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乌参醒脑汤对帕金森病大鼠行为学及内质网应激PERK/ATF4通路的影响 被引量:1
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作者 于楠楠 毕海洋 +1 位作者 匡禹霏 孙殿甲 《中国现代医学杂志》 CAS 北大核心 2022年第14期35-41,共7页
目的探究乌参醒脑汤对帕金森病大鼠行为学及内质网应激PERK/ATF4通路的影响。方法将48只大鼠随机分为对照组、模型组、低剂量组、高剂量组,每组12只。除对照组外,另3组在大脑纹状体内注射0.2%6-羟基多巴胺(6-OHDA)复制帕金森病模型。模... 目的探究乌参醒脑汤对帕金森病大鼠行为学及内质网应激PERK/ATF4通路的影响。方法将48只大鼠随机分为对照组、模型组、低剂量组、高剂量组,每组12只。除对照组外,另3组在大脑纹状体内注射0.2%6-羟基多巴胺(6-OHDA)复制帕金森病模型。模型复制成功后,低剂量组和高剂量组(5.4 g/kg和10.8 g/kg)乌参醒脑汤连续灌胃给药21 d,给药结束后,通过旋转实验和旷场实验观察各组大鼠行为学表现;取大鼠纹状体,通过苏木精-伊红(HE)染色,观察其组织病理变化;采用实时荧光定量聚合酶链反应和Western blotting检测大鼠纹状体蛋白激酶R样内质网激酶(PERK)、转录活化因子4(ATF4)、C/EBP同源蛋白(CHOP)和B淋巴细胞瘤-2基因(Bax)mRNA和蛋白的表达。结果与对照组比较,模型组大鼠旋转频率、水平运动频率降低(P<0.05);与模型组比较,低剂量组和高剂量组大鼠旋转频率降低(P<0.05),水平运动频率升高(P<0.05);与低剂量组比较,高剂量组大鼠旋转频率降低(P<0.05),水平运动频率升高(P<0.05)。HE染色结果显示,对照组大鼠纹状体多巴胺能神经细胞元形态正常,结构和轮廓清晰,且数量多。模型组大鼠纹状体多巴胺能神经元细胞数量很少,形态破损严重,多成角状。低剂量组和高剂量组大鼠纹状体多巴胺能神经元细胞数量较多,结构比较完整,轮廓清晰。与对照组比较,模型组PERK、ATF4、CHOP、Bax mRNA和蛋白相对表达量升高(P<0.05);与模型组比较,低剂量组和高剂量组PERK、ATF4、CHOP、Bax mRNA和蛋白相对表达量降低(P<0.05);与低剂量组比较,高剂量组PERK、ATF4、CHOP、Bax mRNA和蛋白相对表达量降低(P<0.05)。结论乌参醒脑汤可以改善帕金森病大鼠的运动失调,修复损伤的多巴胺能神经元,其作用机制可能与抑制内质网应激PERK/ATF4通路有关。 展开更多
关键词 帕金森病 乌参醒脑汤 行为学 蛋白激酶R样内质网激酶 转录活化因子4 大鼠
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Clinical effect on knee osteoarthritis treated with Fu’s subcutaneous needling therapy 被引量:7
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作者 Zheng LIU Lin MA hai-yang bi 《World Journal of Acupuncture-Moxibustion》 CSCD 2020年第1期29-32,共4页
Objective:To observe and compare the clinical effect on knee osteoarthritis(KOA)between Fu’s subcutaneous needling therapy(FSN)and convention acupuncture.Methods:A total of 80 outpatients with KOA were divided into a... Objective:To observe and compare the clinical effect on knee osteoarthritis(KOA)between Fu’s subcutaneous needling therapy(FSN)and convention acupuncture.Methods:A total of 80 outpatients with KOA were divided into a FSN group(40 cases)and a conventional acupuncture group(40 cases)according to random number table.Within 1 week of treatment,FSN was used once every two days in the FSN group.The needle tip of Fu’s subcutaneous needle was inserted toward the affected muscle,about 5 mm in depth,at the angle of 15 to 25°.After the needle body lifted slightly,the needle went forward subcutaneously for 25 to 35 mm in depth and was swiped side to side horizontally with even exertion for about 1 min.According to the distribution of affected muscle,the reperfusion approach was repeated for 3 times consecutively.In the conventional acupuncture group conventional acupuncture was given,once a day,for 6 times totally.The scores of Western Ontario and McMaster universities osteoarthritis index(WOMAC)and Lysholm knee scoring scale(Lysholm)were compared between the two groups before and after 1-week treatment.The clinical effect of the two groups was analyzed statistically and evaluated.Results:After 1-week treatment with FSN,in the FSN group,the score of WOMACpain was reduced to be7.7±1.9 from 18.5±3.2,the score of WOMACstiffnessto be 1.5±0.7 from 4.5±1.8,WOMACfunction to be22.7±3.9 from 45.7±2.7 and the score of WOMACoverall to be 31.9±5.3 from 69.7±6.5.The differences were significant in comparison before and after treatment(all P<0.05).In the conventional acupuncture group,after 1-week treatment with conventional acupuncture,the score of WOMACpain was reduced to be11.3±2.8 from 18.2±3.0,the score of WOMACstiffness to be 3.0±1.6 from 5.6±1.7,WOMACfunction to be29.8±5.1 from 44.3±2.9 and the score of WOMACoverall to be 44.1±7.8 from 69.1±7.3.The differences were significant in comparison before and after treatment(all P<0.05).WOMAC score of every item in the FSN group was lower obviously than that in the conventional acupuncture group(P<0.01).After 1-week treatment,Lysholm score was increased to be 78.52±18.4 from 59.64±18.3 in the FSN group and it was to be 69.27±11.9 from 58.17±12.5 in the conventional acupuncture group.The differences were significant in comparison before and after treatment in either group(both P<0.05).Lysholm score in the FSN group was higher than that in the conventional acupuncture group(P<0.05).The total effective rate was 92.5%in the FSN group,higher than 77.5%in the conventional acupuncture group(P<0.05).Conclusion:Fu’s subcutaneous needling therapy effectively relieves the clinical symptoms of KOA and improves knee joint function.The therapeutic effect is better than that of the regular acupuncture. 展开更多
关键词 Fu’s SUBCUTANEOUS NEEDLING LOOSE CONNECTIVE tissue REPERFUSION approach Knee osteoarthritis
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