Objective: To investigate the effect of silencing CD105 and Ki67 gene expression on OVCAR3 cell line in ovarian cancer. Methods: According to cell transfection results, OVCAR3 cells were divided into CD105-siRNA, Ki67...Objective: To investigate the effect of silencing CD105 and Ki67 gene expression on OVCAR3 cell line in ovarian cancer. Methods: According to cell transfection results, OVCAR3 cells were divided into CD105-siRNA, Ki67-siRNA, CD105-siRNA+ Ki67-siRNA, negative control (NC1 and NC2) and control (non-transfection) groups. The effect of silencing CD105, Ki67 and their combined gene on proliferation, migration, invasion and apoptosis of human ovarian cancer cells can be studied and analyzed with MTT assay, wound-healing assay, Transwell chamber and flow cytometry. Results: Compared with their respective negative control and liposome control groups, proliferation, migration and invasion abilities of OVCAR3 cells were decreased obviously in CD105-siRNA, Ki67-siRNA and CD105-siRNA + Ki67-siRNA groups after gene silencing. Whereas, the most significant change was detected in CD105-siRNA + Ki67-siRNA group along with obviously increased apoptosis rate of cancer cells, and the differences were of statistical significance (p < .01, p < .05). Conclusions: Expression vectors of CD105-siRNA and Ki67-siRNA can inhibit the proliferation of OVCAR3 cells, reduce their abilities of migration and invasion, and induce apoptosis of tumor cells. The effect of combined gene silence will be more significant.展开更多
The prevalence of gestational subclinical hypothyroidism has been increasing with years, and it has become one of the common diseases happened to women during pregnancy in China. Gestational subclinical hypothyroidism...The prevalence of gestational subclinical hypothyroidism has been increasing with years, and it has become one of the common diseases happened to women during pregnancy in China. Gestational subclinical hypothyroidism can not only increase the incidence of adverse pregnancy outcomes, but also have a negative impact on the development of the offspring. Therefore, it is necessary to make an early detection, diagnosis and treatment.展开更多
A case of endometriosis of ureter in the Department of Gynaecology and Obstetrics of the Third Affiliated Hospital of Inner Mongolia Medical School was collected and analyzed on the basis of diagnosis, physical examin...A case of endometriosis of ureter in the Department of Gynaecology and Obstetrics of the Third Affiliated Hospital of Inner Mongolia Medical School was collected and analyzed on the basis of diagnosis, physical examination and treatment. The disease was rare due to its atypical clinical symptoms so that it was difficult to diagnose. The paper aims to expand our thinking and improve the gynecologists awareness and the level of diagnosis and treatment of rare diseases through the clinical analysis of the case.展开更多
文摘Objective: To investigate the effect of silencing CD105 and Ki67 gene expression on OVCAR3 cell line in ovarian cancer. Methods: According to cell transfection results, OVCAR3 cells were divided into CD105-siRNA, Ki67-siRNA, CD105-siRNA+ Ki67-siRNA, negative control (NC1 and NC2) and control (non-transfection) groups. The effect of silencing CD105, Ki67 and their combined gene on proliferation, migration, invasion and apoptosis of human ovarian cancer cells can be studied and analyzed with MTT assay, wound-healing assay, Transwell chamber and flow cytometry. Results: Compared with their respective negative control and liposome control groups, proliferation, migration and invasion abilities of OVCAR3 cells were decreased obviously in CD105-siRNA, Ki67-siRNA and CD105-siRNA + Ki67-siRNA groups after gene silencing. Whereas, the most significant change was detected in CD105-siRNA + Ki67-siRNA group along with obviously increased apoptosis rate of cancer cells, and the differences were of statistical significance (p < .01, p < .05). Conclusions: Expression vectors of CD105-siRNA and Ki67-siRNA can inhibit the proliferation of OVCAR3 cells, reduce their abilities of migration and invasion, and induce apoptosis of tumor cells. The effect of combined gene silence will be more significant.
文摘The prevalence of gestational subclinical hypothyroidism has been increasing with years, and it has become one of the common diseases happened to women during pregnancy in China. Gestational subclinical hypothyroidism can not only increase the incidence of adverse pregnancy outcomes, but also have a negative impact on the development of the offspring. Therefore, it is necessary to make an early detection, diagnosis and treatment.
文摘A case of endometriosis of ureter in the Department of Gynaecology and Obstetrics of the Third Affiliated Hospital of Inner Mongolia Medical School was collected and analyzed on the basis of diagnosis, physical examination and treatment. The disease was rare due to its atypical clinical symptoms so that it was difficult to diagnose. The paper aims to expand our thinking and improve the gynecologists awareness and the level of diagnosis and treatment of rare diseases through the clinical analysis of the case.
