Integrative analysis of the transcriptome and metabolome reveals the response mechanism to tomato spotted wilt virus

Tomato spotted wilt virus(TSWV)is an important virus that has rapidly spread throughout the world.TSWV seriously hinders the production of tomato(Solanum lycopersicum)and other plants.In order to discover more new genes and metabolites related to TSWV resistance in tomato plants,the genes and metabolites related to the resistance of tomato plants inoculated with TSWV were identified and studied herein.The tomato TSWV-resistance line YNAU335(335)and TSWV-susceptible lines NO5 and 96172I(961)were used as the transcriptome and metabolome research materials.Transcriptomic and metabolomic techniques were used to analyze the gene and metabolite response mechanisms to TSWV inoculation.A total of 3566,2951,and 2674 differentially expressed genes(DEGs)were identified in lines 335,NO5,and961,respectively.Meanwhile,208,228,and 273 differentially accumulated metabolites(DAMs)were identified in lines 335,NO5,and 961,respectively.In line 335,the number of DEGs was the highest,but the number of DAMs was lowest.Furthermore,903 DEGs and 94 DAMs were common to the response to TSWV in the three inbred lines.The 903 DEGs and 94 DAMs were mainly enriched in the plant hormone signal transduction and flavonoid synthesis pathways.In addition,many nucleotide-binding site-leucine-rich repeat genes and transcription factors were found that might be involved in the TSWV response.These results provide new insights into TSWV resistance mechanisms.

Classroom Teaching Evaluation of Chinese Painting as a Form of Fine Arts in Rural Junior High Schools

Teaching evaluation is an indispensable part of the teaching system of junior high school.In order to inherit the excellent traditional Chinese culture and arts,traditional Chinese painting has been paid more and more attention to in the fine arts curriculum of rural junior high school.This paper takes the classroom teaching evaluation of traditional Chinese painting as a form of fine arts in rural junior high school as the research object,and analyzes the problems existing in the classroom teaching evaluation of traditional Chinese painting in rural junior high school.This paper puts forward the corresponding improvement measures from the aspects of comprehensive evaluation object,concrete evaluation content,flexible evaluation language and diversified evaluation subjects,in order to improve the evaluation level of fine arts teachers'classroom teaching,improve students'aesthetic cognition and learning interest in traditional Chinese painting,and cultivate students'innovative thinking and spirit.

A Preliminary Study on the Teaching Evaluation Language of Art Teachers in Rural Middle Schools

Teachers'teaching evaluation language is an important part of art class teaching,which can directly affect students'interest in art class.Taking the teaching evaluation language of rural art teachers as the research object,this paper analyzes the main problems existing in teachers'teaching evaluation language through questionnaire survey,case analysis and interview.This paper puts forward some improvement strategies for teachers'teaching evaluation language from the aspects of management,concept and use,so as to provide some reference for junior middle school art teachers'teaching evaluation language.

Construction and Research of Formative Evaluation System in Fine Arts Classroom Teaching in Rural Primary Schools

With the popularization of quality education,the teaching evaluation of fine arts class in rural primary schools has become an important part of the new curriculum reform,which tests whether the teaching goals are effectively achieved.This paper takes the formative evaluation system of fine arts classroom teaching in rural primary schools as the research object,analyzes the research status of the formative evaluation system in rural primary schools,and expounds the theoretical basis and construction principles of the formative evaluation system.This paper constructs a formative evaluation system of fine arts classroom teaching in rural primary schools from the aspects of teachers and students inside and outside the classroom,teaching process and teaching effect,in order to effectively stimulate students'interest in learning and improve their aesthetic literacy.

Application of Multiple Evaluation in Art Classroom Teaching in Rural Primary Schools

With the reform and development of China's primary school art education,the art teaching activities have obtained constant improvement,and the multiple evaluation of art teaching plays an important role in the process of art teaching.Based on the current situation of the evaluation of art classroom teaching in rural primary schools,this article uses diversified evaluation methods,multi-dimensional evaluation subjects and multiple evaluation contents to change the traditional and single primary art classroom evaluation method,to strive to improve the quality of art classroom teaching and stimulate students'learning interests and adapt to the development needs of students with different personalities.