骨肉瘤血清miR-337-3p、miR-484、miR-582表达及临床意义

目的 检测骨肉瘤血清miR-337-3p、miR-484、miR-582表达及其临床意义。方法 收集我院2016年1月至2022年1月期间收治的67例骨肉瘤患者的临床病历资料,另纳入同期60例健康志愿者作为对照组。采用DEGseq方法对血清样本进行全基因组miRNA分析,采用实时荧光定量PCR(qPCR)分析血清和组织样本中miRNA水平。受试者工作特征(ROC)曲线分析miRNA诊断骨肉瘤的效能。Logistic回归模型分析影响骨肉瘤新辅助化疗疗效的因素。Cox风险比例回归模型分析影响骨肉瘤预后的因素。结果 经全基因组miRNA分析,骨肉瘤患者血清中miR-337-3p、miR-484、miR-582、miR-1912、miR-4535的表达显著下调。qPCR检测结果显示,骨肉瘤血清miR-337-3p(0.519±0.249 vs. 1.015±0.420)、miR-484(0.669±0.254 vs. 1.029±0.515)、miR-582(0.516±0.258 vs. 0.996±0.381)均低于健康对照组(P<0.001),同时骨肉瘤组织中miR-337-3p、miR-484、miR-582相对表达量也低于癌旁正常组织(P<0.001)。骨肉瘤血清样本和组织样本中miR-337-3p、miR-484、miR-582表达量分别呈正相关(P<0.001)。ROC曲线显示血清miR-337-3p、miR-484、miR-582对于骨肉瘤有良好的诊断效能,联合曲线下面积(AUC)为0.960(95%CI:0.932~0.989)。Logistic回归分析和Cox风险比例回归模型分析均显示血清miR-337-3p、miR-484、miR-582表达水平是影响骨肉瘤新辅助化疗疗效和总生存的独立影响因素(P<0.05)。结论 血清miR-337-3p、miR-484、miR-582可作为骨肉瘤诊断、预测预后的生物标志物。

从玄府概念演变看中医理论的发展与建构

通过对古今玄府从汗孔门户到气血津液精神之通道的概念诠释,从眼科玄府到多科玄府学说建构过程的剖析,并对其构建及发展过程中产生的中医理论概念演变的特征、中医理论建构的逻辑问题、中医理论建构的实体缺位三个方面的问题加以反思,提出中医理论的发展与构建过程中应注意中医概念的规范化研究、中医理论的逻辑自洽性研究以及理论建构中实体结构的研究。

元宇宙作为媒介:传播的“复得”与“复失”

元宇宙预示了数字革命序列中新一轮的技术创新和网络整合,在昭示着互联网产业大变局之时,也同时宣告着对传播领域的重塑和搅动。虽然"元媒介"的概念先于元宇宙而存在,但它却准确预言了在数字时代新旧媒介相互构成、相互塑造、相互依托的辩证关系,基于这种辩证关系,元宇宙正在加固一种"对传播的传播",即"元传播"的过程。于是,作为元媒介和元传播的元宇宙体系以其内在机制和技术特征为数字时代的传播召回了语境与共识、声音与言语,同时显影出此前为我们所忽视的媒介的自反性与基础设施特性。与此同时,元宇宙也以去身体、去真实、去开放和去隐私,警告着传播在这场变革中所面临的风险和危机。

Landsat time-series land cover mapping with spectral signature extension method

Time-series remote sensing images were previously employed to detect land use and land-cover changes and to analyze related trends. However,land-cover change mapping using time-series remote sensing data,especially medium-resolution imagery,was often constrained by a lack of high-quality training and validation data,especially for historical satellite images. In this study,we tested and evaluated a generalized classifier for time series Landsat Thematic Mapper( TM) imagery based on spectral signature extension. First,a new atmospheric correction procedure and a robust relative normalization method were performed on time-series images to eliminate the radiometric differences between them and to retrieve the surface reflectance. Second,we selected one surface reflectance image from the time series as a source image based on the availability of reliable ground truth data. The spectral signature was then extracted from the training data and the source image. Third,the spectral signature was extended to all the corrected time-series images to build a generalized classifier. This method was tested on a time series consisting of five Landsat TM images of the Tibetan Plateau,and the results showed that the corrected time-series images could be classified effectively from the reference image using the generalized classifier. The overall accuracy achieved was between 88. 35% and 94. 25%,which is comparable with the results obtained using traditional scene-by-scene supervised classification. Results also showed that the performance of the extension method was affected by the difference in acquisition times of the source image and target image.

