Management of traumatic peripheral artery pseudoaneurysm:A 10-year experience at a single center

Purpose: This study aimed to report our 10-year experience with the management of iatrogenic(penetrating trauma) and traumatic(blunt or penetrating trauma) peripheral artery pseudoaneurysms, based on data from a tertiary referral center.Methods: From January 2012 to December 2021, the medical records of consecutive patients with iatrogenic and traumatic peripheral artery pseudoaneurysms were retrospectively reviewed. Patient demographics, clinical features, imaging data, treatment details, and follow-up results were analyzed.Results: Sixty-one consecutive patients were included in this study;48(79%) were men and 13(21%) women,with a mean age of 49.4 ± 13.4 years(range 24–73 years). There were 42 patients(69%) who underwent open surgery, 18(29%) undergoing endovascular embolization or stent implantation, and one(2%) undergoing ultrasound-guided thrombin injection. All patients successfully underwent open or interventional treatment. The median follow-up was 46.8 months(2.5–117.9 months), and the overall reintervention rate was 10%. Of these,one(5%) patient in the interventional treatment group and five(12%) patients in the open surgery group underwent reintervention. The overall complication rate was 8%, with complications occurring only in the open surgery group. No deaths occurred in the peri-operative period. No late complications, such as thrombosis or pseudoaneurysm recurrence, were observed.Conclusion: Peripheral artery pseudoaneurysms arising from iatrogenic or traumatic causes can be effectively treated by both open surgery and interventional procedures in selected patients with acceptable mid-and long-term outcomes.

One-step laparoscopic pancreatic necrosectomy verse surgical step-up approach for infected pancreatic necrosis:a case-control study

BACKGROUND:The surgical step-up approach often requires multiple debridements and might not be suitable for infected pancreatic necrosis(IPN)patients with various abscesses or no safe route for percutaneous catheter drainage(PCD).This case-control study aimed to investigate the safety and effectiveness of one-step laparoscopic pancreatic necrosectomy(LPN)in treating IPN.METHODS:This case-control study included IPN patients undergoing one-step LPN or surgical step-up in our center from January 2015 to December 2020.The short-term and long-term complications after surgery,length of hospital stay,and postoperative ICU stays in both groups were analyzed.Univariate and multivariate logistic regression analyses were performed to explore the risk factors of major complications or death.RESULTS:A total of 53 IPN patients underwent one-step LPN and 37 IPN patients underwent surgical step-up approach in this study.There was no significant difference in the incidence of death,major complications,new-onset diabetes,or new-onset pancreatic exocrine insufficiency between the two groups.However,the length of hospital stay in the one-step LPN group was significantly shorter than that in the surgical step-up group.Univariate regression analysis showed that the surgical approach(one-step/step-up)was not the risk factor for major complications or death.Multivariate logistic regression analysis indicated that computed tomography(CT)severity index,American Society of Anesthesiologists(ASA)class IV,and white blood cell(WBC)were the significant risk factors for major complications or death.CONCLUSION:One-step LPN is as safe and effective as the surgical step-up approach for treating IPN patients,and reduces total hospital stay.

Global analysis of lysine succinylation in patchouli plant leaves

Lysine succinylation is a novel,naturally occurring posttranslational modification(PTM)in living organisms.Global lysine succinylation identification has been performed at the proteomic level in various species;however,the study of lysine succinylation in plant species is relatively limited.Patchouli plant(P.cablin(Blanco)Benth.,Lamiaceae)is a globally important industrial plant and medicinal herb.In the present study,lysine succinylome analysis was carried out in patchouli plants to determine the potential regulatory role of lysine succinylation in patchouli growth,development,and physiology.The global succinylation sites and proteins in patchouli plants were screened with an immunoprecipitation affinity enrichment technique and advanced mass spectrometry-based proteomics.Several bioinformatic analyses,such as function classification and enrichment,subcellular location predication,metabolic pathway enrichment and protein−protein interaction networking,were conducted to characterize the functions of the identified sites and proteins.In total,1097 succinylation sites in 493 proteins were detected in patchouli plants,among which 466 succinylation sites in 241 proteins were repeatedly identified within three independent experiments.The functional characterization of these proteins indicated that the tricarboxylic acid(TCA)cycle,oxidative phosphorylation,photosynthesis processes,and amino acid biosynthesis may be regulated by lysine succinylation.In addition,these succinylated proteins showed a wide subcellular location distribution,although the chloroplast and cytoplasm were the top two preferred cellular components.Our study suggested the important role of lysine succinylation in patchouli plant physiology and biology and could serve as a useful reference for succinylation studies in other medicinal plants.

