The plasmodesmata-associated β-1,3-glucanase gene GhPdBG regulates fiber development in cotton

Trichomes are specialized structures that originate from epidermal cells of organs in higher plants.The cotton fiber is a unique single-celled trichome that elongates from the seed coat epidermis.Cotton(Gossypium hirsutum)fibers and trichomes are models for cell differentiation.In an attempt to elucidate the intercellular factors that regulate fiber and trichome cell development,we identified a plasmodesmal β-1,3-glucanase gene(designated GhPdBG)controlling the opening and closing of plasmodesmata in cotton fibers.Structural and evolutionary analysis showed haplotypic variation in the promoter region of the GhPdBG gene among 352 cotton accessions,but high conservation in the coding region.GhPdBG was expressed predominantly in cotton fibers and localized to plasmodesmata(PD).Expression patterns of PdBG that corresponded to PD permeability were apparent during fiber development in G.hirsutum and G.barbadense.The PdBG-mediated opening-closure of PD appears to be involved in fiber development and may account for the contrasting fiber traits of these two species.Ectopic expression of GhPdBG revealed that it functions in regulating fiber and trichome length and/or density by modulating plasmodesmatal permeability.This finding suggests that plasmodesmal targeting of GhPdBG,as a switch of intercellular channels,regulates single-celled fiber and trichome development in cotton.

Research and application of the virtual simulation system teaching method in NC machining course

Virtual simulation technology has become one of the most popular technologies in the field of engineering education after the multimedia information technology in recent years.This paper,based on the comprehensive integrated simulation and verification module of UG NX software,describes and discusses a novel virtual simulation system teaching(VSST)for numerically controlled machining to support the student engineering training to achieve the theoretical knowledge and practical techniques in numerically controlled machining.The findings of a study designed to evaluate the impact of VSST for the development of numerically controlled machining course are presented here.In addition,analysis of the follow-up surveys indicates that the VSST method enables to provide the concrete experience of interaction between the students and the simulation environment and to further stimulate students’interest in learning,so that the students who used VSST achieve significantly higher results than their co-workers.