ftsZ gene and plastid division

Plastid is one of the most important cellular organelles, the normal division process of plastid is essential for the differentiation and development of plant cells. For a long time, morphological observations and genetic analyses to special mutants are the major research fields of plastid division, but the molecular mechanisms underlying plastid division are largely unknown. Because of the endosymbiotic origin, plastid division might have mechanisms in common with those involved in bacterial cell division. It has been proved that several prokaryotic cell division genes also participate in the plastid division. Recently, the mechanisms of prokaryotic cell division have been well documented, which provides a valuable paradigm for understanding the plastid division mechanisms. In plants, the functional analyses of ftsZ, a key gene involved both in bacteria and plastid division, have established the solid foundation for people to understand the plastid division in molecular level. In this paper we will make a review for the research history and progress of plastid division.

Auditors＇ Uphill Battle

In June, Li Jinhua, Auditor General of the National Audit Office of China,submitted a report to the National People's Congress revealing widespread embezzlement of public funds in government departments.

Dwindling Labor Supply

this summer, China encountered an unexpected problem-a dwindling supply of migrant labor. In the southeast coastal area in particular, where manufacturing is heavily concentrated, the shortage of labor has become a quite severe problem.

Venturing Into China

"The idea of China has always exerted a pull on the adventurous type. There is a kind of entrepreneurial Westerner who just can't resist it;

Anti-Trust

In 1993, China promulgated a law against unfair competition, which has served as a basic package of rules for the nation to maintain the normal order of market competition. Though there are clauses and articles in other existing laws that concern policies for competition, no exclusive law against monopolistic competition has yet been hammered out.

Shanda Buys Into Sina

In February, Shanda Interactive Entertainment, China's leading online gaming company, bought a 19.5 percent stake in Sina.com, making it the largest single shareholder in China's top Internet portal.

Measuring Our Greenness

Green GDP has become a buzzword of late. For two decades or more, China's rapid economic growth-and its equally rapid environmental destruction and resource depletion-has astonished the world. But now, China is on the fast track to developing a Green GDP.

OEM No More

In March, China's biggest PC maker Lenovo won the US nod to buy IBM's PC business. It marked yet another milestone in the emergence of Chinese brands, which one day may have as great an impact on Western consumers as Japanese brands do today.

Safety at Work

Although the central government recently announced the effectiveness of its macro-control, there are still some acute inconsistencies and problems within the economy. For one, the supply-demand relationship ot coal, electricity, off and transportation is still very strained.

Mainland and HK Economic Relations Reach New Stage

The Chinese mainland/Hong Kong Closer Economic Partnership Arrangement (CEPA) became effective on Jan. 1, 2004. CEPA covers three areas, namely commodities trade, services trade and trade and investment facilitation. Its basic objective is to phase out tariffs and non-tariff barriers on trade in goods,phase in liberalization of trade in services and promote trade and investment facilitation in an effort to bring about common development by upgrading the economic partnership between the mainland and Hong Kong.

Food Safety Concerns

In China, there is an old saying:food is the first necessity of humans. The main concern of the Chinese used to be the security of the food supply rather than the safety of the food itself. However,after a long time fighting food shortages,China became self-sufficient in food in 1995. At this time, the country began for the first time to regulate food safety. Yet China has still not established a legal systern efficient in ensuring this safety. Many problems are rooted in the administration regime and China's priority of economic development.

New Land Reform Gives Farmers More Control

In coastal provinces where industry and service sectors are better developed, many farmers have found their contracted land to be a burden and have taken on jobs in towns instead.They have quit farming, but continue to pay taxes and fees for their contracted land.

Difficult Future for Automakers in China

Brisk car sales are bringing windfall to automakers. But local players are yet to gain key technology, and foreign makers will soon see their profit margins seriously eroded.