Heavy Metals Contamination in Greenhouse Soils and Vegetables in Guanzhong, China

This study used a flame atomic absorption spectrometer (FAAS) and atomic fluorescence spec-trophotometer (AFS) to detect the concentrations of chromium (Cr), cadmium (Cd), lead (Pb), hy-drargyrum (Hg) and arsenic (As) in soils and three genotypes of vegetables in greenhouse, as well as analyzed the physical and chemical properties of soils, including soil pH, soil organic matter (OM), basic nutrients, electrical conductivity (EC) and cation exchange capacity (CEC) in Guan- zhong areas, Shaanxi province, China. The results showed that comparing to subsoil, the sampled topsoil is enriched in Cr, Cd, Pb, As and Hg. Cd (0.83 - 3.17 mg·kg-1) and Hg (0.40 - 1.44 mg·kg-1) are exceeding the limited value stated in “the 2006 Greenhouse Vegetable Producing Environmental Quality Evaluation Standards” of 0.40 mg·kg-1 and 0.35 mg·kg-1 respectively. However, Nanzhuang greenhouse soil is within the limits. The heavy metal pollution index (HPI) of soil in Sanyuan (8.10) is the highest and in Dongzhang (4.23) is the lowest. The contents of Pb (0.201 - 0.376 mg·kg-1) were exceeding the limited value (0.20 mg·kg-1) in vegetables species, and Cd (0.0363 - 0.0572 mg·kg-1) in some place were also exceeding the limited value (0.05 mg·kg-1). Greenhouse soils were becoming acidified year after year;the ratios of N, P and K in soil were seriously imbalanced. According to the impacting factors, OM, pH, available P, EC and CEC have obviously effected the accumulation of Cr and Hg. However, there was not enough evidence for the effects of available nitrogen and available potassium.

光致驱动聚醚的主/侧链结构设计及光致形变性能调控

报道了一种通过开环聚合与点击化学侧基修饰制备的后功能化光敏性聚醚材料.以烯丙基缩水甘油醚(AGE)为前驱体,通过新戊酸铯催化可控阴离子开环聚合(ROP)获得聚烯丙基缩水甘油醚(PAGE),并通过巯基-烯(Thiol-ene)点击化学和酯化两步反应将光敏基团肉桂酰氯引入到PAGE侧链上,合成了具有光敏特性的聚醚.PAGE的主链长度与肉桂酰氯侧基接枝率可分别通过调控单体/引发剂比例与反应时间灵活调控.将所得侧链修饰后的PAGE预聚物进行光固化成型,获得的光敏性聚酯薄膜具有较低的玻璃化转变温度(T_(g)),同时可在365和254 nm波长紫外光照下发生可逆[2+2]环加成反应,展现出快速可逆的变形特征.本工作通过开环聚合与点击化学反应设计优化了聚醚主侧链结构,为拓宽光功能性聚醚的使用温度窗口、提升其光致变形灵敏度提供了思路,有望使所得材料进一步在药物可控释放、柔性智能机器人等领域得到更加广泛的应用.

Structural basis of INTAC-regulated transcription

DearEditor,Eukaryotic transcription by RNA polymerase I(Pol I)is a strictly regulated process that involves the interplay of numerous factors.Promoter-proximal pausing is a regulatory mechanism that connects transcription initiation and productive elongation in metazoans(Core and Adelman,2019).Pol II forms a paused elongation complex(PEC)through binding of two transcriptional regulation factors DSIF and NELF(Vos et al.,2018).Following the duration of pausing,Pol II either proceeds into productive elongation or undergoes promoter-proximal premature transcription termination(PTT)(Kamieniarz-Gdula and Proudfoot,2019),which plays a decisive role in determining transcriptional outputs.

