LncRNA PCGEM1 facilitates cervical cancer progression via miR-642a-5p/KIF5B axis

At present,the role of many long non-coding RNAs(lncRNAs)as tumor suppressors in the formation and development of cervical cancer(CC)has been studied.However,lncRNA prostate cancer gene expression marker 1(PCGEM1),whose high expression not only aggravates ovarian cancer but also can induce tumorigenesis and endometrial cancer progression,has not been studied in CC.The objective of this study was to investigate the expression and the underlying role of PCGEM1 in CC.The relative expression of PCGEM1 in CC cells was detected by real-time PCR.After the suppression of PCGEM1 expression by shRNA,the changes in the proliferation,migration,and invasion capacities were detected via CCK-8 assay,EdU assay,and colony formation assay wound healing assay.Transwell assay and the changes in expressions of epithelial-to-mesenchymal transition(EMT)markers were determined by western blot and immunofluorescence.The interplay among PCGEM1,miR-642a-5p,and kinesin family member 5B(KIF5B)was confirmed by bioinformatics analyses and luciferase reporter assay.Results showed that PCGEM1 expressions were up-regulated within CC cells.Cell viabilities,migration,and invasion were remarkably reduced after the suppression of PCGEM1 expression by shRNA in Hela and SiHa cells.N-cadherin was silenced,but E-cadherin expression was elevated by sh-PCGEM1.Moreover,by sponging miR-642a-5p in CC,PCGEM1 was verified as a competitive endogenous RNA(ceRNA)that modulates KIF5B levels.MiR-642a-5p down-regulation partially rescued sh-PCGEM1’s inhibitory effects on cell proliferation,migration,invasion,and EMT process.In conclusion,the PCGEM1/miR-642a-5p/KIF5B signaling axis might be a novel therapeutic target in CC.This study provides a research basis and new direction for targeted therapy of CC.

Exploration on Ideological and Political Construction of Higher Vocational English Course in Southern Xinjiang from the Perspective of "Cultural Confidence"

At present,the lack of Chinese culture in higher vocational English teaching in southern Xinjiang is serious.The existing English curriculum system is not enough to support the goal of cultivating ethnic minority college students cultural confidence,and its curriculum ideological and political construction is still in the initial stage of exploration.The combination of the Chinese national culture and the ethnic culture of Xinjiang and the ideological and political elements organically integrated into cultural confidence have endowed the vocational English education in Xinjiang with the characteristics of the times.In this paper,from the perspective of China s"cultural confidence",ideological and political elements were explored,and the background of ideological and political construction in higher vocational English teaching course in southern Xinjiang was analyzed;it is expounded that the ideological and political construction is an inevitable requirement for the high-quality development of vocational English education in southern Xinjiang,and the content design and implementation path of higher vocational English course was explored to provide new ideas and new ways for the curriculum ideological and political construction of higher vocational English teaching in southern Xinjiang.

Connotation and Construction of "1+X" Certificate System in Higher Vocational Colleges : Taking Hetian Vocational and Technical College as an Example

This paper emphatically sums up the problems existing in the implementation of"1+X"certificate system by taking horticulture major in higher vocational colleges as an example.From the point of view of the improvement of"1+X"certificate system,the reform of talent training program,the improvement of teachers ability and summing up experience,this paper puts forward the updated working mode and ideas to provide strategies for accelerating the development of vocational education in southern Xinjiang.

分析师繁忙影响分析师预测行为吗?

信息竞争性披露导致的分析师繁忙是各国普遍存在的现象，但其对分析师行为的影响尚不明确。本文运用2007-2015年A股上市公司及分析师相关数据，研究了分析师繁忙对分析师预测行为的影响。研究结果发现，信息竞争性披露时，相对于不繁忙的分析师，繁忙分析师发布盈余预测的时效性较差；但其预测信息质量较高且较为大胆；可以用有限关注和信息溢价两个理论对这一现象进行解释。进一步研究发现，明星分析师和工作经验长短不能缓解有限关注带来时效性较差的现象，但在明星分析师和工作经验较长的分析师中，信息溢价带来的质量提高更加显著。本文的研究不但丰富了分析师领域的文献，而且揭示了分析师繁忙对分析师预测行为的影响。