Metascape数据库对潜在靶点进行GO、KEGG富集分析,微生信在线制图平台绘制气泡图及条形图等。结果:经筛选获得虎杖主要活性成分10个,主要活性成分作用靶点436个,疾病靶点3944个;去重结合得疾病靶点。虎杖抗NASH潜在作用靶点81个,6,8-Dihydroxy-7-methoxyxanthone、luteolin、Physovenine等可能为虎杖作用NASH的主要活性成分;PPI网络拓扑分析得到SRC、RXRA、GRB2、AKT1、RXRB、ESR1、STAT1、JAK2、IGF1、IGF1R等10个核心靶点;GO富集分析主要包含生物过程(Biological process,BP)条目1092个、细胞组分(Cellular component,CC)条目49个、分子功能(Molecular function,MF)条目98个,KEGG分析结果130条,涉及调节细胞对脂质的反应(Cellular response to lipid)、细胞内受体信号通路(Intracellular receptor signaling pathway)等生物过程,通过PI3K/Akt等信号通路发挥作用。结论:虎杖可通过多成分、多靶点、多通路对非酒精性脂肪性肝炎发挥治疗作用,其可能涉及抗炎、抗氧化、抗凋亡、调节代谢、抑制细胞增殖等生物活性。虎杖中的多种活性成分如6,8-Dihydroxy-7-methoxyxanthone、luteolin、Physovenine等可能通过SRC、RXRA、GRB2、AKT1等靶点对信号通路进行调控,涉及多种生物过程,从而发挥抗NASH的作用。
文摘目的探讨硫氧还蛋白(Trx)、硫氧还蛋白还原酶1(TrxR-1)及硫氧还蛋白互作蛋白(TXNIP)在弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)中的表达及临床意义。方法采用免疫组化法检测60例DLBCL和20例淋巴结反应性增生组织中Trx、TrxR-1及TXNIP的表达,并分析三者表达与DLBCL临床病理特征的关系。结果DLBCL中Trx和TrxR-1的阳性率分别为68.33%和76.67%,淋巴结反应性增生组织中Trx和TrxR-1的阳性率分别为25.00%、35.00%,DLBCL中Trx和TrxR-1的阳性率均明显高于淋巴结反应性增生组织,差异有统计学意义(P<0.05);TXNIP在DLBCL中的表达(20.00%,12/60)低于淋巴结反应性增生组织(80.00%,16/20),差异有统计学意义(P<0.05)。DLBCL中Trx、TrxR-1和TXNIP的表达与临床分期、IPI评分有关(P<0.05),与患者性别、年龄、乳酸脱氢酶水平无关(P>0.05)。ROC结果显示,Trx、TrxR-1单项检测曲线下面积(AUC)分别为0.717与0.708,而两者联合检测AUC为0.767,高于单独检测。结论Trx、TrxR-1及TXNIP表达异常可能与DLBCL发生、发展有关,联合检测Trx和TrxR-1表达可为DLBCL的诊断提供参考价值。
基金Supported by the National Natural Science Foundation of China under Grant No 11034002the National Basic Research Program of China under Grant No 2011921602,and Qing Lan Project.
文摘We theoretically investigate the orientation of the cold KRb molecules in a weak electrostatic field assisted by a shaped laser pulse.The results show that the slow turn-on and rapid turn-off laser pulse can enhance the molecular orientation of KRb significantly,and a high degree of molecular orientation of more than 0.9 can be obtained when the electrostatic field is as low as 3 kV/cm.In addition,the influences of the electrostatic field strength and the parameters of the laser pulse are studied in detail.
基金Supported by the National Natural Science Foundation of China under Grant Nos 11034002 and 11274114the National Key Basic Research and Development Program of China under Grant No 2011CB921602the Open Research Fund of the State Key Laboratory of Precision Spectroscopy.
文摘Optically transporting and splitting cold molecules are proposed by spatially pure phase modulating a Gaussian laser beam with a spatial liquid crystal modulator(SLM).Taking the experimentally available cold KRb molecules(350 nK)as an example,the computations show that the trapping depth is about 460μK and the trap can be split and transported up to 1 cm when a commercially available SLM is employed.
基金Supported by the Natural Science Foundation of Jiangsu Province under Grant No BK2008183the Open Research Fund of State Key Laboratory of Precision Spectroscopy,East China Normal University.
文摘We propose a scheme to achieve field-free molecular orientation by a nonresonant square laser pulse.Using CO molecules as an example,we show that,differing from the conventional Gaussian pulse,field-free molecular orientation excited by square pulses can be realized in both nonadiabatic and adiabatic cases.We also show that the maximum degree of the molecular orientation can be further enhanced by separating one square pulse into two time delayed subpulses and by applying the second subpulse at the beginning of the rotational wave packet rephrasing created by the first subpulse and the optimal intensity ratio between the two subpulses is about I_(1)/I_(2)=1:1.5.