KLF4 Inhibits the Activation of Human Hepatic Stellate Cell In Vitro

Objective Hepatic stellate cells(HSCs)play a crucial role in liver fibrosis.Early-stage liver fibrosis is reversible and intimately associated with the state of HSCs.Kruppel-like factor 4(KLF4)plays a pivotal role in a wide array of physiological and pathological processes.This study aimed to investigate the effect of KLF4 on the proliferation,apoptosis and phenotype of quiescent HSCs Methods We designed a KLF4 lentiviral vector and a KLF4 siRNA lentiviral vector,to upregulate and silence KLF4 expression in human HSC LX-2 cells via transfection.Cell proliferation was assessed using the CCK-8 assay.Flow cytometry was used to detect the cell cycle distribution and apoptosis rate.Western blotting was used to determine the levels of some quiescence and activation markers of HSCs Results Overexpression of KLF4 significantly increased the levels of E-cadherin and ZO-1,which are quiescent HSC markers,while significantly decreased the levels of N-cadherin and a-SMA,known activated HSC markers.In contrast,cell proliferation and apoptosis rates were elevated in LX-2 cells in which KLF4 expression was silenced Conclusion KLF4 inhibits the proliferation and activation of human LX-2 HSCs.It might be a key regulatory protein in the maintenance of HSC quiescence and may serve as a target for the inhibition of hepatic fibrosis.

湖南省疾控机构在职工作人员吸烟行为现状及影响因素分析

目的了解湖南省疾控机构在职工作人员吸烟行为现状及影响因素,为控烟工作决策提供科学依据。方法采用分层整群抽样方法抽取湖南省省、市、县三级疾病预防控制中心,对抽中的疾控中心及其在职工作人员分别进行问卷调查,并用单、多因素logistic回归分析筛选与现在是否吸烟有关的因素。结果湖南省疾控机构在职工作人员吸烟率为26.8%,现在吸烟率为21.9%,戒烟率为4.9%;现在吸烟者日平均吸烟量为(15.8±9.5)支,现在每日吸烟者的日平均吸烟量为(18.1±8.6)支;吸烟会导致疾病认识全对的比例为26.2%,同时知晓二手烟会导致三种疾病的比例为46.3%,对低焦油卷烟危害正确认知的比例为33.4%;采用无烟医疗卫生机构标准对各被调查单位进行调查评分,平均得分66.5分,最高为85分(满分),最低为37分;多因素logistic回归分析表明,性别(OR=156.898,OR95%CI:64.510~381.595)、年龄(OR=4.581,OR95%CI:2.177~9.641)、文化程度(OR=0.248,OR95%CI:0.074~0.833)、“对低焦油卷烟危害的认知”(OR=0.448,OR95%CI:0.327~0.612)4个变量与现在是否吸烟显著关联。结论湖南省疾控机构在职工作人员吸烟水平仍处于高水平,对烟草危害的知晓水平较低,男性、高年龄者、低学历者、对低焦油卷烟危害的认知错误者为吸烟行为高危人群。控烟工作应进一步加强。

Design of machine protection system for CAFe facility

Background In an accelerator-driven sub-critical system(ADS),a strong proton beam from a high-power proton accelerator irradiates a spallation target to produce very intense neutrons for driving a reactor.Currently,the China ADS front-end demo linac(CAFe)facility is being built to provide strong beam with the beam intensity of several mAs.Purpose This paper reports the machine protection system(MPS)of the CAFe facility,which prevents or minimizes the beam-induced damages or the damages from other sources to the machines in the accelerator.Method The MPS of CAFe facility works under the framework of experimental physics and industrial control system(EPICS).The developed machine protection system includes fast protection function and slow interlock function,where the fast function is performed by FPGA controller and the slow function is performed by PLC controller.Results Based on the reports on the status of all relevant subsystems,MPS will either perform an emergency shutdown of the beam or machine or will permit normal operation.When an emergency shutdown of the beam is needed,MPS switches off the ion source or switches on the chopper.In addition to its protective role,MPS will also trace the origin of an emergency shutdown.Conclusions The developed MPS has played an important role during the commissioning and successful operation of the CAFe facility at the end of 2019.

A new EPICS time-series data archiver using HBase

Background The control system of China ADS front-end demo linac(CAFe)is a distributed control system based on experimental physics and industrial control system(EPICS).The data generated by the control system are stored and retrieved by Channel Archiver.However,with the development of accelerator devices,its scale continues to expand.Channel Archiver has been unable to meet the increasing data volume in terms of availability,performance,and storage expansion.Purpose This paper presents a new data archiving system of EPICS control system.The archive engine is developed with microservice architecture,and the data storage layer uses the distributed database—HBase.Method The archive engine supports a variety of archive modes,and process variable(PV)archive requests and confguration changes can be realized without restarting.It can also realize data retrieval in a variety of ways.In addition,python scripts and Web UI can be used to implement the management functions of the system.Results Currently,the archiving system is under commissioning at CAFe facility.The results show that the new archiving system is convenient to operate and easy to expand and provides a research platform for future data analysis.