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The M Protein of SARS-CoV: Basic Structural and Immunological Properties 被引量:1
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作者 JunWang SiqiLiu +15 位作者 ChangqingZeng JianWang HuanmingYang YongwuHu JieWen LinTang haijunzhang XiaoweiZhang YahLi JingWang YujunHan GuoqingLi JianpingShi XiangjunTian FengJiang XiaoqianZhao 《Genomics, Proteomics & Bioinformatics》 SCIE CAS CSCD 2003年第2期118-130,共13页
We studied structural and immunological properties of the SARS-CoV M (membrane) protein, based on comparative analyses of sequence features, phylogenetic investigation, and experimental results. The M protein is predi... We studied structural and immunological properties of the SARS-CoV M (membrane) protein, based on comparative analyses of sequence features, phylogenetic investigation, and experimental results. The M protein is predicted to contain a triple-spanning transmembrane (TM) region, a single N-glycosylation site near its N-terminus that is in the exterior of the virion, and a long C-terminal region in the interior. The M protein harbors a higher substitution rate (0.6% correlated to its size) among viral open reading frames (ORFs) from published data. The four substitutions detected in the M protein, which cause non-synonymous changes, can be classified into three types. One of them results in changes of pI (isoelectric point) and charge, affecting antigenicity. The second changes hydrophobicity of the TM region, and the third one relates to hydrophilicity of the interior structure. Phylogenetic tree building based on the variations of the M protein appears to support the non-human origin of SARS-CoV. To investigate its immunogenicity, we synthesized eight oligopeptides covering 69.2% of the entire ORF and screened them by using ELISA (enzyme-linked immunosorbent assay) with sera from SARS patients. The results confirmed our predictions on antigenic sites. 展开更多
关键词 SARS-COV the M protein enzyme immunoassay ANTIGENICITY
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