Aim: To investigate the effect of formaldehyde (FA) on testes and the protective effect of vitamin E (VE) against oxidative damage by FA in the testes of adult rats. Methods: Thirty rats were randomly divided in...Aim: To investigate the effect of formaldehyde (FA) on testes and the protective effect of vitamin E (VE) against oxidative damage by FA in the testes of adult rats. Methods: Thirty rats were randomly divided into three groups: (1) control; (2) FA treatment group (FAt); and (3) FAt + VE group. FAt and FAt + VE groups were exposed to FA by inhalation at a concentration of 10 mg/m^3 for 2 weeks. In addition, FAt + VE group were orally administered VE during the 2-week FA treatment. After the treatment, the histopathological and biochemical changes in testes, as well as the quantity and quality of sperm, were observed. Results: The testicular weight, the quantity and quality of sperm, the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and glutathione (GSH) were significantly decreased whereas the level of malondialdehyde (MDA) was significantly increased in testes of rats in FAt group compared with those in the control group. VE treatment restored these parameters in FAt + VE group. In addition, microscopy with hematoxylin-eosin (HE) staining showed that seminiferous tubules atrophied, seminiferous epithelial cells disintegrated and shed in rats in FAt group and VE treatment significantly improved the testicular structure in FAt + VE group. Conclusion: FA destroys the testicular structure and function in adult rats by inducing oxidative stress, and this damage could be partially reversed by VE.展开更多
Most major oil zones in the Daqing Oilfield have reached a later,high water cut stage,but oil recovery is still only approximately 35%,and 50%of reserves remain to be recovered.The remaining oil is primarily distribut...Most major oil zones in the Daqing Oilfield have reached a later,high water cut stage,but oil recovery is still only approximately 35%,and 50%of reserves remain to be recovered.The remaining oil is primarily distributed at the edge of faults,in poor sand bodies,and in insufficiently injected and produced areas.Therefore,the edge of faults is a major target for remaining oil enrichment and potential tapping.Based on the dynamic change of production from development wells determined by the injection-recovery relationship at the edge of faults,we analyzed the control of structural features of faults on remaining oil enrichment at the edge.Our results show that the macroscopic structural features and their geometric relationship with sand bodies controlled remaining oil enrichment zones like the edges of NNE-striking faults,the footwalls of antithetic faults,the hard linkage segments(two faults had linked together with each other to form a bigger through-going fault),the tips of faults,and the oblique anticlines of soft linkages.Fault edges formed two types of forward microamplitude structures:(1) the tilted uplift of footwalls controlled by inverse fault sections and(2) the hanging-wall horizontal anticlines controlled by synthetic fault points.The remaining oil distribution was controlled by microamplitude structures.Consequently,such zones as the tilted uplift of the footwall of the NNW-striking antithetic faults with a fault throw larger than 40 m,the hard linkage segments,the tips of faults,and the oblique anticlines of soft linkage were favorable for tapping the remaining oil potential.Multi-target directional drilling was used for remaining oil development at fault edges.Reasonable fault spacing was determined on the basis of fault combinations and width of the shattered zone.Well core and log data revealed that the width of the shattered zone on the side of the fault core was less than 15 m in general;therefore,the distance from a fault to the development target should be larger than 15 m.Vertically segmented growth faults should take the separation of the lateral overlap of faults into account.Therefore,the safe distance of remaining oil well deployment at the fault edge should be larger than the sum of the width of shattered zone in faults and the separation of growth faults by vertical segmentation.展开更多
基金grants from the SciTechnical Development Project of Shaanxi Province, China (2005K15-G2) Traditional Chinese Project of Shaanxi Province, China (2005024) Natural Science Foundation of Xi' an Jiaotong University (573026).
文摘Aim: To investigate the effect of formaldehyde (FA) on testes and the protective effect of vitamin E (VE) against oxidative damage by FA in the testes of adult rats. Methods: Thirty rats were randomly divided into three groups: (1) control; (2) FA treatment group (FAt); and (3) FAt + VE group. FAt and FAt + VE groups were exposed to FA by inhalation at a concentration of 10 mg/m^3 for 2 weeks. In addition, FAt + VE group were orally administered VE during the 2-week FA treatment. After the treatment, the histopathological and biochemical changes in testes, as well as the quantity and quality of sperm, were observed. Results: The testicular weight, the quantity and quality of sperm, the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and glutathione (GSH) were significantly decreased whereas the level of malondialdehyde (MDA) was significantly increased in testes of rats in FAt group compared with those in the control group. VE treatment restored these parameters in FAt + VE group. In addition, microscopy with hematoxylin-eosin (HE) staining showed that seminiferous tubules atrophied, seminiferous epithelial cells disintegrated and shed in rats in FAt group and VE treatment significantly improved the testicular structure in FAt + VE group. Conclusion: FA destroys the testicular structure and function in adult rats by inducing oxidative stress, and this damage could be partially reversed by VE.
基金financial support from the Natural Science Foundation of China (Grant No. 41272151, 41472126)the Natural Science Foundation for Distinguished Young Scholars of Heilongjiang Province, China (Grant No. JC201304)+1 种基金the Joint Funds of the National Natural Science Foundation of China (Grant No. U1562214)the Program for Huabei Oilfield (Grant No. HBYT-CY5-2015-JS-127)
文摘Most major oil zones in the Daqing Oilfield have reached a later,high water cut stage,but oil recovery is still only approximately 35%,and 50%of reserves remain to be recovered.The remaining oil is primarily distributed at the edge of faults,in poor sand bodies,and in insufficiently injected and produced areas.Therefore,the edge of faults is a major target for remaining oil enrichment and potential tapping.Based on the dynamic change of production from development wells determined by the injection-recovery relationship at the edge of faults,we analyzed the control of structural features of faults on remaining oil enrichment at the edge.Our results show that the macroscopic structural features and their geometric relationship with sand bodies controlled remaining oil enrichment zones like the edges of NNE-striking faults,the footwalls of antithetic faults,the hard linkage segments(two faults had linked together with each other to form a bigger through-going fault),the tips of faults,and the oblique anticlines of soft linkages.Fault edges formed two types of forward microamplitude structures:(1) the tilted uplift of footwalls controlled by inverse fault sections and(2) the hanging-wall horizontal anticlines controlled by synthetic fault points.The remaining oil distribution was controlled by microamplitude structures.Consequently,such zones as the tilted uplift of the footwall of the NNW-striking antithetic faults with a fault throw larger than 40 m,the hard linkage segments,the tips of faults,and the oblique anticlines of soft linkage were favorable for tapping the remaining oil potential.Multi-target directional drilling was used for remaining oil development at fault edges.Reasonable fault spacing was determined on the basis of fault combinations and width of the shattered zone.Well core and log data revealed that the width of the shattered zone on the side of the fault core was less than 15 m in general;therefore,the distance from a fault to the development target should be larger than 15 m.Vertically segmented growth faults should take the separation of the lateral overlap of faults into account.Therefore,the safe distance of remaining oil well deployment at the fault edge should be larger than the sum of the width of shattered zone in faults and the separation of growth faults by vertical segmentation.