Dielectric barrier discharge （DBD） plasma is one of most promising flow control method for its several advantages. The present work investigates the control authority of nanosecond pulse DBD plasma actuators on a fl...Dielectric barrier discharge （DBD） plasma is one of most promising flow control method for its several advantages. The present work investigates the control authority of nanosecond pulse DBD plasma actuators on a flying wing model＇s aerodynamic characteristics. The aerodynamic forces and moments are studied by means of experiment and numerical simulation. The numerical simulation results are in good agreement with experiment results. Both results indicate that the NS-DBD plasma actuators have negligible effect on aerodynamic forces and moment at the angles of attack smaller than 16-. However, significant changes can be achieved with actuation when the model＇s angle of attack is larger than 16° where the flow separation occurs. The spatial flow field structure results from numerical simulation suggest that the volumetric heat produced by NS-DBD plasma actuator changes the local temperature and density and induces several vortex structures, which strengthen the mixing of the shear layer with the main flow and delay separation or even reattach the separated flow.展开更多
The vortex dynamics of flow over an airfoil controlled by a nanosecond pulse dielectric-barrierdischarge(NS-DBD) actuator is studied at a Reynolds number of 1?×?10~5 through wind tunnel experiments and numerical ...The vortex dynamics of flow over an airfoil controlled by a nanosecond pulse dielectric-barrierdischarge(NS-DBD) actuator is studied at a Reynolds number of 1?×?10~5 through wind tunnel experiments and numerical simulation. The numerical method is validated through comparison of the simulated and measured results regarding the effect of the discharge of an NS-DBD actuator placed on a flat plate. The simulated results show that vorticity is mainly induced by the baroclinic torque after plasma discharge, i.e. the term(■) in the equation of vorticity evolution. Both experimental and simulated results demonstrate that after the discharge of the NS-DBD actuator a series of vortices are developed in the shear layer and pull the high-moment fluid down to the wall, enhancing the mixing of internal and external flows.展开更多
Multiple enzymes perform moonlighting functions distinct from their main roles.UDP-glucose epimerases(UGEs),a subclass of isomerases,catalyze the interconversion of UDP-glucose(UDP-Glc)and UDP-galactose(UDP-Gal).We id...Multiple enzymes perform moonlighting functions distinct from their main roles.UDP-glucose epimerases(UGEs),a subclass of isomerases,catalyze the interconversion of UDP-glucose(UDP-Glc)and UDP-galactose(UDP-Gal).We identified a rice male-sterile mutant,osuge1,with delayed tapetum degradation and abortive pollen.The mutant osuge1 protein lacked UDP-glucose epimerase activity,resulting in higher UDP-Gal content and lower UDP-Glc levels in the osuge1 mutant compared with the wild type.Interestingly,we discovered that OsUGE1 participates in the TIP2/bHLH142–TDR–EAT1/DTD transcriptional regulatory cascade involved in tapetum degradation,in which TIP2 and TDR regulate the expression of OsUGE1 while OsUGE1 regulates the expression of EAT1.In addition,we found that OsUGE1 regulates the expression of its own gene by directly binding to an E-box element in the OsUGE1 promoter.Collectively,our results indicate that OsUGE1 not only functions as a UDP-glucose epimerase but also moonlights as a transcriptional activator to promote tapetum degradation,revealing a novel regulatory mechanism of rice reproductive development.展开更多
Advances in the detection and mapping of messenger RNA(mRNA)N^6-methyladenosine(m 6A)and 5-methylcytosine(m 5C),and DNA N^6-methyldeoxyadenosine(6mA)redefined our understanding of these modifications as additional tie...Advances in the detection and mapping of messenger RNA(mRNA)N^6-methyladenosine(m 6A)and 5-methylcytosine(m 5C),and DNA N^6-methyldeoxyadenosine(6mA)redefined our understanding of these modifications as additional tiers of epigenetic regulation.In plants,the most prevalent internal mRNA modifications,m^6A and m^5C,play crucial and dynamic roles in many processes,including embryo development,stem cell fate determination,trichome branching,leaf morphogenesis,floral transition,stress responses,fruit ripening,and root development.The newly identified and widespread epigenetic marker 6mA DNA methylation is associated with gene expression,plant development,and stress responses.Here,we review the latest research progress on mRNA and DNA epigenetic modifications,including the detection,dynamics,distribution,functions,regulatory proteins,and evolution,with a focus on m^6A,m^5C,and 6mA.We also provide some perspectives on future research of the newly identified and unknown epigenetic modifications of mRNA and DNA in plants.展开更多
基金supported by Funding of Jiangsu Innovation Program for Graduate Education(No. KYLX16_0310)the Fundamental Research Funds for the Central Universities (No. NP2016406)+1 种基金supported by Graduate Innovation Center in NUAA (No. kfjj20170117)China Postdoctoral Science Foundation (No. 2017M610325)
文摘Dielectric barrier discharge （DBD） plasma is one of most promising flow control method for its several advantages. The present work investigates the control authority of nanosecond pulse DBD plasma actuators on a flying wing model＇s aerodynamic characteristics. The aerodynamic forces and moments are studied by means of experiment and numerical simulation. The numerical simulation results are in good agreement with experiment results. Both results indicate that the NS-DBD plasma actuators have negligible effect on aerodynamic forces and moment at the angles of attack smaller than 16-. However, significant changes can be achieved with actuation when the model＇s angle of attack is larger than 16° where the flow separation occurs. The spatial flow field structure results from numerical simulation suggest that the volumetric heat produced by NS-DBD plasma actuator changes the local temperature and density and induces several vortex structures, which strengthen the mixing of the shear layer with the main flow and delay separation or even reattach the separated flow.
