Chemical constituents from leaves of Jatropha curcas

Objective:To investigate the chemical constituents from the leaves of Jatropha curcas and evaluate their inhibition on lipopolysaccharide(LPS)-activated BV-2 microglia cells.Methods:The n-BuOH extract of the leaves of J.curcas was isolated by macroporous adsorption resin,silica gel,ODS,column chromatography and semi-preparative HPLC.The structures of the compounds were identified by MS,NMR,ECD,and other spectroscopic methods.In addition,anti-neuroinflammatory effects of isolated compounds were evaluated by measuring the production of nitric oxide(NO)in overactivated BV-2 cells.Results:Seventeen compounds,including(7R,8S)-crataegifin A-4-O-β-D-glucopyranoside(1),(8R,8’R)-arctigenin(2),arctigenin-4’-O-β-D-glucopyranoside(3),(-)-syringaresinol(4),syringaresinol-4’-O-β-Dglucopyranoside(5),(-)-pinoresinol(6),pinoresinol-4’-O-β-D-glucopyranoside(7),buddlenol D(8),(2R,3R)-dihydroquercetin(9),(2S,3S)-epicatechin(10),(2R,3S)-catechin(11),isovitexin(12),naringenin-7-O-β-D-glucopyranoside(13),chamaejasmin(14),neochamaejasmin B(15),isoneochamaejasmin A(16),and tomentin-5-O-β-D-glucopyranoside(17)were isolated and identified.Compounds 2,4and 8 significantly inhibited the release of NO in BV-2 microglia activated by LPS,with IC50values of18.34,29.33 and 26.30μmol/L,respectively.Conclusion:Compound 1 is a novel compound,and compounds 2,3,8,14–17 are isolated from Jatropha genus for the first time.In addition,the lignans significantly inhibited NO release and the inhibitory activity was decreased after glycosylation.