The emergence of single-cell genomic and transcriptomic sequencing accelerates the development of single-cell epigenomic technologies,providing an unprecedented opportunity for decoding cell fate decisions largely enc...The emergence of single-cell genomic and transcriptomic sequencing accelerates the development of single-cell epigenomic technologies,providing an unprecedented opportunity for decoding cell fate decisions largely encoded in the epigenome.Recent advances in single-cell multimodality epigenomic technologies facilitate directly interrogating the reg-ulatory relationship between multi-layer molecular information in the same cell.In this review,we discuss recent progress in development of single-cell multimodality epigenomic technologies and applications in elucidating cellular diversifications in development and diseases,with a focus on protein-DNA interactomics and regulatory links between epigenome and tran-scriptome.Further,we provide perspective on the future direction of single-cell multiomics tool development as well as challenges facing ahead.展开更多
A ratiometric fluorescence probe,NClO,for the rapid and selective detection of HClO had been designed and synthesized based on a 1,8-naphthalimide derivative.Probe NClO displayed a red emission(λmax=615 nm).In the pr...A ratiometric fluorescence probe,NClO,for the rapid and selective detection of HClO had been designed and synthesized based on a 1,8-naphthalimide derivative.Probe NClO displayed a red emission(λmax=615 nm).In the presence of HClO,the solution of probe NClO gave off a strong green fluorescence(λem,max=520 nm)with a rapid response(within seconds).This probe had been applied to image HClO in living cells and zebra fish.展开更多
In this work, we presented a fluorescent probe(MCQ-DNBS) for selective and sensitive detection of biothiols based on a methylated chromenoquinoline(MCQ) derivative. Probe MCQ-DNBS was constructed by masking the OH gro...In this work, we presented a fluorescent probe(MCQ-DNBS) for selective and sensitive detection of biothiols based on a methylated chromenoquinoline(MCQ) derivative. Probe MCQ-DNBS was constructed by masking the OH group in MCQ with a common sensing unit, 2,4-dinitrobezensulfonate group(DNBS) for biothiols. Due to the photo-induced electron transfer(PET) process between MCQ and DNBS, this probe was weekly fluorescent. Upon the addition of biothiols(Cys, Hcy and GSH), this probe emitted a strong red fluorescence(λ_(em max)=613 nm) with a large Stokes shift(115 nm). In addition,fluorescence imaging of biothiols in living cells was successfully realized using MCQ-DNBS as a detector.展开更多
A coumarinocoumarin-based fluorescent probe,JCCA,was developed for the detection of N2 H4.JCCA exhibited a fast turn-on fluorescence enhancement in response to N2 H4 with good selectivity,sensitivity and a detection l...A coumarinocoumarin-based fluorescent probe,JCCA,was developed for the detection of N2 H4.JCCA exhibited a fast turn-on fluorescence enhancement in response to N2 H4 with good selectivity,sensitivity and a detection limit of 7.4 nmol/L.Significantly,JCCA displayed a good capability for visualizing N2 H4 in living cells and zebra fish.展开更多
基金supported by the National Key Research and Development Program of China(2021YFA1100101 and 2017YFA0103402)the National Natural Science Foundation of China(32025015 and 31771607)the Peking-Tsinghua Center for Life Sciences.
文摘The emergence of single-cell genomic and transcriptomic sequencing accelerates the development of single-cell epigenomic technologies,providing an unprecedented opportunity for decoding cell fate decisions largely encoded in the epigenome.Recent advances in single-cell multimodality epigenomic technologies facilitate directly interrogating the reg-ulatory relationship between multi-layer molecular information in the same cell.In this review,we discuss recent progress in development of single-cell multimodality epigenomic technologies and applications in elucidating cellular diversifications in development and diseases,with a focus on protein-DNA interactomics and regulatory links between epigenome and tran-scriptome.Further,we provide perspective on the future direction of single-cell multiomics tool development as well as challenges facing ahead.
基金supported by the National Natural Science Foundation of China(No.U1608222)the State Key Laboratory of Fine Chemicals(No.KF1606)the financial support from Fundamental Research Funds for the Central Universities of Central South University(No.2018zzts109)
文摘A ratiometric fluorescence probe,NClO,for the rapid and selective detection of HClO had been designed and synthesized based on a 1,8-naphthalimide derivative.Probe NClO displayed a red emission(λmax=615 nm).In the presence of HClO,the solution of probe NClO gave off a strong green fluorescence(λem,max=520 nm)with a rapid response(within seconds).This probe had been applied to image HClO in living cells and zebra fish.
基金supported by the National Natural Science Foundation of China (No.U1608222)Special Fund for Agroscientific Research in the Public Interest of China(No.201503108)+1 种基金Fundamental Research Funds for the Central Universities of Central South University (No. 2018zzts109)the State Key Laboratory of Chemo/Biosensing and Chemometrics (No. 2016005)
文摘In this work, we presented a fluorescent probe(MCQ-DNBS) for selective and sensitive detection of biothiols based on a methylated chromenoquinoline(MCQ) derivative. Probe MCQ-DNBS was constructed by masking the OH group in MCQ with a common sensing unit, 2,4-dinitrobezensulfonate group(DNBS) for biothiols. Due to the photo-induced electron transfer(PET) process between MCQ and DNBS, this probe was weekly fluorescent. Upon the addition of biothiols(Cys, Hcy and GSH), this probe emitted a strong red fluorescence(λ_(em max)=613 nm) with a large Stokes shift(115 nm). In addition,fluorescence imaging of biothiols in living cells was successfully realized using MCQ-DNBS as a detector.
基金supported by the National Natural Science Foundation of China (No.U1608222)Special Fund for Agroscientific Research in the Public Interest of China (No.201503108)+1 种基金the State Key Laboratory of Fine Chemicals (No.KF1606)the State Key Laboratory of Chemo/Biosensing and Chemometrics (No. 2016005)
文摘A coumarinocoumarin-based fluorescent probe,JCCA,was developed for the detection of N2 H4.JCCA exhibited a fast turn-on fluorescence enhancement in response to N2 H4 with good selectivity,sensitivity and a detection limit of 7.4 nmol/L.Significantly,JCCA displayed a good capability for visualizing N2 H4 in living cells and zebra fish.
