The unmanned aerial vehicle(UAV)self-organizing network is composed of multiple UAVs with autonomous capabilities according to a certain structure and scale,which can quickly and accurately complete complex tasks such...The unmanned aerial vehicle(UAV)self-organizing network is composed of multiple UAVs with autonomous capabilities according to a certain structure and scale,which can quickly and accurately complete complex tasks such as path planning,situational awareness,and information transmission.Due to the openness of the network,the UAV cluster is more vulnerable to passive eavesdropping,active interference,and other attacks,which makes the system face serious security threats.This paper proposes a Blockchain-Based Data Acquisition(BDA)scheme with privacy protection to address the data privacy and identity authentication problems in the UAV-assisted data acquisition scenario.Each UAV cluster has an aggregate unmanned aerial vehicle(AGV)that can batch-verify the acquisition reports within its administrative domain.After successful verification,AGV adds its signcrypted ciphertext to the aggregation and uploads it to the blockchain for storage.There are two chains in the blockchain that store the public key information of registered entities and the aggregated reports,respectively.The security analysis shows that theBDAconstruction can protect the privacy and authenticity of acquisition data,and effectively resist a malicious key generation center and the public-key substitution attack.It also provides unforgeability to acquisition reports under the Elliptic Curve Discrete Logarithm Problem(ECDLP)assumption.The performance analysis demonstrates that compared with other schemes,the proposed BDA construction has lower computational complexity and is more suitable for the UAV cluster network with limited computing power and storage capacity.展开更多
Fertilizers are widely used to produce more food, inevitably altering the diversity and composition of soil organisms. The role of soil biodiversity in controlling multiple ecosystem services remains unclear, especial...Fertilizers are widely used to produce more food, inevitably altering the diversity and composition of soil organisms. The role of soil biodiversity in controlling multiple ecosystem services remains unclear, especially after decades of fertilization. Here, we assess the contribution of the soil functionalities of carbon(C), nitrogen(N), and phosphorus(P) cycling to crop production and explore how soil organisms control these functionalities in a 33-year field fertilization experiment. The long-term application of green manure or cow manure produced wheat yields equivalent to those obtained with chemical N, with the former providing higher soil functions and allowing the functionality of N cycling(especially soil N mineralization and biological N fixation) to control wheat production. The keystone phylotypes within the global network rather than the overall microbial community dominated the soil multifunctionality and functionality of C,N, and P cycling across the soil profile(0–100 cm). We further confirmed that these keystone phylotypes consisted of many metabolic pathways of nutrient cycling and essential microbes involved in organic C mineralization, N_(2)O release, and biological N fixation. The chemical N, green manure, and cow manure resulted in the highest abundances of amoB, nifH, and GH48 genes and Nitrosomonadaceae,Azospirillaceae, and Sphingomonadaceae within the keystone phylotypes, and these microbes were significantly and positively correlated with N_(2)O release, N fixation, and organic C mineralization, respectively. Moreover, our results demonstrated that organic fertilization increased the effects of the network size and keystone phylotypes on the subsoil functions by facilitating the migration of soil microorganisms across the soil profiles and green manure with the highest migration rates. This study highlights the importance of the functionality of N cycling in controlling crop production and keystone phylotypes in regulating soil functions, and provides selectable fertilization strategies for maintaining crop production and soil functions across soil profiles in agricultural ecosystems.展开更多
The healing of critical-sized bone defects(CSD)remains a challenge in orthopedic medicine.In recent years,scaffolds with sophisticated microstructures fabricated by the emerging three-dimensional(3D)printing technolog...The healing of critical-sized bone defects(CSD)remains a challenge in orthopedic medicine.In recent years,scaffolds with sophisticated microstructures fabricated by the emerging three-dimensional(3D)printing technology have lighted up the treatment of the CSD due to the elaborate microenvironments and support they may build.Here,we established a magnesium oxide-reinforced 3D-printed biocompos-ite scaffold to investigate the effect of magnesium-enriched 3D microenvironment on CSD repairing.The composite was prepared using a biodegradable polymer matrix,polycaprolactone(PCL),and the disper-sion phase,magnesium oxide(MgO).With the appropriate surface treatment by saline coupling agent,the MgO dispersed homogeneously in the polymer matrix,leading to enhanced mechanical performance and steady release of magnesium ion(Mg^(2+))for superior cytocompatibility,higher cell viability,advanced osteogenic differentiation,and cell mineralization capabilities in comparison with the pure PCL.The in-vivo femoral implantation and critical-sized cranial bone defect studies demonstrated the importance of the 3D magnesium microenvironment,as a scaffold that released appropriate Mg^(2+) exhibited remarkably increased bone volume,enhanced angiogenesis,and almost recovered CSD after 8-week implantation.Overall,this study suggests that the magnesium-enriched 3D scaffold is a potential candidate for the treatment of CSD in a cell-free therapeutic approach.展开更多
基金supported in part by the National Key R&D Program of China under Project 2020YFB1006004the Guangxi Natural Science Foundation under Grants 2019GXNSFFA245015 and 2019GXNSFGA245004+2 种基金the National Natural Science Foundation of China under Projects 62162017,61862012,61962012,and 62172119the Major Key Project of PCL under Grants PCL2021A09,PCL2021A02 and PCL2022A03the Innovation Project of Guangxi Graduate Education YCSW2021175.
