Statistical analysis and interpretation on lightning impulse breakdown voltages of ester liquids under varying field uniformity

Ester liquids(ELs),as the alternative transformer liquids to the conventional mineral oils(MOs),have been increasingly used in power transformers with high voltage ratings.Among various properties,the lightning impulse breakdown voltages(LIBDVs)have been studied elaborately in the past two decades under different electric field uniformities.The electric field uniformities do have an effect on the breakdown characteristics,for example,breakdown mechanisms,polarity effects,and differences of LIBDV between ELs and MOs,which is quantitatively investigated based on the LIBDVs collected from the literatures.The breakdown mechanism transition phenomenon between streamer initiation dominated breakdown and streamer propagation dominated breakdown has been reviewed firstly.Then,the transition field factor boundaries to observe the polarity effect on LIBDVs are taken at around 20 for ELs and 10 for MOs.Besides,the transition field factor boundaries to observe the LIBDV distinctions between ELs and MOs are about 109 and 14 under positive and negative lightning impulse,respectively.The systematic analysis and interpretation on the transition field factor boundaries among different phenomena can provide insights on insulation design of ELs filled power transformers.

Derivation and validation of a similarity law for free-flight wind tunnel tests of parallel stage separation

Aiming at the safety problem of the stage separation of parallel reusable high-speed air vehicles,this paper studies the unsteady test method and focuses on deriving a similarity law of parallel stage separation free-flight wind tunnel tests.The new similarity law considers the influences of aerodynamic force and gravity on the motions of the two stages,as well as the influence of aerodynamic interference between the two stages on each other’s motion.From the perspective of multiangle physical equations,the conditions to ensure that the two-stage separation trajectory of a wind tunnel test is similar to that of a real air vehicle are derived innovatively,so as to ensure the authenticity and credibility of wind tunnel test results.The similarity law is verified by an HIFiRE-5 air vehicle,and the separation trajectories of wind tunnel tests and the real air vehicle are obtained by numerical simulation.The research shows that the similarity law derived in this paper can ensure that wind tunnel free-flight tests have the ability to predict the two-stage separation characteristics of real parallel vehicles.By analyzing the separation trajectory curve of the typical state,it is found that the new method can ensure that the trajectory error of a wind tunnel test does not exceed 1%,which indicates that this method is credible.The establishment of the new method lays the foundation for subsequent wind tunnel tests and provides support for research on the safety of the stage separation of parallel reusable air vehicles.

糜蛋白酶提高膜蛋白质谱分析序列覆盖率的胶内消化方法

近年来,质谱技术在膜蛋白结构与功能研究中被广泛应用。由于膜蛋白的跨膜结构域含有大量疏水性氨基酸,常常导致液质串联质谱检测的序列覆盖率较低,从而限制了质谱技术在膜蛋白结构与功能研究中的应用。文中利用人的整合膜蛋白维生素K环氧化物还原酶为模型,优化胶内消化条件,建立了一种稳定提高膜蛋白质谱序列覆盖率的糜蛋白酶胶内消化方法。通过探索钙离子浓度、pH值和缓冲体系对序列覆盖率、检测特异肽段的总数和类型以及特异肽段大小的影响,发现在5–10 mmol/L钙离子浓度、pH 8.0–8.5的Tris-HCl缓冲液中,可以兼顾序列覆盖率和肽段的多样性。该方法可以使膜蛋白的质谱覆盖率达到80%以上,将在膜蛋白结构与功能、膜蛋白相互作用位点的鉴定以及膜蛋白与小分子药物结合位点的鉴定等研究中具有广泛的应用价值。