AIM:To study the influence of CXCR4/stromal cellderived factor-1(SDF-1) axis on E-cadherin/β-catenin complex expression in HT29 colon cancer cells and its underlying mechanisms.METHODS:Effect of SDF-1 on E-cadherin/...AIM:To study the influence of CXCR4/stromal cellderived factor-1(SDF-1) axis on E-cadherin/β-catenin complex expression in HT29 colon cancer cells and its underlying mechanisms.METHODS:Effect of SDF-1 on E-cadherin/β-catenin expression was detected by immunocytochemistry.E-cadherin and β-catenin mRNA expression levels were measured by reverse transcriptase-polymerase chain reaction.SDF-1-induced phosphorylation of phosphatidylinositol 3-kinase(PI3K)/AKT and β-catenin was detected by Western blotting.RESULTS:The E-cadherin and β-catenin mRNA expression levels in HT29 cells were lower 48 h after incubated with SDF-1 at the concentrations of 20 and 40 ng/mL(P < 0.05).SDF-1-induced significant phosphorylation of PI3K/AKT and β-catenin.AMD3100 and LY294002 inhibited the phosphorylation of PI3K/AKT and β-catenin.CONCLUSION:SDF-1 down-regulates the E-cadherin/β-catenin complex expression in HT29 cells by decreasing mRNA synthesis and increasing β-catenin phosphorylation.展开更多
基金Supported by National Natural Science Foundation of China,No. 30571712 and 30810403081
文摘AIM:To study the influence of CXCR4/stromal cellderived factor-1(SDF-1) axis on E-cadherin/β-catenin complex expression in HT29 colon cancer cells and its underlying mechanisms.METHODS:Effect of SDF-1 on E-cadherin/β-catenin expression was detected by immunocytochemistry.E-cadherin and β-catenin mRNA expression levels were measured by reverse transcriptase-polymerase chain reaction.SDF-1-induced phosphorylation of phosphatidylinositol 3-kinase(PI3K)/AKT and β-catenin was detected by Western blotting.RESULTS:The E-cadherin and β-catenin mRNA expression levels in HT29 cells were lower 48 h after incubated with SDF-1 at the concentrations of 20 and 40 ng/mL(P < 0.05).SDF-1-induced significant phosphorylation of PI3K/AKT and β-catenin.AMD3100 and LY294002 inhibited the phosphorylation of PI3K/AKT and β-catenin.CONCLUSION:SDF-1 down-regulates the E-cadherin/β-catenin complex expression in HT29 cells by decreasing mRNA synthesis and increasing β-catenin phosphorylation.