文摘The unmanned aerial vehicle(UAV)self-organizing network is composed of multiple UAVs with autonomous capabilities according to a certain structure and scale,which can quickly and accurately complete complex tasks such as path planning,situational awareness,and information transmission.Due to the openness of the network,the UAV cluster is more vulnerable to passive eavesdropping,active interference,and other attacks,which makes the system face serious security threats.This paper proposes a Blockchain-Based Data Acquisition(BDA)scheme with privacy protection to address the data privacy and identity authentication problems in the UAV-assisted data acquisition scenario.Each UAV cluster has an aggregate unmanned aerial vehicle(AGV)that can batch-verify the acquisition reports within its administrative domain.After successful verification,AGV adds its signcrypted ciphertext to the aggregation and uploads it to the blockchain for storage.There are two chains in the blockchain that store the public key information of registered entities and the aggregated reports,respectively.The security analysis shows that theBDAconstruction can protect the privacy and authenticity of acquisition data,and effectively resist a malicious key generation center and the public-key substitution attack.It also provides unforgeability to acquisition reports under the Elliptic Curve Discrete Logarithm Problem(ECDLP)assumption.The performance analysis demonstrates that compared with other schemes,the proposed BDA construction has lower computational complexity and is more suitable for the UAV cluster network with limited computing power and storage capacity.
基金supported by the National Key Research and Development Program of China(2021YFD1700200)the earmarked fund for CARS-Green manure(CARS-22)the Agricultural Science and Technology Innovation Program of CAAS。
文摘Fertilizers are widely used to produce more food, inevitably altering the diversity and composition of soil organisms. The role of soil biodiversity in controlling multiple ecosystem services remains unclear, especially after decades of fertilization. Here, we assess the contribution of the soil functionalities of carbon(C), nitrogen(N), and phosphorus(P) cycling to crop production and explore how soil organisms control these functionalities in a 33-year field fertilization experiment. The long-term application of green manure or cow manure produced wheat yields equivalent to those obtained with chemical N, with the former providing higher soil functions and allowing the functionality of N cycling(especially soil N mineralization and biological N fixation) to control wheat production. The keystone phylotypes within the global network rather than the overall microbial community dominated the soil multifunctionality and functionality of C,N, and P cycling across the soil profile(0–100 cm). We further confirmed that these keystone phylotypes consisted of many metabolic pathways of nutrient cycling and essential microbes involved in organic C mineralization, N_(2)O release, and biological N fixation. The chemical N, green manure, and cow manure resulted in the highest abundances of amoB, nifH, and GH48 genes and Nitrosomonadaceae,Azospirillaceae, and Sphingomonadaceae within the keystone phylotypes, and these microbes were significantly and positively correlated with N_(2)O release, N fixation, and organic C mineralization, respectively. Moreover, our results demonstrated that organic fertilization increased the effects of the network size and keystone phylotypes on the subsoil functions by facilitating the migration of soil microorganisms across the soil profiles and green manure with the highest migration rates. This study highlights the importance of the functionality of N cycling in controlling crop production and keystone phylotypes in regulating soil functions, and provides selectable fertilization strategies for maintaining crop production and soil functions across soil profiles in agricultural ecosystems.
基金The authors would like to thank Li LI and H.Z.Xie for the technical support.This work was financially supported by the National Natural Science Foundation of China(Nos.82002303 and 81702171)the Guangdong Basic and Applied Basic Research Foundation(Nos.2022A1515011536,2021A1515220093,2021A1515220086,2019A1515111156,and 2022A1515011815)+7 种基金the Scientific Research Foundation of Peking University Shenzhen hospital(No.KYQD2021064)the Health and Medical Research Fund(No.19180712)the Shenzhen Double Chain Project for Innovation and Development Industry supported by the Bureau of Industry and Information Technology of Shenzhen(No.201806081018272960)the Shenzhen Science and Technology Innovation Committee Projects(Nos.JCYJ20190809182213535 and JSGG20180507183242702)the program from Shanghai Municipal Health Commission(No.201740165)the National Key R&D Program of China(No.2018YFC1105100)the Hong Kong Innovation Technology Fund(Nos.ITS/287/17 and ITS/405/18)the Hong Kong Research Grant Council General Research Fund(No.17214516).
文摘The healing of critical-sized bone defects(CSD)remains a challenge in orthopedic medicine.In recent years,scaffolds with sophisticated microstructures fabricated by the emerging three-dimensional(3D)printing technology have lighted up the treatment of the CSD due to the elaborate microenvironments and support they may build.Here,we established a magnesium oxide-reinforced 3D-printed biocompos-ite scaffold to investigate the effect of magnesium-enriched 3D microenvironment on CSD repairing.The composite was prepared using a biodegradable polymer matrix,polycaprolactone(PCL),and the disper-sion phase,magnesium oxide(MgO).With the appropriate surface treatment by saline coupling agent,the MgO dispersed homogeneously in the polymer matrix,leading to enhanced mechanical performance and steady release of magnesium ion(Mg^(2+))for superior cytocompatibility,higher cell viability,advanced osteogenic differentiation,and cell mineralization capabilities in comparison with the pure PCL.The in-vivo femoral implantation and critical-sized cranial bone defect studies demonstrated the importance of the 3D magnesium microenvironment,as a scaffold that released appropriate Mg^(2+) exhibited remarkably increased bone volume,enhanced angiogenesis,and almost recovered CSD after 8-week implantation.Overall,this study suggests that the magnesium-enriched 3D scaffold is a potential candidate for the treatment of CSD in a cell-free therapeutic approach.