Recent studies have revealed that osthole,an active constituent isolated from the fruit of Cnidium monnieri（L.） Cusson,a traditional Chinese medicine,possesses anticancer activity.However,its effect on breast cancer...Recent studies have revealed that osthole,an active constituent isolated from the fruit of Cnidium monnieri（L.） Cusson,a traditional Chinese medicine,possesses anticancer activity.However,its effect on breast cancer cells so far has not been elucidated clearly.In the present study,we evaluated the effects of osthole on the proliferation,cell cycle and apoptosis of human breast cancer cells MDA-MB 435.We demonstrated that osthole is effective in inhibiting the proliferation of MDA-MB 435 cells,The mitochondrion-mediated apoptotic pathway was involved in apoptosis induced by osthole,as indicated by activation of caspase-9 and caspase-3 followed by PARP degradation.The mechanism underlying its effect on the induction of G1 phase arrest was due to the up-regulation of p53 and p21 and down-regulation of Cdk2 and cyclin D1 expression.Were observed taken together,these findings suggest that the anticancer efficacy of osthole is mediated via induction of cell cycle arrest and apoptosis in human breast cancer cells and osthole may be a potential chemotherapeutic agent against human breast cancer.展开更多
A new species of the genus Tipulodina Enderlein, 1912, Tipulodina simianshanensis Men sp. nov.(South China: Chongqing) is described and illustrated. A key to known species of the genus in China is provided. The new sp...A new species of the genus Tipulodina Enderlein, 1912, Tipulodina simianshanensis Men sp. nov.(South China: Chongqing) is described and illustrated. A key to known species of the genus in China is provided. The new species represents the first record of Tipulodina species from Chongqing, China.展开更多
Dear editor,Site specific recombinases have been widely used for genome engineering in cardiovascular research(Fu et al.,2024;Huang et al.,2023;Xue et al.,2022).In particular,Cre is the most broadly used recombinase f...Dear editor,Site specific recombinases have been widely used for genome engineering in cardiovascular research(Fu et al.,2024;Huang et al.,2023;Xue et al.,2022).In particular,Cre is the most broadly used recombinase for cell type-specific lineage tracing and gene manipulation in mammals.For smooth muscle cell(SMC)-specific targeting,several SMC promoters including Myh11(smooth muscle myosin heavy chain),Acta2(smooth muscle a-2 actin),and SM22a(Tagln)have been used to drive Cre or CreER.展开更多
基金supported by grant from the Natural Science Foundation of Jiangsu Province(No.BK2011140)
文摘Recent studies have revealed that osthole,an active constituent isolated from the fruit of Cnidium monnieri（L.） Cusson,a traditional Chinese medicine,possesses anticancer activity.However,its effect on breast cancer cells so far has not been elucidated clearly.In the present study,we evaluated the effects of osthole on the proliferation,cell cycle and apoptosis of human breast cancer cells MDA-MB 435.We demonstrated that osthole is effective in inhibiting the proliferation of MDA-MB 435 cells,The mitochondrion-mediated apoptotic pathway was involved in apoptosis induced by osthole,as indicated by activation of caspase-9 and caspase-3 followed by PARP degradation.The mechanism underlying its effect on the induction of G1 phase arrest was due to the up-regulation of p53 and p21 and down-regulation of Cdk2 and cyclin D1 expression.Were observed taken together,these findings suggest that the anticancer efficacy of osthole is mediated via induction of cell cycle arrest and apoptosis in human breast cancer cells and osthole may be a potential chemotherapeutic agent against human breast cancer.
基金supported by the National Natural Science Foundation of China(31300551)the Anhui Outstanding Young Talent Support Program(gxfx2017059)
文摘A new species of the genus Tipulodina Enderlein, 1912, Tipulodina simianshanensis Men sp. nov.(South China: Chongqing) is described and illustrated. A key to known species of the genus in China is provided. The new species represents the first record of Tipulodina species from Chongqing, China.
基金supported by the National Natural Science Foundation of China(81970412,82270415,82088101,32050087,82300552)Shanghai Municipal Science and Technology Commission Innovation Fund(22S31904800)+3 种基金Fujian Province Health Science and Technology Fund(2021GGB030)Fujian Natural Science Foundation Fund(2022J01410826)Shanghai Municipal Health Commission(20234Z0012)the New Cornerstone Science Foundation through the New Cornerstone Investigator Program and the XPLORER PRIZE。
文摘Dear editor,Site specific recombinases have been widely used for genome engineering in cardiovascular research(Fu et al.,2024;Huang et al.,2023;Xue et al.,2022).In particular,Cre is the most broadly used recombinase for cell type-specific lineage tracing and gene manipulation in mammals.For smooth muscle cell(SMC)-specific targeting,several SMC promoters including Myh11(smooth muscle myosin heavy chain),Acta2(smooth muscle a-2 actin),and SM22a(Tagln)have been used to drive Cre or CreER.
