In recent years,peanut yield and quality are more seriously affected by pod rot disease in China.However,managing this disease has proven challenging due to the wide host range of its pathogens.In this study,four soil...In recent years,peanut yield and quality are more seriously affected by pod rot disease in China.However,managing this disease has proven challenging due to the wide host range of its pathogens.In this study,four soil samples were collected from fields with pod rot disease in Hebei Province,and 454 pyrosequencing was used to analyze the fungal communities structure within them.All 38490 ITS high-quality sequences were grouped into 1203 operational taxonomic units,the fungal community diversity of four soil samples was evaluated and compared using Shannon index and Simpson index.The results showed that members of Ascomycota were dominant,followed by Basidiomycota.According to the BLAST results at the species level,Guehomyces had the highest abundance,accounting for about 7.27%,followed by Alternaria,Fusarium,and Davidiella.The relative abundance of Fusarium oxysporum isolated from rotting peanuts in soil with peanut rot was higher than that in the control,indicating that Fusarium oxysporum might be one of the main pathogenic fungus of peanut rot in this area.This study delved into the broader fungal community associated with peanut pod rot,providing a theoretical foundation for preventing and treating this disease in agriculture.展开更多
In order to evaluation the efficiency of SRAP markers on genetic diversity of Aspergillus flavus,we screened out 17sets of primer pairs which could produce clear and reproducible SRAP bands from 150 SRAP primer pairs....In order to evaluation the efficiency of SRAP markers on genetic diversity of Aspergillus flavus,we screened out 17sets of primer pairs which could produce clear and reproducible SRAP bands from 150 SRAP primer pairs.The size of SRAP fragments ranged from 120 to 2100 bp.Primer pair Me10/Em9 produced the maximum number of polymorphic bands(12 bands),while Me8/Em13 produced the fewest number of polymorphic bands(only 1).Through analysis genetic diversity ability of different sets of primer pairs,the set of 12 primer pairs was selected for SRAP genetic marker of A.flavus.Cluster analysis was performed based on the genetic similarity coefficients,which ranged from 0.53 to 0.89.A dendrogram assembled using the unweighted pair-group method with arithmetic averages grouped A.flavus samples into 5 main clusters.The results suggested that SRAP marker is a useful molecular technology for the diversity of A.flavus from peanut soils in China.展开更多
基金supported by General project of Shandong Provincial Natural Science Foundation(ZR2020MC103,ZR2021MC040)Agricultural Innovation Project of Shandong Academy of Agricultural Sciences(CXGC2022B06,CXGC2022F33).
文摘In recent years,peanut yield and quality are more seriously affected by pod rot disease in China.However,managing this disease has proven challenging due to the wide host range of its pathogens.In this study,four soil samples were collected from fields with pod rot disease in Hebei Province,and 454 pyrosequencing was used to analyze the fungal communities structure within them.All 38490 ITS high-quality sequences were grouped into 1203 operational taxonomic units,the fungal community diversity of four soil samples was evaluated and compared using Shannon index and Simpson index.The results showed that members of Ascomycota were dominant,followed by Basidiomycota.According to the BLAST results at the species level,Guehomyces had the highest abundance,accounting for about 7.27%,followed by Alternaria,Fusarium,and Davidiella.The relative abundance of Fusarium oxysporum isolated from rotting peanuts in soil with peanut rot was higher than that in the control,indicating that Fusarium oxysporum might be one of the main pathogenic fungus of peanut rot in this area.This study delved into the broader fungal community associated with peanut pod rot,providing a theoretical foundation for preventing and treating this disease in agriculture.
基金the grants from the Natural Science Foundation of Shandong Province(ZR2020MC103,ZR2021MC040)Innovation Project of Agricultural Science and Technology of Shandong Academy of Agricultural Sciences(CXGC2022B06,CXGC2022F33)。
文摘In order to evaluation the efficiency of SRAP markers on genetic diversity of Aspergillus flavus,we screened out 17sets of primer pairs which could produce clear and reproducible SRAP bands from 150 SRAP primer pairs.The size of SRAP fragments ranged from 120 to 2100 bp.Primer pair Me10/Em9 produced the maximum number of polymorphic bands(12 bands),while Me8/Em13 produced the fewest number of polymorphic bands(only 1).Through analysis genetic diversity ability of different sets of primer pairs,the set of 12 primer pairs was selected for SRAP genetic marker of A.flavus.Cluster analysis was performed based on the genetic similarity coefficients,which ranged from 0.53 to 0.89.A dendrogram assembled using the unweighted pair-group method with arithmetic averages grouped A.flavus samples into 5 main clusters.The results suggested that SRAP marker is a useful molecular technology for the diversity of A.flavus from peanut soils in China.
