In order to distinguish eight carnation cultivars, 40 samples were analyzed by Fourier transform infrared (FI'IR) spectroscopy combined with principal component analysis (PCA) and hierarchical cluster analysis (...In order to distinguish eight carnation cultivars, 40 samples were analyzed by Fourier transform infrared (FI'IR) spectroscopy combined with principal component analysis (PCA) and hierarchical cluster analysis (HCA). According to the results, infrared spectra of eight carnation cuhivars were similar, but signifi- cant differences were observed in wave numbers and absorption peak intensities in the range of 1 800 -700cm-1. The second order derivative spectra in the range of 1 800 -700 cm -l were selected to perform principal component analysis (PCA) and hierarchical cluster analysis (HCA). The cumulative contribution rate of the first three principal components reached 96.2%. The classification accuracy rate of PCA and HCA was 95% and 100%, respectively. The results demonstrated that Fourier transform infrared (FTIR) spectroscopy combined with principal component analysis (PCA) and hierarchical cluster analysis (HCA) could be used for identification of different carnation cultivars.展开更多
This corrects the article“Delivery of coenzyme Q10 loaded micelle targets mitochondrial ROS and enhances efficiency of mesenchymal stem cell therapy in intervertebral disc degeneration”in volume 23 on page 247.The a...This corrects the article“Delivery of coenzyme Q10 loaded micelle targets mitochondrial ROS and enhances efficiency of mesenchymal stem cell therapy in intervertebral disc degeneration”in volume 23 on page 247.The authors regret that the printed version of the above article contains some errors which are not identified during the proofing stage.展开更多
Stem cell transplantation has been proved a promising therapeutic instrument in intervertebral disc degeneration(IVDD).However,the elevation of oxidative stress in the degenerated region impairs the efficiency of mese...Stem cell transplantation has been proved a promising therapeutic instrument in intervertebral disc degeneration(IVDD).However,the elevation of oxidative stress in the degenerated region impairs the efficiency of mesenchymal stem cells(BMSCs)transplantation treatment via exaggeration of mitochondrial ROS and promotion of BMSCs apoptosis.Herein,we applied an emulsion-confined assembly method to encapsulate Coenzyme Q10(Co-Q10),a promising hydrophobic antioxidant which targets mitochondria ROS,into the lecithin micelles,which renders the insoluble Co-Q10 dispersible in water as stable colloids.These micelles are injectable,which displayed efficient ability to facilitate Co-Q10 to get into BMSCs in vitro,and exhibited prolonged release of Co-Q10 in intervertebral disc tissue of animal models.Compared to mere use of Co-Q10,the Co-Q10 loaded micelle possessed better bioactivities,which elevated the viability,restored mitochondrial structure as well as function,and enhanced production of ECM components in rat BMSCs.Moreover,it is demonstrated that the injection of this micelle with BMSCs retained disc height and alleviated IVDD in a rat needle puncture model.Therefore,these Co-Q10 loaded micelles play a protective role in cell survival and differentiation through antagonizing mitochondrial ROS,and might be a potential therapeutic agent for IVDD.展开更多
基金Supported by National Natural Science Foundation of China"Vibration Spectrum-based Diagnosis of Biological Diseases in Broad Bean"(30960179)Science and Technology Innovation Program for Universities in Yunnan Province
文摘In order to distinguish eight carnation cultivars, 40 samples were analyzed by Fourier transform infrared (FI'IR) spectroscopy combined with principal component analysis (PCA) and hierarchical cluster analysis (HCA). According to the results, infrared spectra of eight carnation cuhivars were similar, but signifi- cant differences were observed in wave numbers and absorption peak intensities in the range of 1 800 -700cm-1. The second order derivative spectra in the range of 1 800 -700 cm -l were selected to perform principal component analysis (PCA) and hierarchical cluster analysis (HCA). The cumulative contribution rate of the first three principal components reached 96.2%. The classification accuracy rate of PCA and HCA was 95% and 100%, respectively. The results demonstrated that Fourier transform infrared (FTIR) spectroscopy combined with principal component analysis (PCA) and hierarchical cluster analysis (HCA) could be used for identification of different carnation cultivars.
文摘This corrects the article“Delivery of coenzyme Q10 loaded micelle targets mitochondrial ROS and enhances efficiency of mesenchymal stem cell therapy in intervertebral disc degeneration”in volume 23 on page 247.The authors regret that the printed version of the above article contains some errors which are not identified during the proofing stage.
基金This work was supported by National Natural Science Foundation of China(Grant No.82072478 to Yunpeng Zhao)Shandong Provincial Natural Science Foundation(Grant No.ZR2020YQ54,ZR019MH05 to Yunpeng Zhao)+4 种基金National Natural Science Foundation of China(Grant No.81874022,82172483 to Xinyu Liu)National Natural Science Foundation of China(Grant No.81972128to Xuesong Zhang)Application of Clinical Features of Capital City of Science and Technology Commission China BEIJING Special subject(Z181100001718180 to Xuesong Zhang)National Nature Science Foundation(82102522 to Lianlei Wang)Shandong Natural Science Foundation(ZR202102210113 to Lianlei Wang)and Shandong Province Taishan Scholar Project.
文摘Stem cell transplantation has been proved a promising therapeutic instrument in intervertebral disc degeneration(IVDD).However,the elevation of oxidative stress in the degenerated region impairs the efficiency of mesenchymal stem cells(BMSCs)transplantation treatment via exaggeration of mitochondrial ROS and promotion of BMSCs apoptosis.Herein,we applied an emulsion-confined assembly method to encapsulate Coenzyme Q10(Co-Q10),a promising hydrophobic antioxidant which targets mitochondria ROS,into the lecithin micelles,which renders the insoluble Co-Q10 dispersible in water as stable colloids.These micelles are injectable,which displayed efficient ability to facilitate Co-Q10 to get into BMSCs in vitro,and exhibited prolonged release of Co-Q10 in intervertebral disc tissue of animal models.Compared to mere use of Co-Q10,the Co-Q10 loaded micelle possessed better bioactivities,which elevated the viability,restored mitochondrial structure as well as function,and enhanced production of ECM components in rat BMSCs.Moreover,it is demonstrated that the injection of this micelle with BMSCs retained disc height and alleviated IVDD in a rat needle puncture model.Therefore,these Co-Q10 loaded micelles play a protective role in cell survival and differentiation through antagonizing mitochondrial ROS,and might be a potential therapeutic agent for IVDD.