基金supported by National Natural Science Foundation of China (No. 51806181)the Open Research Subject of the Key Laboratory (Research Base) of Fluid and Power Machinery (Xihua University), Ministry of Education (szjj2017-086)+3 种基金the Key Scientific Research Fund of Xihua University (No. Z17111)the financial support of the Sichuan Provincial Department of Education (No. 18ZA0458)the Key Laboratory of Aeroengine Pneumatic and Thermal Defense Science and Technology (No. 6142702180101)the ‘Young Scholars’ Program of Xihua University
文摘The vortex dynamics of flow over an airfoil controlled by a nanosecond pulse dielectric-barrierdischarge(NS-DBD) actuator is studied at a Reynolds number of 1?×?10~5 through wind tunnel experiments and numerical simulation. The numerical method is validated through comparison of the simulated and measured results regarding the effect of the discharge of an NS-DBD actuator placed on a flat plate. The simulated results show that vorticity is mainly induced by the baroclinic torque after plasma discharge, i.e. the term(■) in the equation of vorticity evolution. Both experimental and simulated results demonstrate that after the discharge of the NS-DBD actuator a series of vortices are developed in the shear layer and pull the high-moment fluid down to the wall, enhancing the mixing of internal and external flows.
基金The Science Fund for Creative Research Groups of the Natural Science Foundation of Chongqing,China(cstc2021jcyi-cxttx0004)the Chongqing Outstanding Scientists Project(cstc2022ycjh-bgzxm0073)the National Natural Science Foundation of China(32072028,31730063).
文摘Multiple enzymes perform moonlighting functions distinct from their main roles.UDP-glucose epimerases(UGEs),a subclass of isomerases,catalyze the interconversion of UDP-glucose(UDP-Glc)and UDP-galactose(UDP-Gal).We identified a rice male-sterile mutant,osuge1,with delayed tapetum degradation and abortive pollen.The mutant osuge1 protein lacked UDP-glucose epimerase activity,resulting in higher UDP-Gal content and lower UDP-Glc levels in the osuge1 mutant compared with the wild type.Interestingly,we discovered that OsUGE1 participates in the TIP2/bHLH142–TDR–EAT1/DTD transcriptional regulatory cascade involved in tapetum degradation,in which TIP2 and TDR regulate the expression of OsUGE1 while OsUGE1 regulates the expression of EAT1.In addition,we found that OsUGE1 regulates the expression of its own gene by directly binding to an E-box element in the OsUGE1 promoter.Collectively,our results indicate that OsUGE1 not only functions as a UDP-glucose epimerase but also moonlights as a transcriptional activator to promote tapetum degradation,revealing a novel regulatory mechanism of rice reproductive development.
基金This work was supported by funding from the National Transgenic Major Program of China(2019ZX08010-002)to X.G.the National Natural Sci ence Foundation of China(31871606,31671670)toX.G.,and the Recruit ment Program of Global Youth Expert of China to X.G.
文摘Advances in the detection and mapping of messenger RNA(mRNA)N^6-methyladenosine(m 6A)and 5-methylcytosine(m 5C),and DNA N^6-methyldeoxyadenosine(6mA)redefined our understanding of these modifications as additional tiers of epigenetic regulation.In plants,the most prevalent internal mRNA modifications,m^6A and m^5C,play crucial and dynamic roles in many processes,including embryo development,stem cell fate determination,trichome branching,leaf morphogenesis,floral transition,stress responses,fruit ripening,and root development.The newly identified and widespread epigenetic marker 6mA DNA methylation is associated with gene expression,plant development,and stress responses.Here,we review the latest research progress on mRNA and DNA epigenetic modifications,including the detection,dynamics,distribution,functions,regulatory proteins,and evolution,with a focus on m^6A,m^5C,and 6mA.We also provide some perspectives on future research of the newly identified and unknown epigenetic modifications of mRNA and DNA in plants.
